167,114 results

1. Str-KDEL_SBP-EGFP-ColX

   Plasmid

   #222305

   Purpose

   Synchronize the trafficking of CollagenX from the ER.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

   Insert

   Streptavidin-KDEL and CollagenX fused to SBP-EGFP (COL10A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   signal peptide of COL10A1 removed

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Str-KDEL_SBP-EGFP-gp135

   Plasmid

   #222306

   Purpose

   Synchronize the trafficking of gp135/podocalyxin from the ER.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

   Insert

   Streptavidin-KDEL and gp135 fused to SBP-EGFP (PODXL Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   signal peptide removed

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TOgw-N-mCherry

   Plasmid

   #223948

   Purpose

   Gateway cloning of genes of interest with an N-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

   Depositor

   Karla Feijs, Roko Žaja

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-shNC-mCherry

   Plasmid

   #222962

   Purpose

   Negative control for lentiviral shRNA

   Depositor

   Xinyu Zhao

   Article

   Guo et al Nat Commun. 2023 Jun 26;14(1):3801. doi:

   Insert

   Negative control
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-PunyEEER

   Plasmid

   #208322

   Purpose

   Bacterial expression of the His-tagged peptide PunyEEER.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   PunyERRE
   

   Use

   Tags

   DBCO-Tag, His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-TinyERRE

   Plasmid

   #208320

   Purpose

   Bacterial expression of the His-tagged peptide TinyERRE.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TinyERRE
   

   Use

   Tags

   DBCO-Tag, His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cav-B

   Plasmid

   #209879

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the caveolae with mouse CAVIN1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Use

   Tags

   CAVIN1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TREER-Actin

   Plasmid

   #208330

   Purpose

   TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TREER-Actin
   

   Use

   Tags

   TREER

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TOMM20-PuRRRE

   Plasmid

   #208329

   Purpose

   PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TOMM20-PuRRRE
   

   Use

   Tags

   PuRRRE

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBVboostFGII WPRE 2A-CVB3

    Plasmid

    #202076

    Purpose

    Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    CVB3 2A protease with HisTag
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    polh, CAG, T7

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJR017

    Plasmid

    #198835

    Purpose

    In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::TeTX-SL2-mKate::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-Spe4-Kozak-miRFP713-RfA

    Plasmid

    #186381

    Purpose

    Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDx_mCherry-SYFP2

    Plasmid

    #189759

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mCherry-SYFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SFRS10

    Plasmid

    #156007

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SFRS10 (TRA2B Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. F40F8.11 RNAi

    Plasmid

    #186731

    Purpose

    Knockdown of F40F8.11 gene

    Depositor

    Seung-Jae Lee

    Insert

    F40F8.11 targeting RNA
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-Spe4-RfA-miRFP713-stop

    Plasmid

    #186391

    Purpose

    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-SKII-3XHA-eDHFR-NAT

    Plasmid

    #188926

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-eDHFR-NAT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB-Halo,IRES-NGFR

    Plasmid

    #164520

    Purpose

    All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_2pabPAC(Y72L)

    Plasmid

    #158970

    Purpose

    A negative control for AAV-mediated 2P-activatable bPAC

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

    Insert

    2pabPACY72L
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

    Plasmid

    #120565

    Purpose

    Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K, T35A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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