Validated gRNA Target Sequences
The table below lists gRNA sequences that have been experimentally validated for use in CRISPR experiments. This datatable is compiled from plasmids in Addgene's database as well as from sequences provided to us by users (see below for more details). Keep in mind that additional factors must be considered before using a validated gRNA sequence in your particular experiment, so make sure to check out the particular conditions of the experiment as described in the associated article (listed below by PubMed ID). These factors include:
Does a given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and your genomic target may reduce the gRNA activity. Know your target.
Which species or variant of Cas9 (S. pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible with a single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with targets that are upstream of a 5' NGG 3' PAM sequence).
Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments use targets within promoters. When possible, the categories described on Addgene's CRISPR Plasmids and Resources page have been used to indicate the Cas9 application the gRNA was designed to accomplish.
Validated gRNA Sequence Datatable
| Target Gene | Target Species | Target Sequence | Plasmid ID | Application | Cas9 Species | PubMed ID | Depositor |
|---|---|---|---|---|---|---|---|
| OCT4 | H. sapiens | CTCCCATGCATTCAAACTG | 66989 | cut | S. pyogenes | 26028531 | Huangfu |
| OCT4 | H. sapiens | multiple, see article | 69537 | activate | S. pyogenes | 26352799 | Otonkoski |
| AAVS1 | H. sapiens | GGGGCCACTAGGGACAGGAT | 58252 | cut | S. pyogenes | 24870050 | Goncalves |
| AAVS1 | H. sapiens | GGGGCCACTAGGGACAGGAT | 41818 | cut | S. pyogenes | 23287722 | Church |
| AAVS1 | H. sapiens | GGGGCCACTAGGGACAGGAT | 50662 | cut | S. pyogenes | 24336569 | Sabatini |
| AAVS1 | H. sapiens | GGGGCCACTAGGGACAGGAT | 70661 | cut | S. pyogenes | 26472758 | Sabatini |
| AAVS1 | H. sapiens | GTCCCCTCCACCCCACAGTG | 41817 | cut | S. pyogenes | 23287722 | Church |
| actII-orf4 | S. coelicolor | ATTACCAGGGACCGGAGTTC | 62552 | cut | S. pyogenes | 25739462 | Jiang |
| ade6-L469 | S. pombe | TCTATTGTTCAGATGCCTTG | 52227 | cut | S. pyogenes | 25352017 | Zaratiegui |
| ade6-M210 | S. pombe | TCTATTGTTCAGATGCTTCG | 52226 | cut | S. pyogenes | 25352017 | Zaratiegui |
| ade6+ | S. pombe | TCTATTGTTCAGATGCCTCG | 52225 | cut | S. pyogenes | 25352017 | Zaratiegui |
| Alk and Eml | M. musculus | 64071 | cut | S. pyogenes | 25337876 | Ventura | |
| Amplicon, JAK2 | H. sapiens | GAGGCATATTCTTCTCCTGG | 70660 | cut | S. pyogenes | 26472758 | Sabatini |
| ANT1 | S. lycopersicum | TGTCGTTTATAATTTGTAGA | 70019 | cut | S. pyogenes | 26541286 | Voytas |
| APC | H. sapiens | GAAGCGGGCAAAGGGGCGAC | 58780 | cut/nick | S. pyogenes | 24954249 | Yamamoto |
| apoea | D. rerio | GGATGAGCCAAGAAGCCGCT | 42241 | cut | S. pyogenes | 23360964 | Joung |
| ASCL1 | H. sapiens | TGGATGGAGAGTTTGCAAGGAGC | 64131 | activate | S. pyogenes | 25619936 | Sato |
| ASCL1 | H. sapiens | TGGGCAGCCGCTCGCTGCAGCAG | 64130 | activate | S. pyogenes | 25619936 | Sato |
| ASCL1 | H. sapiens | TGGGGCTGGGTGTCCCATTGAAA | 64129 | activate | S. pyogenes | 25619936 | Sato |
| ASCL1 | H. sapiens | TGGTGTTTATTCAGCCGGGAGTC | 64132 | activate | S. pyogenes | 25619936 | Sato |
| Asip | R. norvegicus | ATGACAGGAGTCTAACTCAC | 60968 | cut | S. pyogenes | 24967838 | Mashimo |
| ATF1 | H. sapiens | TAGGAATCAAACACTTTTATTGG | 64690 | tag | S. pyogenes | 26355004 | Mendenhall |
| ATM | H. sapiens | TGAATTGGGATGCTGTTTTT | 58779 | cut | S. pyogenes | 24954249 | Yamamoto |
| avr-14 | C. elegans | GATTGGAGAGTTAGACCACG | 58981 | cut | S. pyogenes | 24879462 | Mello |
| avr-15 | C. elegans | GTTTGCAATATAAGTCACCC | 58982 | cut | S. pyogenes | 24879462 | Mello |
| AXIN2 | H. sapiens | TATGTTGGTGACTTGCCTCC | 58782 | cut | S. pyogenes | 24954249 | Yamamoto |
| BLIMP1 | H. sapiens | CGGATGGGGTAAACGACCCG | 59724 | cut | S. pyogenes | 25543152 | Hanna |
| BT1754 | B. thetaiotaomicron | GAAAATGGGGTGTATCCTGC | 68892 | interfere | S. pyogenes | 26918244 | Lu |
| BT1854 | B. thetaiotaomicron | ATTGAAGAACAAAAGCAGTT | 68891 | interfere | S. pyogenes | 26918244 | Lu |
