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pNTK human MBD3 PGK-Neo-pA flox

Plasmid

#52362

Purpose

Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

MBD3 (MBD3 Human)

Use

Targeting

Tags

Expression

Mutation

Promoter

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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PB-PGK-Cit-hcvs-DHFR

Plasmid

#116044

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-hcvs-DHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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p130Cas

Plasmid

#186131

Purpose

For expression of recombinant p130Cas in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Use

Tags

His6

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PGK-tvD-Cit

Plasmid

#116043

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

tvD-Cit

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-UHMK1

Plasmid

#23433

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

UHMK1 (UHMK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC26A8

Plasmid

#132277

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A8 (SLC26A8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-TurboCas-NLS-Blast

Plasmid

#232346

Purpose

Lentiviral expression vector for dCas9 fused to miniTurboNLS

Depositor

Ali Shilatifard

Article

Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA, miniTurbo, V5 and 3xSV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag ADCK4

Plasmid

#20544

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ADCK4 (ADCK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pAG223 PRKD1 3'UTR mut

Plasmid

#12042

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

PRKD1 3'UTR mut (PRKD1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pAG225 HIPK1 3'UTR mut

Plasmid

#12044

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

HIPK1 3'UTR mut (HIPK1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pAG201 HIPK1 3'UTR

Plasmid

#12012

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

HIPK1 3'UTR (HIPK1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG145 HIPK1 3'UTR

Plasmid

#12003

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N10 3'UTR (HIPK1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG177 HIPK1 3'UTR mut

Plasmid

#12035

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N10 3'UTR mut (HIPK1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-CR029

Plasmid

#234041

Purpose

expresses evoCDA1 BE4max base editor in a human T cell optimized lentiviral transfer plasmid

Depositor

Alexander Marson

Article

Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

Insert

evoCDA1-Cas9(D10A)-P2A-blast

Use

Lentiviral

Tags

blast

Expression

Mutation

Promoter

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CR033

Plasmid

#234042

Purpose

expresses ABE8e(V106W) base editor in a human T cell optimized lentiviral transfer plasmid

Depositor

Alexander Marson

Article

Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

Insert

TadA8e(V106W)-Cas9(D10A)-P2A-blast

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CR102

Plasmid

#234043

Purpose

expresses ABE8e(V106W) - SpG Cas9 base editor in a human T cell optimized lentiviral transfer plasmid

Depositor

Alexander Marson

Article

Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

Insert

TadA8e(V106W)-SpG-Cas9(D10A)-P2A-blast

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-LNI

Plasmid

#233205

Purpose

Lentiviral dox-inducible expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Isl1, Lhx3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:MYRFL_CE_pISceI

Plasmid

#223045

Purpose

Express MYRFL gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

MYRFL

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP Keima-Pex11B

Plasmid

#224558

Purpose

expresses Keima fused to PEX11B in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

PEX11B (PEX11B Human)

Use

Retroviral

Tags

Keima

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP APEX-PEX26

Plasmid

#224562

Purpose

expresses APEX fused to PEX26 peroxisomal localization sequence in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

PEX26 (PEX26 Human)

Use

Retroviral

Tags

APEX

Expression

Mammalian

Mutation

PEX26 peroxisomal targeting sequence

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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