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  2. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...isolation of cells, organelles, lipoproteins, and macromolecules. Importantly, iodixanol is inert and non-toxic...NaCl to destabilize ionic interactions between macromolecules. The 40% and 25% steps are used to remove contaminants...option #1. Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua...

  3. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...2 O 0.2 μL Taq DNA Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design...added to a master mix. Place reaction tubes in PCR machine. Set annealing temperature 5°C below the primer...liquid to the bottom before placing it in the PCR machine. Diluting Primers Most people order primers from...

  5. DNA Quantification

    Type
    Protocol
    ...Blanking' measures the background inherent to the machine and your solvent. If using a NanoDrop to measure...
Showing: 1 - 5 of 5 results