...Browse the nuclear receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling... Plasmid Collections Nuclear Receptor Plasmid Collection Nuclear Receptor Plasmid Collection You...Engineering Guide Empty-Backbone Resource Image is released to public domain by author. Source: Wikipedia,...Wikipedia, accessed November 6, 2013. Author: Boghog2 Nuclear receptors are a class of transcription factors modulated...modulated by ligands. Two of the important regions of nuclear receptors are: 1) a ligand binding domain, which...activation or repression of target gene expression. Nuclear receptors play a key role in development and homeostasis...from around the globe, Addgene has assembled a Nuclear Receptor plasmid collection for the community. ...

...Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger Consortium: Nuclease Expression Vectors This...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays can...page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created...cloning sites. Vectors encoding wild-type FokI nuclease domains: pST1374 (for expression in mammalian ...in plants) Vectors encoding heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric...

...they can be cleaved out from the larger transcript by the site-specific Csy4 ribonuclease. Vectors expressing...Joung lab Dimeric CRISPR RNA-guided FokI Nuclease (RFN) CRISPR...CRISPR-FokI Dimeric CRISPR RNA-guided FokI Nuclease (RFN) Technology You may also like... CRISPR Guide CRISPR Protocols... practicing the dimeric CRISPR RNA-guided FokI Nuclease ( RFN ) technology developed by the Joung lab ...without registration. pSQT1601 encodes the Csy4 ribonuclease (required for processing of gRNAs co-expressed...which the two components are joined by a T2A self-cleaving peptide sequence. The Csy4 and FokI domains have...

...genomic DNA sequence and a nuclease (e.g.—the Cas9 endonuclease) that cleaves the target DNA. The Joung...CRISPR-Cas9 nucleases with altered PAM specificities Dimeric CRISPR RNA-guided FokI Nucleases (RFNs) CRISPR-Cas...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome...Plasmids Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR...gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type II...II CRISPR-Cas systems, are programmable endonucleases that can be used to perform targeted genome editing...

...like zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required...binding that positions the nuclease domains, called RuvC and HNH, to cleave opposite strands of the target... two nuclease domains, RuvC and HNH. Cas9 nickase, a D10A mutant of SpCas9, retains one nuclease domain... of their ability to cleave target DNA. Specifically, both RuvC and HNH nuclease domains can be rendered...SpCas9), resulting in a nuclease dead Cas9 (dCas9) molecule that cannot cleave target DNA. The dCas9 molecule...enzymes can also cleave RNAs non-specifically after the initial crRNA-guided cleavage. This promiscuous...commonly used CRISPR endonuclease for genome engineering, it may not be the best endonuclease for every application...

...HLALS NFYA nuclear transcription factor Y, alpha CBF-A, CBF-B, FLJ11236, HAP2, NF-YA NFYB nuclear transcription...CST-29 CRH corticotropin releasing hormone CRF CRHR1 corticotropin releasing hormone receptor 1 CRF-R,... growth hormone releasing hormone GHRF, GRF, MGC119781 GHRHR growth hormone releasing hormone receptor... MGC126867 GNRH1 gonadotropin-releasing hormone 1 (luteinizing-releasing hormone) GNRH, GRH, LHRH, LNRH...GNRH2 gonadotropin-releasing hormone 2 GnRH-II, LH-RHII GNRHR gonadotropin-releasing hormone receptor GNRHR1...antimicrobial peptide HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1, PLTR HBEGF heparin-binding EGF-like growth ...NROB1 NR0B2 nuclear receptor subfamily 0, group B, member 2 FLJ17090, SHP, SHP1 NR1D1 nuclear receptor subfamily...