| C16orf80 | H. sapiens | CGATGCTGTAGAGGATGGAG | 70650 | cut | S. pyogenes | 26472758 | Sabatini |
| C16orf80 | H. sapiens | TGTCTGAGAAGTAAACCCGT | 70651 | cut | S. pyogenes | 26472758 | Sabatini |
| C3orf17 | H. sapiens | GGGCCAAATGGGGTTTGTGG | 70653 | cut | S. pyogenes | 26472758 | Sabatini |
| C3orf17 | H. sapiens | GTGTGAGAATCCCTAAGGCG | 70652 | cut | S. pyogenes | 26472758 | Sabatini |
| C9orf114 | H. sapiens | CAGGCGGGCTCACCTCCGTG | 70654 | cut | S. pyogenes | 26472758 | Sabatini |
| C9orf114 | H. sapiens | GCGGCAGAGAAGGAGGACCG | 70655 | cut | S. pyogenes | 26472758 | Sabatini |
| CAN1 | S. cerevisiae | GATACGTTCTCTATGGAGGA | 43803 | cut | S. pyogenes | 23460208 | Church |
| CD71 | H. sapiens | GGACGCGCTAGTGTGAGTGC | 46918 | interfere | S. pyogenes | 23849981 | Qi |
| CDH1 | H. sapiens | TGACTTGCGAGGGACGCATT | 58781 | cut | S. pyogenes | 24954249 | Yamamoto |
| CDKN1B | H. sapiens | GAAGCCGGGACCTGGACCAG | 60905 | activate | S. pyogenes | 25307933 | Vale |
| CEBPB | H. sapiens | CTCCGGCCACTGCTAGCGCGG | 64036 | tag | S. pyogenes | 26355004 | Mendenhall |
| CEBPB | H. sapiens | GCCGGCAGGGGGACGCGCGCGG | 64047 | tag | S. pyogenes | 26355004 | Mendenhall |
| Cent1 | M. musculus | GGCAACGTTTGACTTCCTGA | 50718 | cut | S. pyogenes | 24284873 | Ikawa |
| CFTR | H. sapiens | TCTGTATCTATATTCATCAT | 58783 | cut | S. pyogenes | 24954249 | Yamamoto |
| CREB1 | H. sapiens | GCCACAAATCAGATTAATTTGG | 64940 | tag | S. pyogenes | 26355004 | Mendenhall |
| CREB1 | H. sapiens | GCCACAAATCAGATTAATTTGGG | 64939 | tag | S. pyogenes | 26355004 | Mendenhall |
| csr-1 | N. crassa | GAGTGGGAGGGTCCCGTCCT | 68060 | cut | S. pyogenes | Fungal Biology and Biotechnology 2015, 2:4 | Hong |
| Ctnnb1 | M. musculus | AGCTCCTTCCCTGAGTGGCA | 59912 | cut | S. pyogenes | 25119044 | Jacks |
| Ctnnb1 | M. musculus | CTGTGGTGGTGGCACCAGAA | 59911 | cut | S. pyogenes | 25119044 | Jacks |
| CXCR4 | H. sapiens | GCAGGTAGCAAAGTGACGCCGA | 46917 | interfere | S. pyogenes | 23849981 | Qi |
| CYC1m promoter | S. cerevisiae | ACAGAGCACATGCATGCCAT | 64385 | activate | S. pyogenes | 23977949 | Lu |
| CYC1m promoter | S. cerevisiae | ACTAATACTTTCAACATTTT | 64387 | activate | S. pyogenes | 23977949 | Lu |
| CYC1m promoter | S. cerevisiae | ATATCGAATTCCTGCAGCCC | 64382 | activate | S. pyogenes | 23977949 | Lu |
| CYC1m promoter | S. cerevisiae | ATATTCTTTCCTTATACATT | 64380 | activate | S. pyogenes | 23977949 | Lu |
| CYC1m promoter | S. cerevisiae | CTAGATATTAAAATGTCTAA | 64379 | activate | S. pyogenes | 23977949 | Lu |
| CYC1m promoter | S. cerevisiae | GTTGAAAGTATTAGTTAAAG | 64388 | activate | S. pyogenes | 23977949 | Lu |
| CYC1m promoter | S. cerevisiae | TACATACAGTAGGATCCTA | 64381 | activate | S. pyogenes | 23977949 | Lu |
| CYC1m promoter | S. cerevisiae | TATAGTAATTTATGCTGCAA | 64383 | activate | S. pyogenes | 23977949 | Lu |
| CYC1m promoter | S. cerevisiae | TGCAAAGGTCCTAATGTATA | 64384 | activate | S. pyogenes | 23977949 | Lu |
| CYC1m promoter | S. cerevisiae | TGTATGTACATACAGTACCC | 64386 | activate | S. pyogenes | 23977949 | Lu |
| CYC1m promoter | S. cerevisiae | TTACTGTATGTACATACAGT | 64378 | activate | S. pyogenes | 23977949 | Lu |
| ddx19 | D. rerio | GGCAACAGATTCGTGGGCCC | 47932 | cut | S. pyogenes | 23918387 | Wente |
| DDX3Y | H. sapiens | GCAGTTTAGCGATATTGACA | 70656 | cut | S. pyogenes | 26472758 | Sabatini |
| DDX3Y | H. sapiens | TCTTGTTGGGGCTAAAACCA | 70657 | cut | S. pyogenes | 26472758 | Sabatini |
| DNMT3A | H. sapiens | GAGATGATCGCCCCTTCTTC | 41822 | cut | S. pyogenes | 23287722 | Church |
| DNMT3A | H. sapiens | GCATGATGCGCGGCCCAAGG | 41821 | cut | S. pyogenes | 23287722 | Church |
| DNMT3B | H. sapiens | GAATTACTCACGCCCCAAGG | 41823 | cut | S. pyogenes | 23287722 | Church |
| dpy-10 | C. elegans | CGCTACCATAGGCACCACG | 71516 | cut | VRER Cas9 mutant | 26680661 | Fire |
| dpy-10 | C.elegans | GCTACCATAGGCACCACGAG | 65630 | cut | S. pyogenes | 26044730 | Katic |
| dpy-10 | C. elegans | GCTACCATAGGCACCACGAG | 59933 | cut | S. pyogenes | 25161212 | Fire |
| dpy-10 | C. elegans | TCCGCTACCATAGGCACCA | 71479 | cut | VQR Cas9 variant | 26680661 | Fire |
