pBA310
(Plasmid
#190002)
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Purpose(Empty Backbone) Inducible expression vector for Trypanosoma brucei
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190002 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBA310 (Derivative of pDEX777)
- Backbone size (bp) 6429
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Vector typeExpression vector for Trypanosoma brucei
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Selectable markersphleomycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This pBA310 is a modified version of the pDEX777 plasmid (Poon et al. 2012 Open Biology https://doi.org/10.1098/rsob.110037) and was originally published in Nerusheva and Akiyoshi 2016 Open Biology (https://doi.org/10.1098/rsob.150206)
NLS and several restriction enzyme sites were inserted between XbaI and BamHI sites of pDEX777 for overexpressing proteins of your interest with/without GFP and with/without NLS. GFP is in between HindIII and XbaI. NLS from Tbrucei La protein (RGHKRSRE) is located in between AgeI and MfeI.
With NLS:
You can clone your gene of interest using MfeI/EcoRV/NdeI/PacI/AscI/BclI/PmeI/BamHI/AflII
Without NLS:
You can clone your gene of interest using XbaI/ClaI/AgeI and PmlI/MfeI/EcoRV/NdeI/PacI/AscI/BclI/PmeI/BamHI/AflII
Make sure your gene of interest is inserted in frame with GFP and/or NLS. Include start or stop codon as appropriate.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBA310 was a gift from Bungo Akiyoshi (Addgene plasmid # 190002 ; http://n2t.net/addgene:190002 ; RRID:Addgene_190002) -
For your References section:
Targeted protein degradation using deGradFP in Trypanosoma brucei. Ishii M, Akiyoshi B. Wellcome Open Res. 2022 Jun 23;7:175. doi: 10.12688/wellcomeopenres.17964.1. eCollection 2022. 10.12688/wellcomeopenres.17964.1 PubMed 35865221