... Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection...Plasmids ID Plasmid Gene/Insert Pooled Library The Multiplexed Overexpression of Regulatory Factors (MORF) pooled...

...Which FP Should I Use? Blog: How to Choose a FP For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent...

...4-COMP-his-stop Type II expression CD69-His-bio Multi TM expression CD36-bio-His Plasmid collections exist...proteins are expressed as both monomeric baits and multimerised enzyme-tagged preys. Ectodomain inserts: The...

...Freemont Lab MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae - Dueber Lab Yeast...Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part fusion proteins... in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression in E. coli - Koffas...

...vectors containing several cassettes and generate multi-construct transgenic plants - Jan Lohmann. MoClo... seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants... of useful plant CRISPR resources include: A Multipurpose Toolkit enables targeted, specific modification...CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants - Yao-Guang...expression vectors: A modular plasmid assembly kit for multigene expression, gene silencing, and silencing rescue...Infrastructure Kit for hierarchical assembly of plant multigene constructs - Johannes Stuttmann. MoChlo: Modular...

...following in 2015. Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain effector) Type I ...not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed in the...genome engineering, offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR...the tool’s specificity, orthogonality, and multiplexibility in various species, as well as the development...being able to target every locus in every genome. Multiple Cas9 orthologs have also been characterized. Staphylococcus...distinct PAM sites that do not cross react, allowing multiple Cas9s to be used together. Researchers have engineered...Cpf1's small size also makes it suitable for multiplexing and use in AAV. Scientists have also developed...

...Synthetically Phosphorylated Isomers (iSPI) Described in: A multi-purpose, regenerable, proteome-scale, human phosphoserine...orthogonal translation system to make singly or multiply phosphorylated proteins (Pirman et al., Nat Comm...

...vectors with various resistances. Comprehensive multi-cloning site compatible with standard cloning using...use the same restriction enzymes for cloning so multiple constructs can be made and tested at once. Return...

...luciferase in plants Diego Orzaez 118069 MLRV Multiple Multiple Multi-luciferase reporter vector including transcriptional...Nano-lanterns) to allow for BRET-based in vivo imaging of multiple cellular processes, biosensors, or optogenetic...

...Which FP Should I Use? Blog: How to Choose a FP For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent...proteins Insect Sutherland Lab Plasmids - For multicistronic expression of GFP and/or mCherry, with neomycin...

...five human isotypes. Some isotypes naturally form multi-antibody units, while others exist as monomers. ...antibody in assays with multiple targets, and is described in more detail in the Multiplex Assays section below...differentiation between multiple antibodies. They are often used in assays that have multiple proteins to detect... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...allows for the amplification of signals by having multiple secondary antibodies binding to a primary antibody..., which is typically the number of antibodies multiplied by two, as each antibody has two binding sites...

... Many different activators exist, including the multi-plasmid SAM system. Select Expression System To ...more about Cas9 multiplexing and Cas12a (Cpf1) multiplexing . Browse Plasmids: Multiplex gRNA vectors Genome-Wide...two gRNA target sites Modifying multiple genes at once Current multiplex CRISPR systems enable researchers...genetic loci by cloning multiple gRNAs into a single plasmid. These multiplex gRNA vectors can conceivably...in living cells. Multicolor CRISPR imaging allows for simultaneous tracking of multiple genomic loci in...Base Edit Activation or Repression Epigenetics Multiplex Genome Engineering Genome-wide Screening What ... Both types of base editors are available with multiple Cas9 variants including high fidelity Cas9’s. ...

...aureus Joung Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors...vectors. These systems can be used to express multiple gRNAs from a single plasmid with or without expression...expression of Cas9. Highlighted below are some multiplex gRNA vectors from our collection. Plasmids Expression...Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit Mammalian A system for constructing...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease... available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering from a single...Gate assembly. Gersbach MuLE System Mammalian MultiSite Gateway recombination-based cloning of complex...

...targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...or mitochondria Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...Campbell Voltage Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters...sensors for simultaneous monitoring of Zn(2)(+) in multiple cellular locations. PLoS One. 2012;7(11):e49371...Biol. 2010 Apr 16;5(4):415-25. Martin Webb ATP Multicolor palette of ATP indicators (MaLion) RGB-color ...

... Multisystem proteinopathy, ALS Nicolas Fawzi 98667 hnRNPA2_LC_WT_S329C HNRNPA2B1 His T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy... T7 Multisystem proteinopathy, ALS Nicolas Fawzi 157865 hnRNPA2_RRM1 HNRNPA2B1 His T7 Multisystem proteinopathy...Nicolas Fawzi 98657 hnRNPA2_LC_WT HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98658 hnRNPA2...hnRNPA2_LC_D290V HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98659 hnRNPA2_LC_D220V HNRNPA2B1...

...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to...molecular payloads in target populations defined by multiple parameters....Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to...molecular payloads in target populations defined by multiple parameters. Examples of intersectional cell population...definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations.... This approach has been broadly applied using multiple recombinase-expression ssitrategies to define ...2014. Targeting cells with single vectors using multiple-feature Boolean logic. Nat Methods 11(7):763-72...

... markers, respectively, or constructs in which multiple sgRNAs may be expressed from a single plasmid....non-deletion and deletion bands ( Figure 2 ). Consider multiplexing the “deletion” and “non-deletion” PCR primer...primer pairs in a single reaction. Optimize multiplexing in a polyclonal population ( i.e., bulk sorted cells...amplicons be easily resolved on an agarose gel for multiplexing. Screening CRISPR/Cas9 Clones for Deletions ...μl. This facilitates screening by allowing a multichannel pipette to be used for the remainder of the ...in each well) to a 96-well PCR plate using a multichannel pipette. Centrifuge PCR plate at 400 x g for...agar plates with the relevant antibiotic. Select multiple colonies, mini-prep each one, and subject each...

...for different genomic loci, capable of detecting multiple genomic loci, and compatible with live cell imaging...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive...genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting...

... activates the expression of downstream genes. Multiple sgRNAs targeted to the same promoter act synergistically... collection of reagents for CRISPR/Cas9-based multiplex genome engineering in diverse cell types. This...This set of plasmids is described in: Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral...

...body. Multipotent stem cells, such as adult (or somatic) stem cells, can differentiate into multiple cell... Stem cells are classified as pluripotent or multipotent, based on their ability to generate different...related cell lineages. Pluripotent embryonic and multipotent adult stem cells have been studied for over 30...