| dpy‐10 | C. elegans | GCTACCATAGGCACCACGAG | 70047 | cut | S. pyogenes | 26187122 | Seydoux |
| drd3 | D. rerio | GGAAACTACAGCCCAGCGTC | 42242 | cut | S. pyogenes | 23360964 | Joung |
| Ebf | C. intestinalis | GCTGAGGGTTGGACAACAGG | 59990 | cut | S. pyogenes | 25336740 | Christiaen |
| EGFP | A. victoria | multiple, see article | 60071 | dCas9-FokI | S. pyogenes | 24770325 | Joung |
| EGFP | A. victoria | GAAGTTCGAGGGCGACACCC | 51764 | cut | S. pyogenes | 24336571 | Zhang |
| EGFP | A. victoria | GAGCTGGACGGCGACGTAAA | 51761 | cut | S. pyogenes | 24336571 | Zhang |
| EGFP | A. victoria | GATGCCGTTCTTCTGCTTGT | 47512 | cut | S. pyogenes | 23792628 | Joung |
| EGFP | A. victoria | GGAGCGCACCATCTTCTTCA | 51763 | cut | S. pyogenes | 24336571 | Zhang |
| EGFP | A. victoria | GGCCACAAGTTCAGCGTGTC | 51762 | cut | S. pyogenes | 24336571 | Zhang |
| EGFP | A. victoria | GGCGAGGGCGATGCCACCTA | 61051 | cut | S. pyogenes | 24179142 | Del Bene |
| EGFP | A. victoria | GGGCACGGGCAGCTTGCCGG | 46760 | cut | S. pyogenes | 23918387 | Chen |
| EGFP | A. victoria | GGGCACGGGCAGCTTGCCGG | 47511 | cut | S. pyogenes | 23792628 | Joung |
| EGFP | A. victoria | GGGCGAGGAGCTGTTCACCG | 51760 | cut | S. pyogenes | 24336571 | Zhang |
| EGFP | A. victoria | GGTGAACCGCATCGAGCTGA | 51765 | cut | S. pyogenes | 24336571 | Zhang |
| EGFP | A. victoria | GGTGGTGCAGATGAACTTCA | 47513 | cut | S. pyogenes | 23792628 | Joung |
| EGFP | A. victoria | TGAAGAAGATGGTGCGCTC | 58255 | cut | S. pyogenes | 24870050 | Goncalves |
| Emx1 | H. sapiens | 42337 | cut | S. pyogenes | 23287718 | Zhang | |
| EMX1 | H. sapiens | GAGTCCGAGCAGAAGAAGAA | 47508 | cut | S. pyogenes | 23792628 | Joung |
| ETC2, TRY, and CPC | A. thaliana | 71288 | cut | S. pyogenes | 26193878 | Chen | |
| FANCF | H. sapiens | GGAATCCCTTCTGCAGCACC | 47510 | cut | S. pyogenes | 23792628 | Joung |
| fbf-2 | C. elegans | GTAGTCACGGCGATGATTA | 65597 | cut | S. pyogenes | 25249454 | Seydoux |
| fbf-2 | C. elegans | TAATCATCGCCGTGACTAC | 65591 | cut | S. pyogenes | 25249454 | Seydoux |
| fh | D. rerio | GGAGCGAGCGGAGCGGTACA | 42244 | cut | S. pyogenes | 23360964 | Joung |
| fh | D. rerio | GGAGCGGTACATGGCGACCG | 42243 | cut | S. pyogenes | 23360964 | Joung |
| GABPA | H. sapiens | GAAAAGGATAATTGAGCCCCAGG | 64254 | tag | S. pyogenes | 26355004 | Mendenhall |
| GABPA | H. sapiens | TTTGGAGTCTCAGAATGTCCTGG | 64255 | tag | S. pyogenes | 26355004 | Mendenhall |
| GAL4 UAS | GAACGACTAGTTAGGCGTGTA | 46916 | activate | S. pyogenes | 23849981 | Qi | |
| GAL4 UAS | GTTGGAGCACTGTCCTCCGAACGT | 46915 | activate | S. pyogenes | 23849981 | Qi | |
| GAL4UAS | TGGGGACAGTACTCCGCTCGAGT | 64158 | activate | S. pyogenes | 25619936 | Sato | |
| GAL4UAS | TGGGTCTTCGGAGGACAGTACTC | 64157 | activate | S. pyogenes | 25619936 | Sato | |
| GAL4UAS | TGGTCCGTCTAGAAACTCGGTAC | 64159 | activate | S. pyogenes | 25619936 | Sato | |
| gfap | D. rerio | GTGCGCAACACATAGCACCA | 65566 | cut | S. pyogenes | 25849248 | Du |
| GFP | A. victoria | GAATAGCTCAGAGGCCGAGG | 46914 | interfere | S. pyogenes | 23849981 | Qi |
| GFP | A. victoria | GGAGCGCACCATCTTCTTCA | 41820 | cut | S. pyogenes | 23287722 | Church |
| GFP | A. victoria | GTGAACCGCATCGAGCTGAA | 41819 | cut | S. pyogenes | 23287722 | Church |
| GLuc | synthetic | GATCTAGATACGACTCACTAT | 68422 | CRISPR-display | S. pyogenes | 26030444 | Rinn |
| gp78 | H. sapiens | GCCCAGCCTCCGCACCTACA | 61833 | cut | S. pyogenes | 24424410 | Ye |
| gsk3b | D. rerio | GGGACCTGACCGGCCGCAGG | 42245 | cut | S. pyogenes | 23360964 | Joung |
| his3 | S. cerevisiae | ATTGCGATCTCTTTAAAGGG | 64333 | cut | S. pyogenes | 25281382 | Jin |
| HPRT | H. sapiens | GACTGTAAGTGAATTACTT | 69539 | cut | S. pyogenes | 26130722 | Yan |
| HPRT1 | H. sapiens | CTGATAAAATCTACAGTCAT | 58778 | cut | S. pyogenes | 24954249 | Yamamoto |
| HPRT1 | H. sapiens | TTATGCTGAGGATTTGGAAA | 58771 | cut | S. pyogenes | 24954249 | Yamamoto |
| IL1RN | H. sapiens | TGGACGCAGATAAGAACCAGTT | 64142 | activate | S. pyogenes | 25619936 | Sato |
| IL1RN | H. sapiens | TGGCATCAAGTCAGCCATCAGC | 64151 | activate | S. pyogenes | 25619936 | Sato |
| IL1RN | H. sapiens | TGGGAGTCACCCTCCTGGAAAC | 64152 | activate | S. pyogenes | 25619936 | Sato |
