...rhodopsins Mac Light-activated outward proton pump from Leptosphaeria maculans 540 eMac 3.0 Mac with addition...Leptosphaeria maculans . Mac and its variants allow for inhibition of neurons using blue-green light. Mac Variants...Archaerhodopsin plasmids . Wild-Type Mac Leptosphaeria rhodopsin (Mac) is a blue-green light-activated proton...Variants. Mac variants have been engineered to include enhancements such as: Improved photocurrent amplitude...amplitude Example: eMac3.0 Browse Leptosphaeria plasmids . Glossary of Microbial Opsin Variants Opsin Type...

...the antipsychotic clozapine and seems to be pharmacologically inert in mice and rats. However, CNO is back-metabolized...neurons through manipulation of ion channels arePharmacologically Selective Actuator Modules (PSAMs, pronounced...respond to specific small molecules termed Pharmacologically Selective Effector Molecules (PSEMs). PSAM...pre-requisite for mimicking clozapine’s actions? Psychopharmacology. 178:451–460. PMID: 15765260 (Link opens ...cAMP Production in Striatopallidal Neurons. Neuropsychopharmacol. 38:854–862. PMID: 23303063 (Link opens ...arrestin-biased designer G protein-coupled receptor. Mol Pharmacol. 82:575–82. PMID: 22821234 (Link opens in a new...

...using a sequencing machine that recognizes the different fluorescent labels. The machine detects which fluorescently...replication (by bacteria) by recruiting DNA replication machinery. The ori is critical for the ability of the plasmid... in the bacteria by bacterial transcriptional machinery. Multiple Cloning Site (MCS) Short segment of ...promoter is designed to recruit transcriptional machinery from a particular organism or group of organisms... when sequencing is not 100% efficient and the machine cannot distinguish between the 4 labelled nucleotides...

...can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to distinguish...either X-ray film in a darkroom or an image capture machine. Special considerations The primary antibody must...direction based on the emission(s) read by the machine. Controls for cell sorting methods Flow cytometry...overlap to avoid confusing data. Check that your machine has appropriate lasers and filters for each fluorophore...

...the virus depends on the cell’s DNA replication machinery to synthesize the complementary strand, transgene...

...reagents may be sufficient to express the CRISPR machinery. For more difficult cells (e.g. primary cells)...

...Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015)...