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Showing: 1 - 9 of 9 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ...) Pro-Tip Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...viscosity of PEG. Stirring under medium heat will promote faster dissolution. Adjust the pH to ~7.4. Autoclave...

  2. Gibson Assembly Protocol

    Type
    Protocol
    ...technique is especially useful for introducing promoters, terminators, and other short sequences into the...

  3. Fluorescence Titering Assay

    Type
    Protocol
    ... Pro-Tips Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...

  4. General Transfection

    Type
    Protocol
    .... Pro-Tip Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...

  5. Lentivirus Production

    Type
    Protocol
    .... Pro-Tip Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...

  6. Colony Formation Titering Assay

    Type
    Protocol
    .... Pro-Tip Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...10878/ . Description Vector Element U6 Human U6 promoter drives RNA Polymerase III transcription for generation...transduced cells. hPGK Human phosphoglycerate kinase promoter drives expression of puromycin. Puro R Puromycin...
Showing: 1 - 9 of 9 results