| IL1RN | H. sapiens | TGGTGTACTCTCTGAGGTGCTC | 64140 | activate | S. pyogenes | 25619936 | Sato |
| inverted GFP | A. victoria | GAGCGGCCGCTCGAGTCTAG | 66582 | cut | S. pyogenes | 26018130 | Xue |
| inverted GFP | A. victoria | GATATCGAATTCCTGCAGCC | 66583 | cut | S. pyogenes | 26018130 | Xue |
| inverted GFP | A. victoria | GTATCGATACCGTCGACCTCG | 66581 | cut | S. pyogenes | 26018130 | Xue |
| inverted GFP | A. victoria | GTTGATCCATAACTTCGTAT | 66584 | cut | S. pyogenes | 26018130 | Xue |
| IRF1 | H. sapiens | CCGGGGGCGCTGGGCTGTCCCGG | 61079 | purify | S. pyogenes | 23942116 | Fujii |
| IRF1 | H. sapiens | GCGCTTACACTTTAGGAGACACTC | 61080 | purify | S. pyogenes | 25051498 | Fujii |
| JAK2 amplicon | H. sapiens | GGTTTAATGGAAGAGAAGGG | 70679 | cut | S. pyogenes | 26472758 | Sabatini |
| K08F4.2 | C. elegans | AATCACTCCCTGTTTGTGT | 66085 | cut | S. pyogenes | 25249454 | Seydoux |
| K08F4.2 | C. elegans | CACGAGGTGGTATGCGCAG | 66095 | cut | S. pyogenes | 25249454 | Seydoux |
| K08F4.2 | C. elegans | CGCAGCGGTTTCCAAAATG | 66092 | cut | S. pyogenes | 25249454 | Seydoux |
| K08F4.2 | C. elegans | GCCTTAACCCAGAATAAGA | 66087 | cut | S. pyogenes | 25249454 | Seydoux |
| K08F4.2 | C. elegans | TATTAAATGCAGATAACCT | 66089 | cut | S. pyogenes | 25249454 | Seydoux |
| KANK3 | H. sapiens | GCATGGGTGATGTCAATGCC | 69238 | cut | S. pyogenes | 26480473 | Wolfe |
| Kit-1 | R. norvegicus | CATCTGTGCGGCCGTTGGCT | 60969 | cut | S. pyogenes | 24967838 | Mashimo |
| Kit-2 | R. norvegicus | CTAACGTTCCAGCGCTCGTT | 60970 | cut | S. pyogenes | 24967838 | Mashimo |
| Kit-2 | R. norvegicus | GTCAAGATGTCATCTTACGG | 60971 | cut | S. pyogenes | 24967838 | Mashimo |
| klp-12 | C. elegans | GATCCACAAGTTACAATTGG | 46170 | cut | S. pyogenes | 23817069 | Calarco |
| Kras, p53, and Lkb1 | M. musculus | multiple, see article | 60224 | cut | S. pyogenes | 25263330 | Zhang |
| LacZ | E. coli | TGCGAATACGCCCACGCGAT | 60225 | cut | S. pyogenes | 25263330 | Zhang |
| lC.GA4a | B. oleracea | GTTGAGAGGGGAGCCGGTGA | 68256 | cut | S. pyogenes | 26616834 | Patron |
| lC.GA4a | B. oleracea | GTTTTCACTTGCGGCCGGAG | 68255 | cut | S. pyogenes | 26616834 | Patron |
| leu2 | S. cerevisiae | AATCACAGCCGAAGCCATTA | 64332 | cut | S. pyogenes | 25281382 | Jin |
| Lkb1 | M. musculus | GTGGTGGGCCGCAGTCACAA | 66894 | cut | S. pyogenes | 26178787 | Winslow |
| Lox-STOP-Lox | synthetic | GCGTATAGCATACATTATACG | 66586 | cut | S. pyogenes | 26018130 | Xue |
| Lox-STOP-Lox | synthetic | GCGTATAGTACACATTATACG | 66585 | cut | S. pyogenes | 26018130 | Xue |
| LRTM2 | H. sapiens | GCTGGCGGAAGACAGAGTGC | 69237 | cut | S. pyogenes | 26480473 | Wolfe |
| LYP1 | S. cerevisiae | CATAATAACGTCCAATAAAT | 60847 | cut | S. pyogenes | 25139909 | Cate |
| Mettl14 | M. musculus | GCCGCTCCCGGATCTCCTGC | 61514 | nick | S. pyogenes | 25569111 | Hanna |
| Mettl14 | M. musculus | GCGGCAGCTCCTAGCTCAGC | 61515 | nick | S. pyogenes | 25569111 | Hanna |
| mitf | S. scrofa | CTTTCGGATATAATCCACGG | 69801 | cut | S. pyogenes | 26293209 | Zhao |
| mitfa | D. rerio | GGTCTCTCGCAGGATGTTGC | 47931 | cut | S. pyogenes | 23918387 | Chen |
| mKate | synthetic | ATGAGAATCAAGGCGGTCGA | 62715 | cut | S. pyogenes | 25527740 | Bleris |
| MUC4 | H. sapiens | GTGGCGTGACCTGTGGATGCTG | 51025 | visualize | S. pyogenes | 24360272 | Qi |
| MYOD1 | H. sapiens | TGGCCTCCCTCCCTGCCCGGTAG | 64138 | activate | S. pyogenes | 25619936 | Sato |
| MYOD1 | H. sapiens | TGGGAGGTTTGGAAAGGGCGTGC | 64139 | activate | S. pyogenes | 25619936 | Sato |
| MYOD1 | H. sapiens | TGGGCCTGGGCTCCGGGGCGTTT | 64136 | activate | S. pyogenes | 25619936 | Sato |
| MYOD1 | H. sapiens | TGGGGGCCCCTGCGGCCACCCCG | 64137 | activate | S. pyogenes | 25619936 | Sato |
| NANOG | H. sapiens | TGGCATATTCCTGATTTAAAAGT | 64156 | activate | S. pyogenes | 25619936 | Sato |
| NANOG | H. sapiens | TGGCGCCAGGAGGGGTGGGTCTA | 64153 | activate | S. pyogenes | 25619936 | Sato |
| NANOG | H. sapiens | TGGGCCTTGGTGAGACTGGTAGA | 64154 | activate | S. pyogenes | 25619936 | Sato |
| NANOG | H. sapiens | TGGTGTCTTCAGGTTCTGTTGCT | 64155 | activate | S. pyogenes | 25619936 | Sato |
| NanoLuc | synthetic | AGCTTACGCCACCCTGTTCC | 68897 | interfere | S. pyogenes | 26918244 | Lu |
