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Addgene

LV-hGFAP-SOX2-IRES-GFP
(Plasmid #183909)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183909 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    CS-CDF-CG-PRE
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SOX2
  • Species
    H. sapiens (human)
  • Entrez Gene
    GFAP (a.k.a. ALXDRD)
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Not published but this plasmid was based on the following publication:

In vivo reprogramming of astrocytes to neuroblasts in the adult brain. Niu W, Zang T, Zou Y, Fang S, Smith DK, Bachoo R, Zhang CL. Nat Cell Biol. 2013 Oct;15(10):1164-75. doi: 10.1038/ncb2843. Epub 2013 Sep 22. https://pubmed.ncbi.nlm.nih.gov/24056302/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LV-hGFAP-SOX2-IRES-GFP was a gift from Chun-Li Zhang (Addgene plasmid # 183909 ; http://n2t.net/addgene:183909 ; RRID:Addgene_183909)