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Plasmid#143330PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXD1 (FOXD1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)
Pooled Library#1000000048PurposeCRISPR gRNA knockout pooled library for targeting the human genomeDepositorAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
Plasmid#63166PurposeGAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporterDepositorInsert"spaghetti monster GFP-HA"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
ART
Plasmid#214063PurposeFluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ARTX_ctrl
Plasmid#214065PurposeFluorescent reporter system, NMD negative control. No intron in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-hPLD4
Plasmid#173852PurposeProtein expression plasmid for recombinant His-Myc tag human PLD4DepositorInsertPLD4 (PLD4 Human)
UseTags6xHis, Myc, Factor X cleavableExpressionMammalianMutationintracellular and transmembrane domain truncated,…PromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2.5-THP-GFP/WGA
Plasmid#80337PurposeFluorescent Reporter for Dopaminergic Neuron DifferentiationDepositorInsertrat TH promoter (Th Rat)
UseAAVTagsExpressionMutationPromoterrat Tyrosine hydroxylaseAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 hygro
Plasmid#98291PurposeVariant of lentiCRISPRv2 that confers hygromycin resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
BRD4-N CRISPR
Plasmid#140651PurposeBRD4 tagging CRISPRDepositorInsertBRD4 (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PD-L1
Plasmid#121492PurposeExpression of human PD-L1 WT in mammalian cellsDepositorInsertPD-L1 (CD274 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR]
Plasmid#86449PurposepBAc transposon with transformation marker for Tribolium transgenesis; Drives EGFP expression in the eyes; contains Gal4_ driven by Tribolium heatshock promoter; (Transformation plasmid)DepositorInsert3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pALPS env
Plasmid#101325PurposeEncodes codon optimized JR-CSF envDepositorInsertJR-CSF env
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha CAT
Plasmid#16392DepositorInsertPKG1 alpha (PRKG1 Human)
UseTagsHAExpressionMammalianMutationCAT: catalytic domain, S65DPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
Myo1E-pEGFP
Plasmid#124658PurposeMouse myosin-1e fused with EGFPDepositorInsertMyo1e (Myo1e Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEAT8-137 M1V
Plasmid#173807PurposeExpression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cellsDepositorInsertSERPiNA1 (SERPINA1 Human)
UseTagsExpressionBacterialMutationE1M Otherwise this plasmid encodes the most commo…PromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-NfiB
Plasmid#64900PurposeInducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.DepositorInsertNfiB (Nfib Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetOAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-CAG-FLEX-F14F15-BFP-(mCherry)'
Plasmid#201831PurposepiggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposaseDepositorInsertsCAG
BFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:mCD8mCherry
Plasmid#170207PurposeDrives mCD8mCherry expression with the slc1a3b promoter in astrocytesDepositorInsertmCD8
UseZebrafishTagsmCherryExpressionMutationPromoterslc1a3bAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME tdTomato-CAAX
Plasmid#135204PurposeRed fluorescent tagDepositorInserttdTomato
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GBP-mCherry-kanMX6
Plasmid#89068Purposegenomic GBP-mCherry epitope C-terminal tagging in S. pombeDepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsGBP-mCherryExpressionYeastMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only