| NanoLuc | synthetic | GACAGAACGATGCGCTGAAT | 68898 | interfere | S. pyogenes | 26918244 | Lu |
| NanoLuc | synthetic | TCACGCTCACACCCAGGTTC | 68895 | interfere | S. pyogenes | 26918244 | Lu |
| NanoLuc | synthetic | TTGATCCAAATTATAACCCG | 68896 | interfere | S. pyogenes | 26918244 | Lu |
| NDM-1 | GGGCAGTCGCTTCCAACGGTTTGATCGTCA | 61270 | cut | S. pyogenes | 25240928 | Lu | |
| negative control | CTGGAATGAATTGGCCTATG | 68893 | interfere | S. pyogenes | 26918244 | Lu | |
| negative control | M. musculus | GCGAGGTATTCGGCTCCGCG | 66895 | cut | S. pyogenes | 26178787 | Winslow |
| negative control | C. intestinalis | GCTTTGCTACGATCTACATT | 60006 | cut | S. pyogenes | 25336740 | Christiaen |
| negative control | synthetic | GTCAAGGCACTCTTGCCTA | 64955 | cut | S. pyogenes | 25527740 | Bleris |
| negative control | H. sapiens | GTTCCGCGTTACATAACTTA | 50927 | S. pyogenes | 24346702 | Wolfe | |
| Neomycin | TCATGGCTGATGCAATGCGG | 67594 | cut | S. pyogenes | 26178787 | Winslow | |
| NeuN | M. musculus | TCCGGTTCAGGGACCCCGAC | 60227 | cut | S. pyogenes | 25263330 | Zhang |
| Neurog2 | H. sapiens | GTCTCTATCACTGATAGGGA | 64161 | activate | S. pyogenes | 25619936 | Sato |
| Neurog2 | H. sapiens | GTGAATGATGATAATACGAT | 64160 | activate | S. pyogenes | 25619936 | Sato |
| Oct4A (POU5F1) | H. sapiens | GGGGCGCCAGTTGTGTCTCC | 50922 | interfere | S. pyogenes | 24346702 | Wolfe |
| Oct4A (POU5F1) | H. sapiens | GTGGGACTGGGGAGGGAGAG | 50921 | interfere | S. pyogenes | 24346702 | Wolfe |
| Pal1 | synthetic | GCTCTGTGACTAGTCACAGAG | 71483 | cut | S. pyogenes | 26527385 | Sherwood |
| Pal7 | synthetic | GGCTTAGTACTAGTACTAAGC | 71484 | cut | S. pyogenes | 26527385 | Sherwood |
| PAX6 | H. sapiens | GATACTGGAGAAGCAGGGGC | 68466 | cut | S. pyogenes | 26145478 | Zhang |
| PAX6 | H. sapiens | GTAGATTTTGTATGCACTGC | 68465 | cut | S. pyogenes | 26145478 | Zhang |
| PcfiA | AAACAAAACCTCATCAGGCA | 68900 | interfere | S. pyogenes | 26918244 | Lu | |
| PcfiA | GAAGCTCACTCCTTAGCACG | 68899 | interfere | S. pyogenes | 26918244 | Lu | |
| PDS | N. benthamiana | GCCGTTAATTTGAGAGTCCA | 46966 | cut | S. pyogenes | 23929340 | Kamoun |
| PDS1 | N. benthamiana | multiple, see article | 49772 | cut | S. pyogenes | 24112467 | Kamoun |
| PDS3 | A. thaliana | GGACTTTTGCCAGCCATGGT | 52255 | cut | S. pyogenes | 23929339 | Sheen |
| pGL3-Basic-8x-gRNA-eGFP reporter | synthetic | AAAGGTCGAGAAACTGCAAA | 60719 | activate | S. pyogenes | 25664691 | Gersbach |
| pha-1 | C. elegans | ATGAATAACTTGATGAACAT | 61252 | cut | S. pyogenes | 25491644 | Ward |
| PLXNB2 | H. sapiens | GGGCCCAACCTAGGGCATGG | 69240 | cut | S. pyogenes | 26480473 | Wolfe |
| PM19 | H. vulgare | GCTCTCCACTCTGGGCTCTT | 68253 | cut | S. pyogenes | 26616834 | Patron |
| PM19 | H. vulgare | GGCTGGCGTTGGTCGTAACA | 68254 | cut | S. pyogenes | 26616834 | Patron |
| Prnp | M. musculus | GGTGGAACACCGGTGGAAGC | 61857 | cut | S. pyogenes | 25490046 | Schmitt-Ulms |
| Prnp | M. musculus | TTGGCCCCATCCACCGCCAT | 61856 | cut | S. pyogenes | 25490046 | Schmitt-Ulms |
| Pten | M. musculus | AGATCGTTAGCAGAAACAAA | 59909 | cut | S. pyogenes | 25119044 | Jacks |
| Pten | M. musculus | GCTGTAGTAATATCTGCTAT | 66588 | cut | S. pyogenes | 26018130 | Xue |
| Pten | M. musculus | GTTTCATAGCGGCCACGAAGT | 66587 | cut | S. pyogenes | 26018130 | Xue |
| RAD21 | H. sapiens | CCAAGGTTCCATATTATATAAGG | 64057 | tag | S. pyogenes | 26355004 | Mendenhall |
| rde-1(D718) | C. elegans | TGCCATTAACTATGTATGT | 59927 | cut | S. pyogenes | 25161212 | Fire |
| rde-1(D801) | C. elegans | GATATTGTAGTCTATCGAGA | 59928 | cut | S. pyogenes | 25161212 | Fire |
| rde-1(H974) | C. elegans | GATAAATGAGCATAATGAAC | 59929 | cut | S. pyogenes | 25161212 | Fire |
| rgs4 | D. rerio | GGAGAAGGTGAAGGACACTG | 42246 | cut | S. pyogenes | 23360964 | Joung |
| RNF2 | H. sapiens | GTCATCTTAGTCATTACCTG | 47509 | cut | S. pyogenes | 23792628 | Joung |
| rol-6(su1006) | C. elegans | GTGAGACGTCAACAATATGG | 59930 | cut | S. pyogenes | 25161212 | Fire |
| Rosa26 | M.musculus | ACTCCAGTCTTTCTAGAAGA | 64216 | cut | S. pyogenes | 25803306 | Kuhn |
| rpsL | E. coli | AAAAAACCGAACTCCGCGCTGCGTAAAGTA | 44505 | cut | S. pyogenes | 23360965 | Marraffini |
