...Looking For? Please let us know what you would like to see available in viral format. Please note this does...

...Looking For? Please let us know what you would like to see available in viral format. Please note this does...

...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...

...pSB700 64046 Mammalian/Lentiviral none S. pyogenes Cerulean Church LRG (Lenti_sgRNA_EFS_GFP) 65656 Mammalian...multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate...plasmid for the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid...

...Learn more about synthetic biology resources available at Addgene. Browse featured collections or depositing...systems for novel functions. Addgene is working with leading synthetic biologists around the world to facilitate...

...Addgene as well as by our trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody ...popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their applications in ...

...gene name for a full list of plasmids available. Please note that some genes may not yet have plasmids ...

...of host cellular machineries, requiring only a nuclease-deficient Cas9 (dCas9) protein and a customized.... , Jiang et al. , Hwang et al. ), is that the nuclease-deficient mutant could silence transcription on...

...rapid induction with efficient tetracycline uptake. Please search our full site for all tetracycline plasmids...Puromycin was replaced with Blasticidin selection. Please NOTE, this vector contains an insert (MAT2A) which...

...Cas to generate conditional alleles of essential nuclear and cytoplasmic proteins, which can then be depleted... This system uses a 10-day protocol, generates “clean” homozygous mutants with no co-integrated markers...

...cells with single vectors using multiple-feature Boolean logic. Nat Methods 11(7):763-72. PMID:24908100 ...Paninski L, Li B. 2017. The central amygdala controls learning in the lateral amygdala. Nat Neurosci. 20(12):...

...AGCACTGTTGCCGGCCGAGG 79006 cut S. pyogenes 26944679 Shaw Please refer to the primary article to confirm details...add validated gRNA sequences to our datatable, please download the following spreadsheet, fill out as...

...promoter for heavy chain expression, and an ER signal/leader sequence (L) for translocation of the heavy chain...promoter for light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain...Addgene as well as by our trusted partner labs. Learn More scFv Plasmid Collection The following plasmids...

...plasmids you would like to see added to this list, please email [email protected] . Synthetic Biology Plasmids...

...Addgene as well as by our trusted partner labs. To learn more about antibodies and their use, visit our Antibody...

...Addgene as well as by our trusted partner labs. To learn more about antibodies and their use, visit our Antibody...

...recently described the use CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology to modify three different sites...

...recently described the use CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology to modify four different endogenous...

...receptor bait and prey plasmids as a positive control. Please see the following references for more information...

...catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a double-strand...