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Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology.

Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
163043pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)QuasAr2-mOrange2 voltage sensor (plasma membrane)
163044pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)CheRiff-eGFP channelrhodopsin (plasma membrane)
163045pMOS008: GCaMP6F calcium sensor (cytosolic)GCaMP6F calcium sensor (cytosolic)
163046pMOS028: Mitycam calcium sensor (mitochondrial)Mitycam calcium sensor (mitochondrial)
163047pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)GCaMPer calcium sensor (endoplasmic reticulum)
163048pMOS019: ClopHensorN chloride sensor (cytosolic)ClopHensorN chloride sensor (cytosolic)
163049pMOS005: superecliptic pHluorin (cytosolic)superecliptic pHluorin (cytosolic)
163050pMOS011: superecliptic pHluorin (mitochondrial)superecliptic pHluorin (mitochondrial)
163051pMOS031: superecliptic pHluorin (endoplasmic reticulum)superecliptic pHluorin (endoplasmic reticulum)
163052pMOS017: ratiometric pHluorin (cytosolic)ratiometric pHluorin (cytosolic)
163053pMOS018: ratiometric pHluorin (mitchondrial)ratiometric pHluorin (mitchondrial)
163054pMOS030: ratiometric pHluorin (endoplasmic reticulum)ratiometric pHluorin (endoplasmic reticulum)
163055pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)Grx1-roGFP2 glutathione redox sensor (cytosolic)
163056pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)
163057pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)
163058pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)
163059pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)
163060pMOS023: Peredox NADH/NAD+ sensor (cytosolic)Peredox NADH/NAD+ sensor (cytosolic)
163061pMOS007: PercevalHR ATP/ADP sensor (cytosolic)PercevalHR ATP/ADP sensor (cytosolic)
163062pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)
163063pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)
163064pMOS008E: Entry vector for: GCaMP6F calcium sensor (cytosolic)Entry vector for: GCaMP6F calcium sensor (cytosolic)
163065pMOS028E: Entry vector for: Mitycam calcium sensor (mitochondrial)Entry vector for: Mitycam calcium sensor (mitochondrial)
163066pMOS019E: Entry vector for: ClopHensorN chloride sensor (cytosolic)Entry vector for: ClopHensorN chloride sensor (cytosolic)
163067pMOS011E: Entry vector for: superecliptic pHluorin (mitochondrial)Entry vector for: superecliptic pHluorin (mitochondrial)
163068pMOS031E: Entry vector for: superecliptic pHluorin (endoplasmic reticulum)Entry vector for: superecliptic pHluorin (endoplasmic reticulum)
163069pMOS017E: Entry vector for: ratiometric pHluorin (cytosolic)Entry vector for: ratiometric pHluorin (cytosolic)
163070pMOS018E: Entry vector for: ratiometric pHluorin (mitchondrial)Entry vector for: ratiometric pHluorin (mitchondrial)
163071pMOS030E: Entry vector for: ratiometric pHluorin (endoplasmic reticulum)Entry vector for: ratiometric pHluorin (endoplasmic reticulum)
163072pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)
163073pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)
163074pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)
163075pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)
163076pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)
163077pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Antibodies from Article