... .ab1 files, such as 4Peaks (Mac), SnapGene Viewer (Mac/PC), FinchTV (Mac/PC), Sequence Scanner (PC), ...

... - AAV.CAP-Mac For rats: Broad tropism - AAV-PHP.eB Cell type-biased: Neurons - AAV.CAP-Mac Brain vascular...rhesus macaques: Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2 Cell type-biased: Neurons - AAV.CAP-Mac, AAV9...Caltech Systemic Capsids PHP.eB PHP.S PHP.V1 MaCPNS1 MaCPNS2 CAP-B10 CAP-B22 AAV9-X1.1 As part of our Viral...Gradinaru MaCPNS1 These viral vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid...Boyden MaCPNS2 These viral vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid...Pubmed 32313222 (Link opens in a new window) MaCPNS1, MaCPNS2: Chen et al., 2022 Pubmed 35643078 (Link opens...NHPs: Broad tropism - AAV-MaCPNS2, AAV-PHP.S Sensory nervous system - AAV-MaCPNS1 Return to top...

...bias observed in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed...primary cells Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific...rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human...2023). They found the best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier...to identification and characterization of AAV.CAP-Mac. Image reproduced from Chuapoco, Flytzanis, Goeden...2023, under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco, M.R., Flytzanis, N.C.,...

... our paper, “Synthetic recombinase-based state machines in living cells” (1), into a more straight-forward...our engineered devices, recombinase-based state machines for your own experimental needs. For more context...the paper. Why might one be interested in state machine technology? Biological research has produced a ...timing of these factors. Recombinase-based state machines (RSMs), which take on a particular DNA-sequence...terminators, etc) are composed. Fear not – the state machine design space is highly degenerate and often times...Roquet et al., Synthetic recombinase-based state machines in living cells. Science. 353, aad8559 (2016).... recombinase-based state machines...

...ab1 chromatogram file 4Peaks (Mac) SnapGene Viewer (Mac/PC) FinchTV(Mac/PC) Chromas (PC) 2. You should...

...web and desktop version that works with Windows, Mac, and Linux operating systems.  Zotero  Zotero is ...is a desktop based program that’s compatible with Mac, Windows, and Linux.  Mendeley and Zotero have a ...

...is magnetic bead-activated cell sorting, or MACS. But MACS usually only allows for sorting based on a ...achieves greater purity of your sorted cell sample than MACS. An effective strategy to sort out your cells of...

...This post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No ... cycle.  Many thanks to our Guest Blogger, Sean Mac Fhearraigh! Seán was a PhD student at University...

...al, 2023 AAV.CAP-Mac CNS: neurons (rats, infant green monkey, rhesus macaque); vasculature (adult...Old World monkeys AAV.CAP-Mac is largely neurotropic in infant rhesus macaques and green monkeys (Chuapoco...caption adapted from Campos et al., 2023. AAV.CAP-Mac is a systemic capsid, developed and deposited by ...selected from a library screened in marmosets. AAV.CAP-Mac effectively transduces vasculature in adult marmosets...astrocytes (Chuapoco et al., 2023). Additionally, AAV.CAP-Mac has a lower transduction rate than AAV9 in the liver... rhesus macaques (Chen et al., 2022). AAV.MaCPNS2 is slightly more effective than AAV.MaCPNS1 at transducing...rats); CNS neurons (infant rhesus macaques) Mice, rats, rhesus macaques No (mechanism unknown)   Chen...

...source and available for desktop use for Windows, Mac OS X, and Linux. Inkscape is versatile and has a ...

...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well...death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career ...

...rhodopsins Mac Light-activated outward proton pump from Leptosphaeria maculans 540 eMac 3.0 Mac with addition...Leptosphaeria maculans . Mac and its variants allow for inhibition of neurons using blue-green light. Mac Variants...Archaerhodopsin plasmids . Wild-Type Mac Leptosphaeria rhodopsin (Mac) is a blue-green light-activated proton...Variants. Mac variants have been engineered to include enhancements such as: Improved photocurrent amplitude...amplitude Example: eMac3.0 Browse Leptosphaeria plasmids . Glossary of Microbial Opsin Variants Opsin Type...

