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Plasmid#195505PurposeT-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17DepositorInsertpT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
UseCRISPR; Donor vectorTagsmRuby-3xFLAG-NLS-3xSV40-poly(A)ExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-hRPH3A_c.1853Gmut
Plasmid#190479Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
UseTagsExpressionMammalianMutationc.1853A>GPromoterSV40Available sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA
Plasmid#108735PurposeExpresses N-terminus to aa256 of Cre recombinase fused to FRB CIDDepositorInsertCre recombinase fragment N-terminus to aa256
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-U2B''-fSNAPc
Plasmid#106988Purposeopen reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sitesDepositorInsertUB2" (SNRPB2 Human)
UseTagsfSNAPExpressionMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM5080
Plasmid#85106PurposePtrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expresDepositorInsertYFP-KaiC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
BASIC_11_RBS34-GFP-B0015
Plasmid#68145Purposestorage vector for BASIC part: RBS-GFP(ORF)DepositorInsertRBS-34-GFP-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-APEX2-C Cloning Vector
Plasmid#128146PurposeTo express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEMDepositorTypeEmpty backboneUseTagsFlag-APEX2ExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (AAV1)
Viral Prep#100834-AAV1PurposeReady-to-use AAV1 particles produced from pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 (#100834). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 plasmid DNA. CamKII-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsNoneAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK522-FAST-PETase
Plasmid#214754Purposeplasmid for expression FAST-PETase in Pseudomonas putidaDepositorInsertFAST-PETase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-PPARdelta
Plasmid#16529DepositorInsertPPAR delta (PPARD Human)
UseAdenoviralTags2x HA (YDVPDYAS)ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR
Plasmid#213481PurposeAAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsGFP-KASH
U6-gRNA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-hYAP1
Plasmid#17843DepositorInsertYAP (YAP1 Human)
UseTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-scrCdh20.1 GFP
Plasmid#209106PurposeGFP expressing scramble of shRNA targeting Cdh20DepositorInsertscrCdh20.1
UseTagsExpressionMutationPromoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt (AAV1)
Viral Prep#175181-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt (#175181). In addition to the viral particles, you will also receive purified pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt plasmid DNA. Synapsin-driven, Cre-dependent, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-15F11-HA-mCh
Plasmid#129591PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCELF4-4405
Plasmid#115466PurposeConstitutive lentiviral expressionDepositorInsertshCELF4-4405 (CELF4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cyto-roGFP
Plasmid#49435PurposeExpresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cellsDepositorInsertroGFP2
UseAdenoviralTagsExpressionMammalianMutationC48S, Q80R, S147C, and Q204C in Clontech's G…PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-GRAB_ACh3.0
Plasmid#121922Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0DepositorHas ServiceAAV9InsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV Retrograde)
Viral Prep#162378-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only