pMOS028E: Entry vector for: Mitycam calcium sensor (mitochondrial)
(Plasmid
#163065)
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PurposeEntry vector for: Mitycam calcium sensor (mitochondrial)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163065 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR
- Backbone size w/o insert (bp) 2586
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Vector typeGateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMitycam
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SpeciesSynthetic
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Insert Size (bp)1392
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F(-21)
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySarah Kettlewell and Godfrey Smith
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see PMID: 19249308 for original reporter gene characterization.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMOS028E: Entry vector for: Mitycam calcium sensor (mitochondrial) was a gift from Adam Cohen (Addgene plasmid # 163065 ; http://n2t.net/addgene:163065 ; RRID:Addgene_163065) -
For your References section:
Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE. Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5. 10.1038/s41467-020-17607-5 PubMed 32753572