| scramble | synthetic | AACCCCTGATTGTATCCGCA | 62285 | interfere | S. pyogenes | 25422271 | Voigt |
| slc24a5 (golden) | D. rerio | GGTCTCTCGCAGGATGTTGC | 47930 | cut | S. pyogenes | 23918387 | Chen |
| SOX17 | H. sapiens | GCCCAGCCCGGCCATCACCG | 59725 | cut | S. pyogenes | 25543152 | Hanna |
| Sox17 | H. sapiens | GCTCCGGCTAGTTTTCCCGG | 50925 | activate | S. pyogenes | 24346702 | Wolfe |
| Sox17 | H. sapiens | GGAGGGGCAAGGGGCGGGCG | 50924 | activate | S. pyogenes | 24346702 | Wolfe |
| Sox17 | H. sapiens | GGGCAAGTACGTCGATTCCA | 50926 | activate | S. pyogenes | 24346702 | Wolfe |
| Sox17 | H. sapiens | GGGCGTGGGCCTAACGACGC | 50923 | activate | S. pyogenes | 24346702 | Wolfe |
| sqt-1 | C. elegans | GGAAGGACATAGTTGTCAT | 59935 | cut | S. pyogenes | 25161212 | Fire |
| sqt-1 | C. elegans | TGTGGAGTTGGGGTAGCGT | 60212 | cut | S. pyogenes | 25161212 | Fire |
| sv40 promoter | synthetic | CATACTTCTGCCTGCTGGGGAGCCTG | 62338 | activate/interfere | S. pyogenes | 25533786 | Qi |
| SV40.NT1 | H. sapiens | GAATAGCTCAGAGGCCGAGG | 62340 | scaffold | S. pyogenes | 25533786 | Qi & Lim |
| swan-2 | C. elegans | ACAAATTGATATCCAATCA | 66100 | cut | S. pyogenes | 25249454 | Seydoux |
| swan-2 | C. elegans | TGAAGAAAGTTATACTCGA | 66099 | cut | S. pyogenes | 25249454 | Seydoux |
| T (BRACHYURY) | H. sapiens | CACGCGCAGTTCGCGCTCTG | 59726 | cut | S. pyogenes | 25543152 | Hanna |
| TEF | S. cerevisiae | TTGATATTTAAGTTAATAAA | 62320 | scaffold | S. pyogenes | 25533786 | Qi & Lim |
| TEF1 | S. cerevisiae | TTGATATTTAAGTTAATAAA | 46922 | interfere | S. pyogenes | 23849981 | Weissman |
| telomeres | H. sapiens | GTTAGGGTTAGGGTTAGGGTTA | 51024 | visualize | S. pyogenes | 24360272 | Qi |
| TET | S. cerevisiae | ACTTTTCTCTATCACTGATA | 62313 | scaffold | S. pyogenes | 25533786 | Qi & Lim |
| TET promoter | TATCAGTGATAGAGAAAAGT | 46923 | interfere | S. pyogenes | 23849981 | Weissman | |
| Tet3G | synthetic | GTACGTTCTCTATCACTGATA | 62327 | activate/interfere | S. pyogenes | 25533786 | Qi |
| TGM2 | H. sapiens | CCTGGACCCAACGCCCCAGG | 69239 | cut | S. pyogenes | 26480473 | Wolfe |
| TGME49_290860 | T. gondii | ATTGGTCTGACAGCGATGTG | 59855 | cut | S. pyogenes | 25480939 | Sibley |
| th | D. rerio | GGATGCGCGTAAGGAGCGCG | 42247 | cut | S. pyogenes | 23360964 | Joung |
| th | D. rerio | GGGTTGTTACACTCATAAAG | 65565 | cut | S. pyogenes | 25849248 | Du |
| tia1l | D. rerio | GGTATGTCGGGAACCTCTCC | 42248 | cut | S. pyogenes | 23360964 | Joung |
| tph1a | D. rerio | GGGAAAACACAACCGCAGCC | 42249 | cut | S. pyogenes | 23360964 | Joung |
| TRE3G | H. sapiens | GTACGTTCTCTATCACTGATA | 62325 | scaffold | S. pyogenes | 25533786 | Qi & Lim |
| trp1 | S. cerevisiae | GTCAATTGTTCTCTTTCTAT | 64331 | cut | S. pyogenes | 25281382 | Jin |
| Trp53 | M. musculus | CCTCGAGCTCCCTCTGAGCC | 59910 | cut | S. pyogenes | 25119044 | Jacks |
| TS2 | H. sapiens | GACCCCCTCCACCCCGCCTC | 69234 | cut | S. pyogenes | 26480473 | Wolfe |
| TS3 | H. sapiens | GGTGAGTGAGTGTGTGCGTG | 69235 | cut | S. pyogenes | 26480473 | Wolfe |
| TS4 | H. sapiens | GAGTCCGAGCAGAAGAAGAA | 69236 | cut | S. pyogenes | 26480473 | Wolfe |
| ttTi5605 Mos1 insertion site | C. elegans | ATATCAGTCTGTTTCGTAA | 47550 | cut | S. pyogenes | 23995389 | Goldstein |
| tyr | D. rerio | CCCCAGAAGTCCTCCAGTCC | 46761 | cut | S. pyogenes | 23918387 | Chen |
| tyr | D. rerio | GGACTGGAGGACTTCTGGGG | 64250 | cut | S. pyogenes | 25855067 | Chen |
| Tyr (albino) | R. norvegicus | TTTCCAGGATTATGTAATAG | 60966 | cut | S. pyogenes | 24967838 | Mashimo |
| Tyr (wild type) | R. norvegicus | TTTCCAGGATTACGTAATAG | 60967 | cut | S. pyogenes | 24967838 | Mashimo |
| unc-109(n499) | C. elegans | GGAACTCGTGTCAAAACAAC | 59932 | cut | S. pyogenes | 25161212 | Fire |
| unc-119 | C. elegans | GAATTTTCTGAAATTAAAGA | 46169 | cut | S. pyogenes | 23817069 | Calarco |
| unc-22 | C. elegans | GAACCCGTTGCCGAATACAC | 58202 | cut | S. pyogenes | 24879462 | Mello |
| unc-58(e665) | C. elegans | TCCACGCACATGGTCACTA | 59931 | cut | S. pyogenes | 25161212 | Fire |
| ura3 | S. cerevisiae | GAGTAAAAAATTGTACTTGG | 64330 | cut | S. pyogenes | 25281382 | Jin |
