167,114 results

1. pTol2-UAS-zmiRFP

   Plasmid

   #108415

   Purpose

   Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   zmiRFP
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   UAS

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. MARS_pNIC-CTB10H

   Plasmid

   #153053

   Purpose

   Expression construct for producing Avi tagged Methionyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   MARS (MARS1 Human)
   

   Use

   Tags

   TEV-Avi-His10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV-hGFAP-SOX2-IRES-GFP

   Plasmid

   #183909

   Purpose

   Expression of human SOX2 and GFP under the human GFAP ABC1D promoter

   Depositor

   Chun-Li Zhang

   Insert

   SOX2 (GFAP, SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-SKII-3XHA-fCLIP-NAT

   Plasmid

   #188921

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-fCLIP-NAT
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. IDG_CACNG3_OE_1

   Plasmid

   #161622

   Purpose

   Overexpresses CACNG3

   Depositor

   Mike McManus

   Insert

   CACNG3 (CACNG3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLinker-BirA-3xHA-HXGPRT-loxP

   Plasmid

   #86668

   Purpose

   for generation of BirA tagged strains using CRISPR tagging technology

   Depositor

   David Sibley

   Insert

   Linker-BirA-3xHA-HXGPRT 3'UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330.puro

   Plasmid

   #110403

   Purpose

   CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

   Depositor

   Sandra Martha Gomes Dias

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p663-UBC-V5-BRSK1_IDG-K

   Plasmid

   #135270

   Purpose

   Gateway destination clone of BRSK1 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   V5-BRSK1 (BRSK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MEK1C222

   Plasmid

   #164638

   Purpose

   Bacterial expression plasmid for His6-MEK1C222

   Depositor

   Ben Davis

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
   

   Use

   Tags

   His6-tag

   Expression

   Bacterial

   Mutation

   G(-19)F/S222C/C277S/C376S

   Promoter

   T7

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-HisD-KRaION1

    Plasmid

    #177799

    Purpose

    Bacterial expression of green fluorescent potassium indicator KRaION1

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-SKII-3XHA-fSNAP-NAT

    Plasmid

    #188916

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-fSNAP-NAT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ATF4 2: Mouse ATF4 (CHOP11/cATF)-bZIP

    Plasmid

    #21849

    Depositor

    David Ron

    Insert

    ATF4 (Atf4 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    N-terminal transactivation domain deleted and rep…

    Promoter

    Available Since

    Sept. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-GFP

    Plasmid

    #160697

    Purpose

    Expresses GFP in mammalian cells

    Depositor

    Christine Mayr

    Article

    Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRG235

    Plasmid

    #64533

    Purpose

    Integrative E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28b-NmCas9-Flag

    Plasmid

    #109030

    Purpose

    Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL29

    Plasmid

    #15457

    Depositor

    Chia Lee

    Article

    Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

    Insert

    
    

    Use

    S. aureus-e. coli shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid

    #181843

    Purpose

    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of residues 12-112 in PKA RIα.

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-mCherry-Mango II x 24

    Plasmid

    #127587

    Purpose

    Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

    Depositor

    David Rueda

    Article

    Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

    Insert

    mCherry-Mango II x24
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE Tet ON

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MGP

    Plasmid

    #26526

    Depositor

    David Baltimore

    Article

    O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

    Plasmid

    #120564

    Purpose

    Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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