pMOS030: ratiometric pHluorin (endoplasmic reticulum)
(Plasmid
#163054)
-
Purposeratiometric pHluorin (endoplasmic reticulum)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 163054 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLX304
- Backbone size w/o insert (bp) 9377
-
Vector typeMammalian Expression, Lentiviral
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameratiometric pHluorin
-
SpeciesSynthetic
-
Insert Size (bp)780
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byGero Miesenboeck
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see PMID: 9671304 for original reporter gene characterization.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMOS030: ratiometric pHluorin (endoplasmic reticulum) was a gift from Adam Cohen (Addgene plasmid # 163054 ; http://n2t.net/addgene:163054 ; RRID:Addgene_163054) -
For your References section:
Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE. Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5. 10.1038/s41467-020-17607-5 PubMed 32753572