| USP13 | H. sapiens | GCCGCCCGGCATGCCGAACA | 61812 | cut | S. pyogenes | 24424410 | Ye |
| VEGF | H. sapiens | GACCCCCTCCACCCCGCCTC | 47506 | cut | S. pyogenes | 23792628 | Joung |
| VEGF | H. sapiens | GGGTGGGGGGAGTTTGCTCC | 47505 | cut | S. pyogenes | 23792628 | Joung |
| VEGF | H. sapiens | GGTGAGTGAGTGTGTGCGTG | 47507 | cut | S. pyogenes | 23792628 | Joung |
| VR | synthetic | TGCGCTCGGTCGTTCGGCTG | 62286 | interfere | S. pyogenes | 25422271 | Voigt |
| Y61A9LA.1 | C. elegans | GGATGGATGTGTAGTCAATT | 61250 | cut | S. pyogenes | 25491644 | Ward |
| yellow | D. melanogaster | GGTTTTGGACACTGGAACCG | 49331 | cut | S. pyogenes | 24326186 | Liu |
| near LoxP sites | synthetic | CGAAGTTATATTAAGGGTTC | 69992 | cut | S. pyogenes | 25155555 | Cepko |
| ANT1 | S. lycopersicum | ACAATTTAATACACCTTTT | 70018 | cut | S. pyogenes | 26541286 | Voytas |
| BCR/ABL amplicon | H. sapiens | GGCTCCCTTCAAGTGGGATG | 70658 | cut | S. pyogenes | 26472758 | Sabatini |
| BACH2 | H. sapiens | AATGTAGCGATTGAGAGTGTGGG | 71828 | methylation | S. pyogenes | 26969735 | Zoldoš |
| Non-targeting | H. sapiens | GTAGGCGCGCCGCTCTCTAC | 71830 | methylation | S. pyogenes | 26969735 | Zoldoš |
| AAVS1 | H. sapiens | GGGGCCACTAGGGACAGGAT | 72833 | cut | S. pyogenes | 27052166 | Kanemaki |
| mmp21 | D. rerio | CGGAGCTGATCACTGACA | 72890 | cut | S. pyogenes | 26429889 | Katsanis |
| GFPmut3b | A. victoria | ACCATCTAATTCAACAAGAATT | 73221 | interfere | S. pyogenes | 26689101 | Hudson |
| pgi | C. glutamicum | TGACCGATCATTACTCAAACTTCC | 74066 | interfere | S. pyogenes | 26829286 | Lu |
| pgi | C. glutamicum | TTGCCTGGAAGTTTGAGTAATGAT | 74067 | interfere | S. pyogenes | 26829286 | Lu |
| pck | C. glutamicum | AGGGCGAGGCGCCGACCAAGAATA | 74068 | interfere | S. pyogenes | 26829286 | Lu |
| pck | C. glutamicum | TCCAGTTCAGCAGTTCCTTATTCT | 74069 | interfere | S. pyogenes | 26829286 | Lu |
| pyk | C. glutamicum | AGATTGTATGTACCCTAGGCCCAG | 74070 | interfere | S. pyogenes | 26829286 | Lu |
| pyk | C. glutamicum | ATTCCATCTGCACTAGCCACCGCT | 74072 | interfere | S. pyogenes | 26829286 | Lu |
| rfp | AAGTTCGTATGGAAGGTTCCGTTAA | 74075 | interfere | S. pyogenes | 26829286 | Lu | |
| LacZ | E.coli | CCCGAATCTCTATCGTGCGG | 74179 | cut | S. pyogenes | 26627737 | Moffat |
| PSMD1 | H. sapiens | TGTGCGCTACGGAGCTGCAA | 74180 | cut | S. pyogenes | 26627737 | Moffat |
| PSMD1 | H. sapiens | ACCAGAGCCACAATAAGCCA | 74181 | cut | S. pyogenes | 26627737 | Moffat |
| PSMB2 | H. sapiens | ATGTTCTTGTCGCCTCCGAC | 74184 | cut | S. pyogenes | 26627737 | Moffat |
| PSMB2 | H. sapiens | AATATTGTCCAGATGAAGGA | 74185 | cut | S. pyogenes | 26627737 | Moffat |
| EIF3D | H. sapiens | TGTAGGTTGCCTCCATGGCC | 74187 | cut | S. pyogenes | 26627737 | Moffat |
| EIF3D | H. sapiens | AGACGACCCTGTCATCCGCA | 74188 | cut | S. pyogenes | 26627737 | Moffat |
| Luciferase | ACAACTTTACCGACCGCGCC | 74190 | cut | S. pyogenes | 26627737 | Moffat | |
| AMPK alpha 1 | H. sapiens | GGCTGTCGCCATCTTTCTCC | 74374 | nick | S. pyogenes | 26816379 | Shaw |
| AMPK alpha 1 | H. sapiens | GAAGATCGGCCACTACATTC | 74375 | nick | S. pyogenes | 26816379 | Shaw |
| AMPK alpha 2 | H. sapiens | GTCAGCCATCTTCGGCGCGCG | 74376 | nick | S. pyogenes | 26816379 | Shaw |
| AMPK alpha 2 | H. sapiens | GAAGATCGGACACTACGTGC | 74377 | nick | S. pyogenes | 26816379 | Shaw |
| ACTB | H. sapiens | UGGAGCGAGCAUCCCCCAAA | 74705 | RNA targeting | S. pyogenes | 26997482 | Yeo |
| TFRC | H. sapiens | GCACUGACCAGAUAAGAAUG | 74709 | RNA targeting | S. pyogenes | 26997482 | Yeo |
| AAVS1 | H. sapiens | TGTCCCTAGTGGCCCCACTG | cut | S. pyogenes | 26789497 | Corn | |
| AAVS1 | H. sapiens | ACAGTGGGGCCACTAGGGAC | cut | S. pyogenes | 26789497 | Corn | |
| BFP | ATGGCGTGCAGTGCTTCAGC | cut | S. pyogenes | 26789497 | Corn | ||
| EMX1 | H. sapiens | CGATGTCACCTCCAATGACT | cut | S. pyogenes | 26789497 | Corn | |
| CCR5 | H. sapiens | TGACATCAATTATTATACAT | cut | S. pyogenes | 26789497 | Corn | |
| CXCR4 | H. sapiens | CCTCTTTGTCATCACGCTTC | cut | S. pyogenes | 26789497 | Corn | |
| AOC | N. attenuata | CAAAAGACTGTCAATTCCCT | cut | S. pyogenes | 26479191 | Kim | |
| PHYB | A. thaliana | CACTAGGAGCAACACCCAA | cut | S. pyogenes | 26479191 | Kim | |
