240,409 results

1. Mouse sgRNA library Brie in lentiCRISPRv2

   Pooled Library

   #73632

   Purpose

   Mouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pLminP_Luc2P_RE19

   Plasmid

   #90361

   Purpose

   Wnt - TCF/LEF gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Wnt

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLminP_Luc2P_RE19

   Plasmid

   #90361

   Purpose

   Wnt - TCF/LEF gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Wnt

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBluescriptII[attB/Ins1]

   Plasmid

   #74100

   Purpose

   "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Phi C31 attb element
   Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBluescriptII[attB/Ins1]

   Plasmid

   #74100

   Purpose

   "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Phi C31 attb element
   Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABEpuro-ERBB2

   Plasmid

   #40978

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBABEpuro-ERBB2

   Plasmid

   #40978

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p406TDH3

   Plasmid

   #15977

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   TDH3 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. p406TDH3

   Plasmid

   #15977

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   TDH3 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 2XMyc-LRRK2-WT

    Plasmid

    #25361

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 2XMyc-LRRK2-WT

    Plasmid

    #25361

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Ef1a-Con/Foff 2.0-BFP (AAV8)

    Viral Prep

    #137130-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-BFP (#137130). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-BFP plasmid DNA. EF1a-driven, Cre-dependent expression of BFP (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    BFP (Cre-dependent)

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pRSET FLIPPi-30m

    Plasmid

    #13556

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi T22A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    T22A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSET FLIPPi-30m

    Plasmid

    #13556

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi T22A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    T22A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBK.079

    Plasmid

    #187218

    Purpose

    Expresses Eco1 RT and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 Reverse Transcriptase
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23115 and T7/lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSET FLIPPi-200u

    Plasmid

    #13555

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi G162A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    G162A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSET FLIPPi-200u

    Plasmid

    #13555

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi G162A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    G162A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2 HRS-RFP

    Plasmid

    #29685

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2 HRS-RFP

    Plasmid

    #29685

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1_Puro_hPGK1_EBFP_Donor

    Plasmid

    #178089

    Purpose

    HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EBFP (T65S/F145Y)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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