...selection curve below. Carbenicillin is used here in place of ampicillin because carbenicillin is more stable,...concentrations of carbenicillin plated over-agar. Control Plate with No Carbenicillin Plate shows a lawn...Plating of Carbenicillin. The above graph displays the stock concentration of Carbenicillin stock used...protocol will focus specifically on selection with carbenicillin; however it can be adapted for any antibiotic...antibiotic High concentration (100 mg/mL, 1000x) carbenicillin stock solution in sterile water (or other antibiotic...coli transformed with a plasmid containing the carbenicillin (amp) resistance gene (or other antibiotic resistance... resistance) Procedure Day 1 Prepare carbenicillin to a concentration of 1 mg/mL – 4 mg/mL in LB medium...

...agar plates containing 100 μg/mL ampicillin or carbenicillin (an ampicillin analog). Due to the long terminal...Bioanalytical: #AB01200-02000 Ampicillin VWR: #7177-48-2. Use at 100 μg/mL. Carbenicillin VWR: #80030-956. Use ...from each ligation into LB + 100 μg/mL ampicillin or carbenicillin. Day 2: 2. Spin down the cultures and...and cool to 55°C. Add 1mL of 100mg/mL ampicillin or carbenicillin to obtain a final concentration of 100...tract, cPPT, improves transduction efficiency by facilitating nuclear import of the vector’s preintegration...ori f1 bacterial origin of replication. Amp R Ampicillin resistance gene for selection of pLKO.1 plasmid...#11995 Fetal Bovine Serum (FBS) Invitrogen: #16000 Penicillin/Streptomycin Invitrogen: #15140-122 Polypropylene...

...dissolved in dH 2 O. *Carbenicillin can be used in place of ampicillin. Carbenicillin is more stable, so ...Working Concentration Ampicillin 100 mg/mL 100 µg/mL Bleocin 5 mg/mL 5 µg/mL Carbenicillin* 100 mg/mL 100 µg...plates with a final concentration of 100 ug/mL ampicillin, you should make a stock solution of 100,000 .../mL (100 mg/mL). Simply measure out 100 mg of ampicillin powder, add it to 1 mL of water, dissolve by ...

...Recommended Concentration Ampicillin 100 µg/mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol...

...containing agar plate. This step is not critical for Ampicillin resistance but is much more important for other...heat-shock Shorten or skip the outgrowth (for Ampicillin resistance it is ok to completely skip the outgrowth...

...RNase-free Glycogen may be used as a carrier to facilitate RNA precipitation. This does not affect the quality...

...tooth pick at an angle, the way you would hold a pencil, so that you can make a broad stroke. Only touch...

...file) provided by your sequencing company or core facility The corresponding primer sequence used for each...

...vial of cells after 20–30 passages. Do not add penicillin/streptomycin to the media, as it can affect viability...

...vial of cells after 20–30 passages. Do not add penicillin/streptomycin to the media, as it can affect viability...

...vial of cells after 20-30 passages. Do not add penicillin/streptomycin to the media, as it can affect viability...