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Plasmid#74282PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseTagsExpressionMutationPromoterAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_Giantin_C1
Plasmid#85048PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX304_CMV::mCherry-LgBiT-FLAG-NES
Plasmid#199715PurposeExpresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-LgBiT-FLAG-NES
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE 80L Kan T7WT
Plasmid#102790PurposeExpresses T7 RNA polymeraseDepositorInsertT7 RNA polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAVS1-MAFB-IRF8-CEBPA
Plasmid#190720PurposeFor microglia differentiation from iPSCDepositorInsertMAFB-IRF8-CEBPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN251
Plasmid#188471PurposeLentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)DepositorInsertenCas12a-HF (gift from Dr. Keith Joung)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-CaMKIIa-EGFP (AAV9)
Viral Prep#50469-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsEGFPAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML104-KanMx4
Plasmid#83476PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.DepositorInsertKanMX4
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
ER-B
Plasmid#209870PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddGFP B
UseTagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1
Plasmid#187579PurposeExpress FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cellsDepositorInsertPSPC1 (PSPC1 Human)
UseTagsAPEX2-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-RPL9
Plasmid#31334DepositorInsertribosomal protein L9 (RPL9 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP (AAV2)
Viral Prep#50465-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3
Plasmid#209727PurposeExpresses cytosolic medium affinity ratiometric ATP sensorDepositorInsert(cyto).iATPSnFR2.A95K.miRFP670nano3
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_221
Plasmid#169142PurposeConstitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.DepositorInsertEGFP , EGFP sgRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pKNOCK-Km
Plasmid#46262PurposeBacterial targetingDepositorTypeEmpty backboneUseBacterial targetingTagsExpressionMutationPromoterAvailable sinceAug. 14, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
FZ-NbPDS
Plasmid#184268PurposepAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.DepositorInsertCPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
UseTagsExpressionPlantMutationThe protease processing site between MP and L-CP …PromoterCaMV 35S with duplicated enhancerAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUIN
Plasmid#184267PurposeA binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.DepositorInsertCPSMV RNA1 cDNA with 4 introns
UseTagsExpressionPlantMutationS1098P, K1651RPromoterCaMV 35S with duplicated enhancerAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.chFP.W
Plasmid#51008PurposeLentivector that expresses chFP from internal human PGK promoterDepositorInsertmChFP
UseLentiviralTagsExpressionMammalianMutationPromoterhuman PGKAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only