...under from the CB promoter pCDH-CB-iCre 72257 Express iCre under from the CB promoter pCDH-CB-FLPe-P2A-...the CB promoter pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1...gene from the CMV promoter pCDH-CMV 72265 Express gene of interest from the CMV promoter pCDH-EF1 72266 ...interest from the EF-1 promoter pCDH-CB 72267 Express gene of interest from the CB promoter pCDH-PGK 72268 xpress...phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express gene of interest from the CMV promoter. WPRE has been ...from the CB promoter pCDH-EF1s 72484 Express gene of interest from a truncated EF-1 promoter pCDH-EF1-Luc2... EF1 promoter pCDH-EF1-Luc2-P2A-tdTomato 72486 Expresses Luc2 and tdTomato from the EF1 promoter pLL3.7...

...retroVirus LTR promoter. The second plasmid ( pgRNA_humanized ) contains a murine U6 promoter controlled ...vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter 46916 pU6-sgGAL4-4 Human pSico-based...vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control) 46918 pU6...contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter 46923 pSNR52-sgTET Yeast CEN...controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoter...pHR-SFFV-dCas9-BFP Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP 46911...BFP-KRAB Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB...

...inducible promoters capable of controlling eukaryotic gene expression. Several endogenous promoters had been...Tetracycline-dependent promoters are developed by placing a TRE upstream of a minimal promoter. Tetracycline Off....CMV.tTA Adenoviral; CMV promoter; see article for neuronal-specific promoter and enhancer options tTA...Collections Mouse Brain Atlas of Tet-responsive Promoters Background Tetracycline Off Tetracycline On Experimental... tetracycline, expression from a tet-inducible promoter is reduced. To use tetracycline as a regulator...these tetO sequences and the activation domain promotes expression. In the presence of tetracycline, tetracycline...are: Plasmid containing a tetracycline-dependent promoter upstream of your gene of interest tTA expression...

...pLVCT-tTR-KRAB 2nd generation; Transgene (CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick...2SM2 2nd generation; Transgene (CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick...generation; Transgene (hEF-1alpha promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick... 2nd generation; Transgene (hPGK promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick... 2nd generation; Transgene (hPGK promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick...generation; Transgene (hUbiquitin promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick...2nd generation; Transgene (hPrion promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick...

...-mCherry Expression of hM4D(Gi) using the CD68 promoter. Bryan Roth 75033-AAV9 pAAV CD68-hM4D(Gi)-mCherry...mCherry Expression of hM4D(Gi) using the CD68 promoter. Bryan Roth 83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell...An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed...Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed...recombinase-dependent expression of mNeonGreen from the CAG promoter Viviana Gradinaru 100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA...expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru 104055-AAV2 pAAV-CAG-eYFP An...expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104055-AAV5 pAAV-CAG-eYFP An...

...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication...Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable ...Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable ...Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter Selectable...PI Publication Parasites ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...

...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication...Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI...Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...

...expression of gRNA under control of the Drosophila U6:1 promoter Bullock 49409 pCFD2-dU6:2gRNA expression of gRNA...gRNA under control of the Drosophila U6:2 promoter Bullock 49410 pCFD3-dU6:3gRNA expression of gRNA under...under control of the Drosophila U6:3 promoter Bullock 49411 pCFD4-U6:1_U6:3tandemgRNAs expression of two...pAct-Cas9 Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering...pnos-Cas9-nos Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering...dCas9 Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity...nos Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity...

... from the eft-3 promoter, while pMB66 and pMB67 use the hsp-16.48 heat shock promoter. All vectors include....1) promoter. 47945 pMB63 : Expresses C. elegans optimized Cas9 from the eft-3 (eef-1A.1) promoter. 47946...-16.48 promoter. 47947 pMB67 : Expresses C. elegans optimized Cas9 from the hap-16.48 promoter. Please... allows in vitro production of RNA from the T7 promoter, while pMB70 is designed for in vivo transcrition... generate synthetic guide RNA in vitro from T7 promotor. 47943 pMB70 : Can be used to express a CRISPR...

...lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR...for directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas...-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 ...edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence...

...lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function... on the opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants...-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused...edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9...Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence...

...plasmid with a promoter that will be functional in your host organism. Host Relevant Promoters Representative...vector (PGK1 promoter, 2μ) pXP316 - Yeast expression vector (TEF1 promoter, CEN/ARS) pAG304GAL-ccdB...vector (PGK1 promoter, CEN/ARS) pXP222 - Yeast expression vector (PGK1 promoter, 2μ) pAG305GAL-ccdB...Representative Empty Backbones Promoter Measure promoter strength pBV-Luc - Luciferase ... vectors with various markers, promoters, etc pRS420 - Yeast expression ... expression under the metallothionein promoter pACU2 - Modified pUAST vector containing... transgenes Worm unc-54, variety of worm gene promoters C. elegans vector kit - Collection...

...of 5' promoter/enhancer regions Joshua Mendell 60323 pGL4.23-GW Firefly Insertion of 5' promoter/enhancer...investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect...available that are driven by a strong constitutive promoter and can be used to monitor transfection or viral...plasmids by using the bacterial ORI as the core promoter, which was a source of false positives with the...Description PI 16539 pBV-Luc Firefly Insertion of 5' promoter/enhancer regions Bert Vogelstein 12178 pIS0 Firefly... Renilla luciferase under the control of a CMV promoter is present for normalization William Kaelin 71248...71248 pXPG Firefly Insertion of 5' promoter/enhancer regions. Mutations within the luciferase gene allow...

...lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base Edit Catalytically...opposite strand) mutations. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas...repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically...Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence...to your specific locus. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Purify A catalytically...

... the H1 promoter. 53187 pmU6-gRNA : Expresses the S. pyogenes sgRNA from the mouse U6 promoter. 53188 ... human U6 promoter. 53189 ph7SK-gRNA : Expresses the S. pyogenes sgRNA from the 7SK promoter. 53190 pLV...activator VP64. When directed to its target in gene promoters by the sgRNA expressed in trans from pSPgRNA, ...downstream genes. Multiple sgRNAs targeted to the same promoter act synergistically to activate gene expression...

...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication...Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable ...Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence...

...Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable ...Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions...

...containing promoters that drive general and species-specific gene expression (see the column ‘promoter’ in the...for, such as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI..., such as the promoter or fluorescent protein of choice. ID Plasmid Description Promoter PI Return to ... nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots - Vladimir... (Link opens in a new window) - The initiative promotes interdisciplinary exchange, open technologies,...

...of viruses containing transgene under a chosen promoter Vogelstein 16403 pShuttle-CMV Shuttle For production...production of viruses containing transgene under CMV promoter Vogelstein 16404 pAdTrack Shuttle For production...GFP-trackable viruses containing transgene under a chosen promoter Vogelstein 16405 pAdTrack-CMV Shuttle For production...GFP-trackable viruses containing transgene under CMV promoter Vogelstein 16407 pAdEasy 2-GFP beta-gal Shuttle... transgene under Neuron-specific enolase (NSE) promoter Bamburg 50959 ShuttleMCP Shuttle For production...viruses containing transgene under mouse cofilin promoter (MCP) Bamburg 62621 pShuttle-CMV-F2A-T2A-Venus...

...expression. pCW-LIC 26098 3x Tac promoter (hybrid of trp and lac promoters with higher expression upon induction...function, structure, and interactions! Plasmid ID Promoter Epitope Tag(s) ID Purpose pDEST-HisMBP 11085 Tac... Various Plasmids pTrc, hybrid of trp and lac promoters (Lactose/IPTG inducible) Various combinations ...with your gene/protein of interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids... from Promega. Contain a variety of inducible promoters, their associated transcription factors, and reporter...by the quorum sensing molecule, 3OC6HSL, from a promoter repressed by EsaR. Designed to be used with the... sensitivities to 3OC6HSL. The EsaR-responsive promoter can be found in pCS-PesaRlux controlling luciferase...