...genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient genome editing tool that allows ...lysogeny broth (LB) agar plate with 100 μg/ml ampicillin and incubate O/N at 37 °C. Pick 2 - 3 colonies...all steps consists of DMEM supplemented with 2% penicillin/ streptomycin and 1% L-glutamine, which is used... per well for a final volume of 150 μl. This facilitates screening by allowing a multichannel pipette ...

...performed by the facility that produced the viral vector lot. Contact us to determine which facility produced ...collaboration with viral vector manufacturing facilities, such as the University of Pennsylvania Vector...

...cholecystokinin interneurons in the basal amygdala facilitates fear extinction. eNeuro. 6(6). PubMed (Link opens...Expressing Neurons in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing...Expressing Neurons in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation...

...harnessed the type II CRISPR nuclease system to facilitate genome editing in mammalian cells (Cong et al...AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top...hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination and fluorescence assisted...

...leading synthetic biologists around the world to facilitate the sharing of parts and information. Addgene...Multiplexed/Programmable Gene Networks Mascher Lab Bacillus BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt...

...islandicus Plasmids for Bacteria Species Anabaena sp. Bacillus sp. Burkholderia sp. Bordetella pertussis Campylobacter...sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. Haemophilus influenzae Helicobacter pylori...

...pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-quality quality...all-inclusive of several important steps: MTA facilitation for the transfer plasmid, including legal approval...

...marker for all plasmids in this collection is ampicillin. Find more information on the enzymes and protocols...BGT beta-glucosyltransferase 165531 pOpen-UDG UDG Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine...

...cell protein 4) is involved in beating of airway cilia.( 7 ) Cytokines - SARS-CoV-2 infection induces a... - Kogiso H, et al, 2020, Int J Mol Sci Airway Ciliary Beating Affected by the Pcp4 Dose-Dependent [Ca...

... Voeltz 89472 GFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 GFP Ken-Ichi Takemaru 89770 pLenti-EGFP-hChibby1...pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 EGFP Ken-Ichi Takemaru 118084 pLenti-EB1-EGFP...

...Recombinant mAb Plasmid Collection . The NeuroMab Facility and James Trimmer have produced monoclonal antibodies...Service . Addgene provides a viral service to help facilitate scientific research. Addgene's AAV are made from...

...The NeuroMab Facility and James Trimmer have produced monoclonal antibodies validated for neuroscience...Protocols References The UC Davis/NIH NeuroMab Facility (Link opens in a new window) together with the...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary...collection . Protocols The UC Davis/NIH NeuroMab Facility has several protocols available that may be helpful...

...higher levels in mice than P1 bacteriophage Cre, facilitating high rates of Cre-driven recombination. CREM... knocked in to a mouse at the Rosa26 locus to facilitate Cas9 mediated genome editing in multiple tissues...

...Lin Tian Serotonin Cilia-targeted serotonin sensor GRAB-HTR6 A serotonergic axon-cilium synapse drives nuclear...

...8 CKLFSF8, CKLFSF8-V2 CNTF ciliary neurotrophic factor HCNTF CNTFR ciliary neurotrophic factor receptor... IL1RRP2 IL1RN interleukin 1 receptor antagonist ICIL-1RA, IL-1ra3, IL1F3, IL1RA, IRAP, MGC10430 IL2 interleukin...IL1RRP2, IL36R IL1RN interleukin 1 receptor antagonist ICIL-1RA, IL-1ra3, IL1F3, IL1RA, IRAP, MGC10430 IL2 interleukin...

...distributes ready-to-use recombinant antibodies to help facilitate scientific research. We are building a collection...

...identification of potential RGN target sites and to facilitate design of short oligonucleotides needed to construct...

...BSL-3) agent, SARS-CoV-2 requires specialized facilities for the study of the live virus. Alternatively...

...shared experience for the research community and facilitates scientific collaborations between institutions...

...these approaches have created this resource to facilitate open and free exchange of these expression plasmids...