...antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene ...antibodies targeting families of cell surface and secreted proteins (glypicans and integrins) and common ...common epitope tags, as well as antibody plasmids to create or express plasmid-based antibodies. The majority... Plasmids The following plasmids can be used to create or express plasmid-based antibodies. ID Plasmid...

...activity. The variety in sequence decreases the gRNA activity and increases off-target effect. Which species...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...requirements, experimental considerations, and more, created and maintained by the Doudna Lab . gRNA Plasmids...

...expressing secreted recombinant proteins from human platelets that was created for systematic screening of extracellular...receptor proteins for use with AVEXIS interaction screening Special...

...next generation architecture has been shown to increase mutation induction when using TALENs. The FokI...Gersbach These new destination vectors can be used to create TALE-transcription factors and are used at the ...TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence...these plasmids along with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation...

...list of EXPRESs plasmids used with AVEXIS for screening in zebrafish....collection of plasmids that was used for systematic screening of extracellular protein interactions to identify... references for more information: Large-scale screening for novel low-affinity extracellular protein interactions...

...antibodies to zebrafish cell surface receptors and secreted proteins....antibodies to zebrafish cell surface receptors and secreted proteins. The plasmids encode both the light and...transfecting mammalian cell lines and collecting the secreted monoclonal antibody from the supernatant. Please...

... a collection of plasmids that was created for systematic screening of extracellular protein interactions...Plasmodium vivax merozoite protein plasmids for screening protein interactions Special...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

...gene expression. Screen CRISPR libraries are a powerful tool for genome-wide screening. RNA Targeting gRNAs... a specific DNA site Transcriptional Regulation Screen Activate dCas9 fused to an activator peptide activates...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and... Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or search the...

... a collection of plasmids that was created for systematic screening of extracellular protein interactions...list of EXPRESs plasmids used with AVEXIS for screening in Plasmodium falciparum. Plasmid...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

.... How to Order Creating an Account: Before placing your order, you will need to create an account and ... our website. Placing an Order: After you have created an account, you can place orders through our website...

... Tackling Cancers’ Drug Resistance with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene...mTORC1 promotes protein and lipid synthesis, and increases energy production through glycolysis to fuel these... Tackling Cancers’ Drug Resistance with a New Screening Kit (from David Sabatini & Kris Wood) References...

...Cloning The following collections are designed for creating plant expression vectors: A modular plasmid assembly... - Martin Parniske. GreenGate Cloning System to create plant expression vectors containing several cassettes... to understand how scientific publications are created. Papers from the plant biology literature are discussed...

...is primed by the serine protease TMPRSS2. Image credit: Maya Kostman for the Innovative Genomics Institute...metalloprotease that cleaves hACE2, potentially increasing viral infectivity.( 6 ) PCP4 - (Purkinje cell... Affected by the Pcp4 Dose-Dependent [Ca 2+] i Increase in Down Syndrome Mice, Ts1Rhr . Do you have suggestions...

...When attempting to use the CRISPR/Cas9 system to create specific mutations or insertions by Homologous ..., how can cells with that mutation be selected/screened? Before starting your experiment, consider co-...sequencing, for example) on each colony in order to screen for positive ones. - Or - If your HR template has...

...C-terminal fluorescent protein tags that can be used to create fluorescent protein fusions with your protein of...heterodimer formation with dominant negative AraC decreases GFP expression. Zinc Finger Consortium: Modular...strain for performing a bacterial two-hybrid (B2H) screen of modularly assembled zinc fingers. Return to ...

...expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...community. Michael J. Fox Foundation Plasmids MJFF has created a number of plasmids for expression of variants...