LV-hGFAP-SOX2-IRES-GFP
(Plasmid
#183909)
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PurposeExpression of human SOX2 and GFP under the human GFAP ABC1D promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCS-CDF-CG-PRE
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Not published but this plasmid was based on the following publication:
In vivo reprogramming of astrocytes to neuroblasts in the adult brain. Niu W, Zang T, Zou Y, Fang S, Smith DK, Bachoo R, Zhang CL. Nat Cell Biol. 2013 Oct;15(10):1164-75. doi: 10.1038/ncb2843. Epub 2013 Sep 22. https://pubmed.ncbi.nlm.nih.gov/24056302/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LV-hGFAP-SOX2-IRES-GFP was a gift from Chun-Li Zhang (Addgene plasmid # 183909 ; http://n2t.net/addgene:183909 ; RRID:Addgene_183909)