...bias observed in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed...primary cells Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific...rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human...2023). They found the best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier...to identification and characterization of AAV.CAP-Mac. Image reproduced from Chuapoco, Flytzanis, Goeden...2023, under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco, M.R., Flytzanis, N.C.,...

... our paper, “Synthetic recombinase-based state machines in living cells” (1), into a more straight-forward...our engineered devices, recombinase-based state machines for your own experimental needs. For more context...the paper. Why might one be interested in state machine technology? Biological research has produced a ...timing of these factors. Recombinase-based state machines (RSMs), which take on a particular DNA-sequence...terminators, etc) are composed. Fear not – the state machine design space is highly degenerate and often times...Roquet et al., Synthetic recombinase-based state machines in living cells. Science. 353, aad8559 (2016).... recombinase-based state machines...

...ab1 chromatogram file 4Peaks (Mac) SnapGene Viewer (Mac/PC) FinchTV(Mac/PC) Chromas (PC) 2. You should...

...web and desktop version that works with Windows, Mac, and Linux operating systems.  Zotero  Zotero is ...is a desktop based program that’s compatible with Mac, Windows, and Linux.  Mendeley and Zotero have a ...

...is magnetic bead-activated cell sorting, or MACS. But MACS usually only allows for sorting based on a ...achieves greater purity of your sorted cell sample than MACS. An effective strategy to sort out your cells of...

...This post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No ... cycle.  Many thanks to our Guest Blogger, Sean Mac Fhearraigh! Seán was a PhD student at University...

...al, 2023 AAV.CAP-Mac CNS: neurons (rats, infant green monkey, rhesus macaque); vasculature (adult...Old World monkeys AAV.CAP-Mac is largely neurotropic in infant rhesus macaques and green monkeys (Chuapoco...caption adapted from Campos et al., 2023. AAV.CAP-Mac is a systemic capsid, developed and deposited by ...selected from a library screened in marmosets. AAV.CAP-Mac effectively transduces vasculature in adult marmosets...astrocytes (Chuapoco et al., 2023). Additionally, AAV.CAP-Mac has a lower transduction rate than AAV9 in the liver... rhesus macaques (Chen et al., 2022). AAV.MaCPNS2 is slightly more effective than AAV.MaCPNS1 at transducing...rats); CNS neurons (infant rhesus macaques) Mice, rats, rhesus macaques No (mechanism unknown)   Chen...

...source and available for desktop use for Windows, Mac OS X, and Linux. Inkscape is versatile and has a ...

...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well...death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career ...

... Pubmed PMID: 3325793 Schumacher MA (2012) Bacterial plasmid partition machinery: a minimalist approach...origin of replication and the replication control machinery (Need a refresher on the Origin of Replication...they compete for the same replication control machinery within the cell. Most plasmids encode a negative...through inhibiting translation of replication machinery proteins, or directly inhibit replication by binding...origin of replication and blocking replication machinery (del Solar, 1998). As the copy number of the plasmid... structure that physically blocks replication machinery when plasmid copy number is high (del Solar et...replication of each plasmid using unique replication machinery. However, when plasmids have the same origin of...

...and most machines have a protocol that automatically performs the normalization step.  Machines Most general-use...different brand of qPCR machine, especially if you’re using a rapid-cycle machine such as a LightCycler,...previously-generated validation data, be aware that different machines, samples, and treatments may affect the assay....running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr chemistry is ... by bubbles in wells, variations in volume, or machine surge errors and allow for well-to-well normalization... likely need to re-optimize protocols for that machine. (Teo, et al, 2002; see paper for more information...information on optimizing for LightCycler machines.) Multiplexing It is possible to multiplex qPCR when using...

...that you come into the lab wishing there was a machine that could make your life a hundred times easier...off our hands. Good news! There is probably a machine or robot out there that does exactly what you want...lot of time. Enter the Biomek FXP! We wanted a machine that would improve our transformation protocol ...played a factor in our decision to go with this machine, but we also really liked that Beckman was flexible...apart before plating. With this fine piece of machinery we have been able to transform 96 samples in about...lab team. Enter the Liquid Handler!     This machine fits on a table top and can be easily programmed...t electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful...

...Thanks to our guest blogger Cameron MacPherson! Cameron R. MacPherson is Lead Data Scientist for the Milieu...post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software and the...such as ApE or commercial workbenches such as MacVector or Benchling. Utility functions: These functions... can also download the software for use on any machine with OpenCL enabled hardware. This OpenCL dependency...genomes), then it is worthwhile buying a dedicated machine. On the offline front, there is a Python script...Pasteur, Paris. You can follow him on Twitter @CMacPhD.          References  1. Ghorbal, Mehdi, et al....

...code of life. In 1944, Oswald Avery, Colin MacLeod, and Maclyn McCarty’s experiment debunked the myth that...to stars in space ships and the rise of killer machines that exterminate entire humanity were the two ... technologies rely on the cellular DNA repair machinery, which can lead to high frequency of indel formation... the use of molecular dynamics simulations and machine learning to develop a predictive understanding ...://doi.org/10.1038/s41586-019-1711-4 Avery OT, MacLeod CM, McCarty M (1944) STUDIES ON THE CHEMICAL NATURE...

...experiment. Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in... cell. These so-called recombinase-based state machines could potentially be used to report upon and/or...post to learn more about recombinase-based state machines and how they function. Adapting Toehold Switches... describes R-bodies, “force generating protein machines that can extend and retract over many microns ...

... CN, Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin... Arnold’s lab and Viviana Gradinaru’s lab used machine learning to design new channelrhodopsins that are...channelrhodopsin that’s successful in vivo. Using machine-learning to overcome channelrhodopsin design challenges...challenges, the Arnold and Gradinaru Labs developed a machine-learning model to help them construct “designer...

... passes through the machine’s lasers and gives off a signal read by the machine’s sensors. (Usually this...or unbound antibodies.) Each event read by the machine is plotted as a dot on an X,Y axis to generate ...variations in fluorescent signals, and how the machine reads/interprets the signals, the axis is instead...populations. This shape is called a ‘gate’; it tells the machine that everything inside the gate is one population...

...your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules of interest...process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically or chemically...soluble molecule that does not interfere with the macromolecules it is added to. Biotin also has an exposed ...developed several methods to chemically biotinylate macromolecules of interest both in vitro and in vivo. Biotinylation...

...The most commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as ...the system. Since then, multiple biotech and pharmaceutical companies (along with some research laboratories...the expression of recombinant proteins by the pharmaceutical and biotech industries as a protein expression...specifically glycosylation. The native glycosylation machinery of K. phaffii lacks certain terminal mannose glycopeptides...

...therapeutic proteins. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft...further differentiated into dentritic cells or macrophages through cytokine induction (Groell et.al, 2018...Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis: From Manufacturing to Preclinical...