... fused to the N-terminus of monomeric Cerulean mCerulean C1 Cyan Mammalian Express a gene of interest ...Plasmid FRET Pair C5V Cerulean attached to Venus via a 5 amino acid linker C17V Cerulean attached to Venus... acid linker C32V Cerulean attached to Venus via a 32 amino acid linker C5A Cerulean attached to Amber...acid linker C17A Cerulean attached to Amber via a 17 amino acid linker C32A Cerulean attached to Amber...linker-Cerulean-6aa linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa...linker-Cerulean-6aa linker-Amber VCV Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa...Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express a gene of interest fused...

...also promotes non-specific RNA cleavage, but this non-specific cleavage does not occur in mammalian cells...genome engineering technologies like zinc finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune ...Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material, which...The CRISPR complex binds to the foreign DNA and cleaves it to destroy the invader ( Figure 1 ). Figure ...the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique crRNA...Cas9 separates the double stranded DNA target and cleaves both strands near the PAM. (6) The crRNA:tracrRNA... on the structure of the effector complex that cleaves the genomic DNA. Type II CRISPR was the first system...

...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...Recommended Applications PI Additional Resources To learn more, please visit the BRAIN Initiative Resource List ...viral particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...

...tag with TEV cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV cleavage, AmpR 26094 pET28a-LIC...with Thrombin cleavage, KanR 26096 pET28-MHL EF456735 Hexahistidine tag with TEV cleavage, KanR 26098 pCW-LIC...with TEV cleavage, KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106...thioredoxin with TEV cleavage, KanR 26107 pNIC-ZB GU452710 Hexahistidine tag , Z-basic, TEV cleavage, AmpR 26108...pET28a-LIC EF442785 Hexahistidine tag with Thrombin cleavage, KanR 26101 pET28GST-LIC EF456739 GST-tag and ...pFBOH-LIC EF456740 Hexahistidine tag with Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117...pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector...

.../IPTG inducible) N terminal, TEV cleavable 6x-His tag Ophear Gileadi pET-28-a based expression vector ...Luciano Marraffini Bacterial expression of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial...TALEN fused to an E. coli codon optimized FokI endonuclease domain. pET-Sangamo-His 40787 TALEN Gang Bao...TALEN fused to an E. coli codon optimized FokI endonuclease domain. Return to Top Visualization and Tagging...in bacteria, add epitope tags to it, and, later, cleave those tags from the purified protein. Protein purification...challenging, but once you get it right, you can learn many things about your protein’s function, structure...lactose/IPTG inducible) N terminal GST tag with TEV cleavage site to remove tag Nicola Burgess Brown pGEX expression...

...glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease I 165551 pOpen-Endonuclease IV E. coli E... E. coli Endonuclease IV 165561 pOpen-Endonuclease VIII E. coli E. coli Endonuclease VIII 165571 pOpen-Exonuclease...pOpen-Exonuclease III E. coli E. coli Exonuclease III 165559 pOpen-T5gene12/13 T5 Exonuclease 165560 pOpen-T4...Collection DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...below: DNA Ligases DNA Polymerases Restriction Endonucleases Reverse Transcriptases RNA Polymerases Other...phi29 DNA Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-...

...mCherry-LaminA-C-18 Nuclear Envelope LaminA1 mCherry* Michael Davidson 98831 mScarlet-I-LaminB Nuclear Envelope ...mTurquoise2-LaminB Nuclear Envelope LaminB mTurquoise2 Dorus Gadella 55069 mCherry-LaminB1-10 Nuclear Envelope ...nucleoplasm None dL5 FAP; mCerulean3 Marcel Bruchez 36206 pmTurquoise2-NES Non-nucleus Nuclear Export Sequence ...Non-nucleus Nuclear Export Sequence mScarlet-I Dorus Gadella 85061 pmScarlet-H_NES_C1 Non-nucleus Nuclear Export...sequence and COX VIII signal peptide dL5 FAP; mCerulean3 Marcel Bruchez 44385 pLV-mitoGFP Mitochondria...2xG4S-mCer3 Nucleus NLS (from Mak16p protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3...Dorus Gadella 85060 pmScarlet_NES_C1 Non-nucleus Nuclear Export Sequence mScarlet Dorus Gadella 37351 pQC...