| P450 | O. sativa | CATATAGTTGGGTCATGGCA | cut | S. pyogenes | 26479191 | Kim | |
| DWD1 | O. sativa | TGCATCGTCCAAGCGCACAG | cut | S. pyogenes | 26479191 | Kim | |
| DWD1 | O. sativa | CTACGACGTCAGGTTCTACC | cut | S. pyogenes | 26479191 | Kim | |
| BRI1 | A. thaliana | TTTGAAAGATGGAAGCGCGG | cut | S. pyogenes | 26479191 | Kim | |
| BRI1 | A. thaliana | TGAAACTAAACTGGTCCACA | cut | S. pyogenes | 26479191 | Kim | |
| BIN2 | L. sativa | ATCACAGTGATGCTCGTCAA | cut | S. pyogenes | 26479191 | Kim | |
| tdtomato | Synthetic | CGAAATGAGAAAGGGAGCTACAAC | 47869 | cut | N. meningitidis | 23940360 | Thomson |
| OCT4 | H. sapiens | GTTGTAGCTCCCTTTCTCATTTCG | 47870 | cut | N. meningitidis | 23940360 | Thomson |
| Protospacer A | Synthetic | TACCATCTCAAGCTTGTTGA | 48651 | cut | N. meningitidis | 24076762 | Church |
| Protospacer B | Synthetic | ACTTTAAAAGTATTCGCCAT | 48652 | cut | N. meningitidis | 24076762 | Church |
| Protospacer A | Synthetic | TACCATCTCAAGCTTGTTGA | 48653 | cut | S. thermophilus | 24076762 | Church |
| Protospacer B | Synthetic | ACTTTAAAAGTATTCGCCAT | 48654 | cut | S. thermophilus | 24076762 | Church |
| Protospacer A | Synthetic | TACCATCTCAAGCTTGTTGA | 48655 | cut | T. denticola | 24076762 | Church |
| Protospacer B | Synthetic | ACTTTAAAAGTATTCGCCAT | 48656 | cut | T. denticola | 24076762 | Church |
| UPRT | Toxoplasma gondii | GGCGTCTCGATTGTGAGAGC | 54467 | cut | S. pyogenes | 24825012 | Sibley |
| GFP | Synthetic | gRNA1: GAGCTGGACGGCGACGTAAA; gRNA2: CAGAACACCCCCATCGGCGA | 72619 | cut | S. pyogenes | 26493208 | Guigo, Johnson |
| Malat1 | H. sapiens | gRNA1: GAACCGGTGGGGCTGCGTCA; gRNA2: GGCAGGAGAGGCCAGTTGCG | 72620 | cut | S. pyogenes | 26493208 | Guigo, Johnson |
| Malat1 | H. sapiens | gRNA1: GCAACTTCCATTTTCAGTCT; gRNA2: GGAAGCCTCAGCTCGCCTGA | 72621 | cut | S. pyogenes | 26493208 | Guigo, Johnson |
| Malat1 | H. sapiens | gRNA1:GCTGGGGCTCAGTTGCGTAA; gRNA2:AGGTTTCTAAAACATGACGG | 72622 | cut | S. pyogenes | 26493208 | Guigo, Johnson |
| Malat1 | H. sapiens | gRNA1:GTTGAGATGAAGCTTCTTCA; gRNA2:TCAACCGTCCCTGCAAGGCT | 72623 | cut | S. pyogenes | 26493208 | Guigo, Johnson |
| Malat1 | H. sapiens | gRNA1: GAAACCTCGTGTAGCTATCA; gRNA2: AATGTGAAGGACTTTCGTAA | 72624 | cut | S. pyogenes | 26493208 | Guigo, Johnson |
| TFRC | H. sapiens | gRNA1:GCAGCCTCAGAAATACAAAA, gRNA2CGGGATATCGGGTGGCGGCT | 72626 | cut | S. pyogenes | 26493208 | Guigo, Johnson |
| TCRC | H. sapiens | gRNA1:GGGGAGCGGGAAAGCGGTCG; gRNA2:AACTGACCTTCAGGCCCGTA | 72627 | cut | S. pyogenes | 26493208 | Guigo, Johnson |
| UCA1 | H. sapiens | gRNA1:GGTTTCCTTTTAGATGACGG; gRNA2:TCTGAAAAGAGAGTCAGCGA | 72628 | cut | S. pyogenes | 26493208 | Guigo, Johnson |
| GFPmut3b | synthetic | ACCATCTAATTCAACAAGAATT | 73224 | interfere | S. pyogenes | 26689101 | Hudson |
| xylR | C. beijerinckii | CGAGTTAGACATAATAGTGA | 73228 | nick | S. pyogenes | 27213844 | Yang |
| RIPK1 | H. sapiens | GCTCTGCTGGGAAGCGAATC | 75163 | cut | S. pyogenes | 27194728 | Bornhauser |
| Caspase-8 | H. sapiens | GCCTGGACTACATTCCGCAA | 75164 | cut | S. pyogenes | 27194728 | Bornhauser |
| RIP3 | H. sapiens | TACACGAGTGATGGTTCGGT | 75165 | cut | S. pyogenes | 27194728 | Bornhauser |
| FADD | H. sapiens | ACTCCCGCACACGCTCTGTC | 75166 | cut | S. pyogenes | 27194728 | Bornhauser |
| MLKL | H. sapiens | ATCCCCGTGGATTCTGCTAA | 75167 | cut | S. pyogenes | 27194728 | Bornhauser |
| Prkaa1 | M. musculus | TTATTGTCACAGGCATATGG | 79004 | cut | S. pyogenes | 26944679 | Shaw |
| Prkaa2 | M. musculus | ACAGGCATATGGTTGTCCAT | 79005 | cut | S. pyogenes | 26944679 | Shaw |
| Tfeb | M. musculus | AGCACTGTTGCCGGCCGAGG | 79006 | cut | S. pyogenes | 26944679 | Shaw |
Please refer to the primary article to confirm details and outcomes.
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Validated gRNA sequences can be added to this table from any peer reviewed publication. To add validated gRNA sequences to our datatable, please download the following spreadsheet, fill out as much information as possible on the sequences, and email the file to [email protected] with the subject heading "gRNA sequence spreadsheet". Thanks for helping us expand and improve our resources!
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