...-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-CRE-M (...recombinant Cre Bacterial Rajewsky 13775 pCAG-Cre Cre-Myc CAG Mammalian Cepko 13776 pCAG-Cre:GFP Cre-GFP fusion...pOG231 NLS-Cre CMV Mammalian Wahl 19131 pIC-Cre Cre Bacterial Rajewsky 22776 MSCV CreERT2 puro Cre-ERT2 - ...mCherry-P2A-Cre Cre CMV RABV Jessell 73474 RabV CVS-N2c(deltaG)-Cre Cre CMV RABV Jessell 73717 pMLS328 Cre eft...comparison of Cre+ and Cre- cells. While Cre-lox recombination is a very useful tool, Cre expression can...Mammalian Green 11543 pPGK-Cre-bpA Cre PGK Mammalian Rajewsky 11916 pBS185 CMV-Cre Cre hCMV Mammalian Sauer ...Stainier 24593 AAV-pgk-Cre codon optimized Cre PGK AAV Aebischer 24704 GFAP-Cre Cre GFAP Mammalian Sofroniew...

...of populations expressing only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded...AND NOT Cre 137132 pAAV-Ef1a-Con/Fon-mCherry Cre AND Flp 137133 pAAV-Ef1a-Con/Foff 2.0-mCherry Cre AND NOT...AND NOT Cre 137122 pAAV-Ef1a-Con/Fon-GCaMP6F Cre AND Flp 137123 pAAV-Ef1a-Con/Foff 2.0-GCaMP6F Cre AND NOT.../VCon-EYFP Cre AND Flp AND Vcre 137165 pAAV-Ef1a-Con/Fon/VCon-GCaMP6M Cre AND Flp AND Vcre References ...expressing Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp...and Mutations 55636 pAAV-EF1a-Cre None 55632 pAAV-Ef1a-mCherry-IRES-Cre None 55637 pAAV-EF1a-Flpo None...55650 pAAV-hSyn Con/Fon EYFP Cre AND Flp 55651 pAAV-hSyn Con/Foff EYFP Cre AND NOT Flp F3/F5 137162 pAAV-Ef1a-Con...

... N/A pEMS1312 cre N/A pEMS1301 cre/EGFP/NLS N/A pEMS1308 EGFP/cre N/A pEMS1302 EGFP/cre/NLS N/A pEMS1306...promoter pEMS1293 cre CAG promoter pEMS1164 EGFP/cre CAG promoter pEMS1114 EGFP/cre/NLS CAG promoter pEMS1157... DBH pEMS1112 EGFP/cre/NLS Ple50 DBH pEMS1361 EGFP/NLS Ple51 DBH pEMS1113 EGFP/cre/NLS Ple51 DBH pEMS1362... FEV pEMS1129 EGFP/cre/NLS Ple64 FEV pEMS1398 EGFP/NLS Ple66 FEV pEMS1131 EGFP/cre/NLS Ple66 FEV pEMS1400...HAP1 pEMS1083 EGFP/cre/NLS Ple103 HAP1 pEMS1367 EGFP/NLS Ple104 HAP1 pEMS1084 EGFP/cre/NLS Ple104 HAP1 pEMS1368...Ple111 HCRT pEMS1051 cre/EGFP/NLS Ple111 HCRT pEMS1417 EGFP/NLS Ple112 HCRT pEMS1052 cre/EGFP/NLS Ple112 HCRT... HTR1A pEMS1053 cre/EGFP/NLS Ple117 HTR1A pEMS1386 EGFP/NLS Ple118 HTR1A pEMS1054 cre/EGFP/NLS Ple118 ...

...AAV-pgk-Cre PGK Cre expression Recombinases Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate...Cre.SV40 CamKII Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression with... by using this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard...-EGFP Syn EGFP, Cre-dependent Control Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent...pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control...Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Harvey 137164...expression Recombinases Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Deisseroth 55637 pAAV-EF1a-Flpo...

...Blue-wavelength fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive Serotype...CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato CAG ArchT tdTomato Cre dependent 5...eNpHR EYFP Cre dependent 9 Deisseroth 26966 AAV-Ef1a-DIO eNpHR 3.0-EYFP EF1a eNpHR 3.0 EYFP Cre dependent...soma-targeted) mRuby3 Cre dependent 9 Adesnik 137155 pAAV-nEF-Con/Fon-iC++-EYFP nEF iC++ EYFP Cre and Flp dependent...eOPN3 mScarlet Cre dependent 1, 5 Yizhar 139504 pAAV-CAG-DIO-PPO-Venus CAG PPO Venus Cre dependent 5 Bruchas...137142 pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2 mCherry Cre and Flp dependent 8 Deisseroth 137143 pAAV-nEF-Con...pAAV-nEF-Con/Foff 2.0-ChR2-mCherry nEF ChR2 mCherry Cre dependent 8 Deisseroth 137144 pAAV-nEF-Coff/Fon-ChR2-mCherry...

...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive...none Cre dependent 1, 5, 9, rg* GENIE 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m none Cre-dependent...none Cre dependent 1, rg*, PHPeB Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG jGCaMP7s none Cre dependent... none Cre dependent 1, 9 Kim , GENIE 100839 pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 CAG GCaMP6m none Cre dependent...none Cre and Flp dependent 8 Deisseroth 137120 pAAV-Ef1a-Con/Foff 2.0-GCaMP6M EF1a GCaMP6m none Cre dependent...

... Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive (non-cre-dependent) Clear...None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity Cre-dependent...EF1a EYFP Cre dependent 1, 2, 5, 9, rg* Deisseroth 28306 pAAV-FLEX-tdTomato CAG tdTomato Cre dependent...nls-EGFP Cre dependent 1, 2, 5, 8, 9, rg* Harvey 131002 pAAV-Ef1a-DIO mScarlet EF1a mScarlet Cre dependent... hSyn EGFP Cre dependent 1, 2, 5, 8, rg* Roth 50459 pAAV-hSyn-DIO-mCherry hSyn mCherry Cre dependent 1...pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent 1, 2, 5, 8, 9, rg* Zeng 59331 pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent 1 Wickersham...pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1 Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre dependent 1,...

...99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent Cre-dependent expression of cytoplasmic tdTomato...FLEx mGFP-2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP...Serotype PI 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent expression of diphtheria toxin receptor...45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer...Serotype PI 98747 pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent EGFP-tagged ribosomal L10a 5, rg* Heintz...

...system can be implemented by delivering Cre and sgRNA to a Cre-dependent mouse or sgRNA to a constitutively...plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors...targeted to NeuN, plus Cre recombinase 60228 : sgRNA targeted to LacZ, plus Cre recombinase 60229 : sgRNA... cloning backbone with Cre recombinase 60230 : sgRNA targeted to NeuN, plus Cre recombinase-2A-EGFP-KASH...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase...sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR This plasmid contains two expression cassettes, Cre recombinase and...

...dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent) Serotype...Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D(...Gq)-mCherry hM3D(Gq) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 44362 pAAV-hSyn-DIO-hM4D...Gi)-mCherry hM4D(Gi) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Roth 50454 pAAV-hSyn-DIO-HA-hM3D(Gq)-...hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Roth 50455 pAAV-hSyn-DIO-HA-hM4D(Gi)-...hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Roth 50458 pAAV-hSyn-DIO-rM3D(Gs)-mCherry...

...in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors...PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic...Roth 83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain...Fishell 83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain...Fishell 83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain...Fishell 83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain...Fishell 83894-AAVrg pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain...

...Deisseroth 55636 pAAV-EF1a-Cre EF1a none rg* Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9, rg...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1,....eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson EF1a Promoter 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a ...105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB Wilson GfaABC1D Promoter 196410 AAV-GfaABC1D-Cre-4x6T ...Promoter 107788 AAV.rTH.PI.Cre.SV40 rTH none 9, rg* Wilson PGK Promoter 24593 AAV-pgk-Cre PGK none rg* Aebischer...51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8...8, rg* Deisseroth Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI CaMKII Promoter 105551 pENN....

...Cre-expression Cre Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression...Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent ...sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent...sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG Calcium sensor, Cre-dependent...Activator, Cre-dependent ChR2 Deisseroth 127090 pAAV-CAG-DIO-ChR2(H134R)-eYFP CAG Activator, Cre-dependent... Controls 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden 37825 pAAV-CAG-GFP CAG GFP...

...system. Didier Trono Cre-regulated Lentiviral shRNA Lentiviral vectors for conditional (Cre-lox) shRNA expression...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...Mammalian shRNA Tools for RNAi Constitutive Lentiviral Cre-regulated Lentiviral Tet-regulated Lentiviral Constitutive...expression as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are available....expression ID Plasmid Description PI 11578 pSico Cre addition causes EGFP to be recombined out of the construct...shRNA expression Tyler Jacks 11586 pSico PGK puro Cre addition causes the puromycin gene to be recombined...activating shRNA expression Tyler Jacks 11579 pSicoR Cre addition causes both EGFP and shRNA to be recombined...

...bio 32407 CD200RCd4d3+4-blac 32408 secretedBirA-8his 64395 secreted BirA-Flag...An essential list of plasmids used for AVEXIS screening, including positive and negative controls. Plasmid...AVEXIS is an avidity-based extracellular interaction screen to detect protein interactions, where the extracellular...will need to start producing proteins for AVEXIS screening. It includes negative control bait and preys (... references for more information: Large-scale screening for novel low-affinity extracellular protein interactions...

...tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro 72255...generated infectious virus, plasmids expressing both the Cre and Flp recombinases to remove genes flanked by either... 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1-DIO-tdTomato...72255 NOT AVAILABLE YET Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre...expressing a variety of reporters as well as the Cre and Flp recombinases. Genes can be cloned into these...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express... pAdx-CMV-iCre-p2A-copGFP 73350 Expresses iCre and EGFP from the CMV promoter pAdx-CMV-iCre-P2A-tdTomato...

...in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors...AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre recombinase expression ...pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre recombinase expression ...AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre recombinase expression ...PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...network function. Browse plasmids or viral preps created with support from the BRAIN Armamentarium Project...

...expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional...Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium...High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium sensors for imaging...sensors for imaging neural activity (Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for...imaging neural activity (GCaMP6f/m/s) (Constitutive or Cre-dependent) Cell-specific restoration of stimulus ... of GCaMP6s or GCaMP6f in axons (Constitutive or Cre-dependent) In vivo measurement of afferent activity...GCaMP6s or GCaMP7b sensors fused to synaptophysin (Cre or Flp-dependent) Larsen Lab AAV GCaMP6 Plasmids ...

...63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian ...Conditional overexpression plasmid; deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and...largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized cells (see Weinberg Lab page for...pBABE-hygro-hTERT MoMLV Expression of hTERT for creation of immortalized cells; additional plasmids include...cloning and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE... post-transcriptional regulatory element that increases export to the cytoplasm Hahn 11375 MDH1-PGK-GFP...

... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell...) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with direct...