...isolation of cells, organelles, lipoproteins, and macromolecules. Importantly, iodixanol is inert and non-toxic...NaCl to destabilize ionic interactions between macromolecules. The 40% and 25% steps are used to remove contaminants...option #1. Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua...

...2 O 0.2 μL Taq DNA Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design...added to a master mix. Place reaction tubes in PCR machine. Set annealing temperature 5°C below the primer...liquid to the bottom before placing it in the PCR machine. Diluting Primers Most people order primers from...

...receptor 3 and 4 subunit) CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7 KIR2DL1 killer cell immunoglobulin-...prosome, macropain) 26S subunit, ATPase, 3 MGC8487, TBP1 PSMC4 proteasome (prosome, macropain) 26S subunit...prosome, macropain) 26S subunit, non-ATPase, 10 dJ889N15.2, p28 PSMD11 proteasome (prosome, macropain) 26S...prosome, macropain) 26S subunit, non-ATPase, 14 PAD1, POH1, rpn11 PSMD2 proteasome (prosome, macropain) 26S...prosome, macropain) 26S subunit, non-ATPase, 3 P58, RPN3, S3 PSMD4 proteasome (prosome, macropain) 26S subunit...ligand 18 (pulmonary and activation-regulated) AMAC-1, AMAC1, CKb7, DC-CK1, DCCK1, MIP-4, PARC, SCYA18 CCL19...

...pUCmini-iCAP-AAV.MaCPNS1 MaCPNS1 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS1 Cap controlled...pUCmini-iCAP-AAV.MaCPNS2 MaCPNS2 AAV packaging plasmid (non-standard), expressing Rep2 and MaCPNS2 Cap controlled...production of PAL1A, an AAV capsid with CNS tropism in macaques. Sabeti 196684 kiCAP-AAV-PAL1B PAL1B Rep/Cap plasmid...production of PAL1B, an AAV capsid with CNS tropism in macaques. Sabeti 196685 kiCAP-AAV-PAL1C PAL1C Rep/Cap plasmid...production of PAL1C, an AAV capsid with CNS tropism in macaques. Sabeti 196686 kiCAP-AAV-PAL1D PAL1D Rep/Cap plasmid...production of PAL1D, an AAV capsid with CNS tropism in macaques. Sabeti 196687 kiCAP-AAV-M.Fas.1 M.Fas.1 Rep/Cap... of M.Fas.1, an AAV capsid with CNS tropism in macaques. Sabeti 196688 kiCAP-AAV-M.Fas.2 M.Fas.2 Rep/Cap...

...minutes at 95°C in a PCR machine or in a beaker of boiling water. If using a PCR machine, incubate the sample...

...Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10 CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter CAG CaMKIIa CMV Dlx EF1a... 2, 5, 8, 9, rg*, PHPeB, CAP-B10, CAP-B22, MaCPNS1, MaCPNS2, AAV9-X1.1 Boyden 44332 pZac2.1 gfaABC1D-tdTomato...

...translation machinery. To expand the genetic code, 4 major changes to the standard translation machinery are ...-L-lysine Bacterial TAG David Liu 104071 pTECH-MmAcK3RS(IPYE) AcK3RS M. mazei Nε-acetyl-L-lysine Bacterial...Mammalian TAG Ivana Nikić-Spiegel 182537 pEVOL-MmAcKRS1-PylTUUA PylRS M. mazei Bacterial TAA Wenshe Liu...

...can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to distinguish...either X-ray film in a darkroom or an image capture machine. Special considerations The primary antibody must...direction based on the emission(s) read by the machine. Controls for cell sorting methods Flow cytometry...overlap to avoid confusing data. Check that your machine has appropriate lasers and filters for each fluorophore...