...pipette 1–10 µL multichannel pipette 2–50 µL multichannel pipette 20–200 µL multichannel pipette Vortex...Use multichannel pipettes for the dilution series. For dilutions 1–5, use the 1–10 µL multichannel pipette...polystyrene reagent reservoir. Using a 20–200 µL multichannel pipette, carefully add the dilution buffer to...to 5 µL. For dilutions 6–8, use the 20–200 µL multichannel pipette set to 50 µL. For mixing between dilutions...dilutions, use the 20–200 µL multichannel pipette set to 90 µL and mix by pipetting the liquid up and down...cartridge into the cartridge holder. Using a 2–50 µL multichannel pipet, load 20 µL of the reaction mixtures into...polystyrene reagent reservoir. Using the 20–200 µL multichannel pipet, load 70 µL of droplet generation oil ...

...pipette 1–10 µL multichannel pipette 2–50 µL multichannel pipette 20–200 µL multichannel pipette Reagents...i.e master mix + water Whenever possible use a multichannel pipet to minimize pipetting error and variability... samples very well by pipetting back and forth multiple times at each step Reagent Preparation Master ...immediately before use. Use a reservoir and a multichannel pipette to dispense the master mix into the ...time to make a new one. When developing the assay multiple plasmids containing ITR were tested. Plasmid #59462... results. Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs have...with >50 uL if your well contains 100 uL) Use a multichannel pipette to load the standards and samples onto...

...single channel pipette 2–50 µL multichannel pipette 20–200 µL multichannel pipette Ice bucket 96-well freezer...cartridge into the cartridge holder. Using a 2–50 µL multichannel pipet, load 20 µL of the reaction mixtures into...polystyrene reagent reservoir. Using the 20–200 µL multichannel pipet, load 70 µL of droplet generation oil ...droplets have been generated, use a 20–200 µL multichannel pipette to collect 40 µL of droplets. Pro-Tip... RPP30 in diploid cells, thus the reason for multiplying by 2. $$V = 2*{copies\ RRE \over copies\ RPP30...

... lentiviral vector allows you to control the multiplicity of infection (MOI) in downstream studies. This...recommended to subject lentiviral supernatants to multiple freeze-thaw cycles. Procedure Seed 75,000 cells...percentage exceeds 40% you risk counting cells with multiple integration events leading to underestimation ...For a more accurate titer, take the average of multiple dilutions. Sample Data Figure 1: 293T cells were...

...lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream studies. This...recommended that lentiviral supernatants be subjected to multiple freeze-thaw cycles. This protocol outlines the...provide more accurate titers as the likelihood of multiple integration events decreases as the dilution increases...For a more accurate titer, take the average of multiple dilutions. Sample Data Figure 1: A549 cells were...

...DNA molecule (single gene perhaps) to be copied multiple times by Taq Polymerase. From a single copy of...Sterile dH 2 O (variable) Note: If you are doing multiple PCRs, save time by creating a “master mix,” which...concentration of PCR product. Master Mix Preparation Multiply the volume of each reagent by the number of individual...different reactions (7 unique primer pairs), so we multiply each volume by 7. In a single 1.5mL tube combine...

...RNA degradation and avoid multiple freeze-thaw cycles. Pro-Tip To avoid multiple freeze-thaw cycles of your...

...advisable to set up multiple reactions by holding the vector concentration fixed across multiple concentrations...from their recognition sequence. This can create multiple distinct overhangs with a single enzyme, and remove...

...developed a novel method for the easy assembly of multiple linear DNA fragments (Gibson et al. , 2009). Regardless...Regardless of fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single.... Number of fragments assembled simultaneously Multiple fragments can be assembled in one reaction. However...

...consider using a multichannel pipette. Please watch Addgene’s protocol on multichannel pipetting to learn...pipette ready, push down the plunger. There will be multiple “stops” on the plunger, where you feel resistance...

...genes. TIP: Addgene recommends that you select multiple target sequences for each gene. Some sequences...aliquot the media into smaller tubes to prevent multiple freeze/thaw cycles. Back to Top F. Infecting Target... on the size of your target plate. TIP: MOI (multiplicity of infection) refers to the number of infecting... SA et. al. RNA . 2003. 9(4):493-501. PubMed . Multiply attenuated lentiviral vector achieves efficient...

...stretch of DNA to a plasmid, such as: Changing a Multiple Cloning Site (MCS) Adding short tags - ex: HA ... competent bacteria and plate. Be sure to pick multiple colonies for mini-prepping and verify insert by...

...when digested with restriction enzymes common to multiple cloning sites, will result in similar sized bands...enzyme or enzymes that will cut your plasmid into multiple fragments, you can get a very unique pattern that...

...Technique Learn how to pipette multiple samples at once using a multichannel pipette and avoid inconsistent...

...microscope. You may be wondering why there are multiple objective lenses. Each objective performs essentially...determine the final magnification of your image, multiply the magnification of your objective lens by the...

...location in your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere ...Annealed-oligo Cloning . If you are lucky enough to have multiple options for enzymes that flank your insert and...

...Chloride Close the bottle and mix by inverting multiple times Adjust pH to ~8.5 Filter sterilize through...DMEM Complete. Pipet back and forth vigorously multiple times to obtain a single cell suspension (no clumps...

... you can see that just after base 70 there are multiple peaks in the same location. Looking at the trace...

...difficulty finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut your insert...

...transforming with very small amounts of DNA or if you're multiple plasmids at once. To save money, many labs also...