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Plasmid#161988PurposeMammalian expression vector containing PIK3C2A tagged with GFPDepositorInsertPIK3C2A (PIK3C2A Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Enhancer Vector
Plasmid#212938PurposeFirefly luciferase vector for investigating regions controlling transcriptionDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223_HRAS_WT
Plasmid#81777PurposeGateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.DepositorInsertHRAS (HRAS Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SPHK1
Plasmid#23704DepositorInsertSPHK1 (SPHK1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pL97
Plasmid#78576PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoterDepositorTypeEmpty backboneUseStreptomyces expressionTagsExpressionBacterialMutationPromoterermEp* promoter from S. erythraeaAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJAT30
Plasmid#204294PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.DepositorInsertMHC-DsRed
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT
Plasmid#173943PurposeBacterial expression of isolated PARP1 CAT domainDepositorInsertPARP1-CAT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB016
Plasmid#99220PurposeAccessory plasmid for aaRS PACE; negative selectionDepositorInsertPT7 [SD8] gIII-neg
UseTagsExpressionBacterialMutationPromoterPT7Available sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-Cre-blast
Plasmid#179390PurposeLentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.DepositorInsertmCherry (P2A) Cre recombinase
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pdCas9-DNMT3A-PuroR
Plasmid#71667PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, S. pyogenes, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPL5606_TEF1*-Cre_ADE2
Plasmid#60932PurposeConstitutive expression vector for Cre-recombinase containing ADE2 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp2-Flag
Plasmid#11811DepositorInsertCaspase-2 (CASP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-CMV-K2M
Plasmid#21164DepositorArticleUseLentiviral; ReprogrammingTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS314-T7-GFP
Plasmid#33130DepositorInsertGFP with TDH3 5' and 3' UTR (gfp )
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
HTR1A-Tango
Plasmid#66404PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertHTR1A (HTR1A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
p281 TIR1_HA_flox STOP_mH11
Plasmid#164856PurposeCre-inducible TIR1 expression vector for mouse H11 locusDepositorInsertOsTIR1
UseTagsHA tagExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-MCM4/6
Plasmid#116951PurposeExpresses human MCM4 and human MCM6DepositorUseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDK14_IDG-K
Plasmid#158172PurposeGateway destination clone of CDK14 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDK14 (CDK14 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-SP-Cas9 reporter
Plasmid#62733PurposeMammalian CRISPR-SP-Cas9 reporterDepositorInsertCRISPR-SP-Cas9 target seq + tdTomato (out of frame)
UseCRISPR and LentiviralTagsCRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…ExpressionMammalianMutationPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only