-
Plasmid#70395PurposeGateway ORF clone of codon optimized human IRS2 [NM_003749] with stop codon (for native or N-terminal fusions)DepositorInsertIRS2 (IRS2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-L2-TALEN
Plasmid#51554PurposeExpresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.DepositorInsertH11-L2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF0706
Plasmid#143432PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOR1 (NCOR1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-R2-TALEN
Plasmid#51555PurposeExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).DepositorInsertH11-R2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-YU2gp160
Plasmid#100927PurposeMammalian expression plasmid for Env from the YU-2 HIV-1 isolateDepositorInsertHIV-1 (YU2) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-CoRESTfl
Plasmid#109156PurposeEncodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pADsacA
Plasmid#158649PurposeConstitutive transcription of sacA crRNA and GFPDepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…TagsExpressionBacterialMutationPromoterPvegAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
KU70-eGFP
Plasmid#213517PurposeExpresses human KU70-eGFP in mammalian cellsDepositorInserthuman KU70-eGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-STK11
Plasmid#23802DepositorInsertSTK11 (STK11 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
RSRF-Luc-2wt
Plasmid#31818DepositorInsertMEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
UseLuciferaseTagsExpressionMammalianMutationPromoterMEF2-dependent (-307 to -242 of Nur77/NR4A1)Available sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
-
Clomeleon
Plasmid#203723PurposeA genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentrationDepositorInsertcyan fluorescent protein linked yellow florescent protein
UseTagsExpressionBacterial and MammalianMutationCyan Fluorescent Protein, Topaz Fluorescent Prote…PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
BB44_pmc.DonorR5.TS
Plasmid#199223PurposeDonor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locusDepositorInsertExpression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLexM-SLC7A11_GFP
Plasmid#197995PurposeExpresses SLC7A11 with an N-terminal FLAG tag and C-terminal TEV-GFP-His8 tag in mammalian cellsDepositorInsertSLC7A11 (SLC7A11 Human)
UseTagsFLAG tag and TEV-GFP-His8ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcsII-LMP7-flag
Plasmid#86773PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertimmunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PGK_Prkaa1
Plasmid#204356PurposeLentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoterDepositorInsertAMP-activated protein kinase alpha 1 (Prkaa1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLXI_TRC403 SMARCB1 var 2
Plasmid#111185PurposeInducible vector (V5 tagged) with SMARCB1 variant 2DepositorInsertBAF47 (SMARCB1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterTREAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-NfiA
Plasmid#64901PurposeInducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.DepositorInsertNfia (Nfia Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2attNG-H11-long
Plasmid#51545Purposep2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus.DepositorInsertGFP4m
UseHuman targetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-SAHS1-mEGFP
Plasmid#205007PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.DepositorInsertRvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only