...DS-SPcasN- Bacterial nuclease-null SP Cas9 expression 48659 DS-ST1casN- Bacterial nuclease-null ST1 Cas9 expression...expression of a Cas9 protein bearing a C terminus SV40 nuclear localization signal with one or more guide RNAs... III promoter. Cas9 unwinds the DNA duplex and cleaves both strands upon recognition of a target sequence...above Cas9 protein, NM is comparably active as a nuclease and a transcriptional activator. Streptococcus...slightly higher activities than the others as a nuclease and as an activator, but is more restricted in...transcriptional repressor. All genes except the SP nuclease are human codon optimized but function well in...SP and ST1. Protospacer-adjacent motifs (PAMs): Please note that NM, ST1, and TD recognize different PAMs...

...Expression Cerulean 433 475 27 4.7 Monomer (A206K) Cerulean - Mammalian Expression pCS2+8CCerulean - Zebrafish...Expression mCerulean C1 - Mammalian Expression mCerulean3 433 475 32 3.2 Monomer (A206K) mCerulean3-N1 - Mammalian...Expression mCerulean3-C1 - Mammalian Expression moxCerulean3 434 474 36 3.2 Monomer (A206K) moxCerulean3 - Mammalian...C.elegans/Sea urchin pCS2+8NCerulean - Zebrafish/Xenopus/C.elegans/Sea urchin mCerulean N1 - Mammalian Expression...Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many N- and C-terminal... Plasmids - Set includes tagging with mCherry, Cerulean, Citrine, and eGFP pPD95_75 - C-terminal GFP for...cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia Lab Bacterial Vectors - Includes...

...Looking For? Please let us know what you would like to see available to industry scientists. Please note this...page for more information. To learn more about Addgene’s MTA process please visit our Help Center . The ...collection of plasmids that are available to industry. Learn more about the available plasmids and how to order...Addgene as well as by our trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended...account, you can place orders through our website. Please note that all orders must be placed online through... product pages after you login to your account. Please see our Help Center for more information on pricing...language which we are allowed to use for distribution. Please see the Sample Agreements on our Technology Transfer...

...Looking For? Please let us know what you would like to see available in viral format. Please note this does...available in each of the above serotypes and buffers. Please click on the plasmid name for ordering information...the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite Petrs-Silva...AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye, et al. ...AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite Ling, et al...using the AAV44.9 serotype in future publications, please acknowledge John Chiorini and Shannon Boye and ...

...an order, please email [email protected] with the plasmid catalog ID and AAV serotype. Please note that... resume, but we will post updates on this page. Please email [email protected] if you have any questions...AAV Made on Request is an Addgene Beta service. Learn more about Addgene's Beta Projects and how you can...further notice. Shipping Price varies by location. Please contact us for more information. Standard Quality...services. To find out more about a specific plasmid, please email [email protected] Do I need to provide the...available on our website. How can I get a price quote? Please email [email protected] . I submitted an AAV Made...and to determine if viral production is feasible. Please note that we cannot guarantee legal approval to...

...have final approval before anything is released publicly, but please only share materials that you are comfortable...Learn about Addgene's 2018 AAV Education and Development (AAVED) workshop series. The...Leila Haery from Addgene will also be speaking or leading workshops. Tentative Meeting Agenda Thursday, September...examples of AAV strategies for intersectional control. Please see our list of suggested materials below. Suggested...Detailed protocol: AAV Production in HEK293 Cells Please share materials by uploading to your Dropbox link...comfortable and willing to share. Please only share materials that you have permission to share (e.g.,...contributions and your commitment to open science! Please see the meeting preparation section for how to ...

...partnership and the plasmids that are available, please see the Ginkgo Bioworks Blog post: Synthesizing...information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...entry into cells. ( 2 ) FURIN - an enzyme that cleaves precursor proteins to a biologically active state... The SARS-CoV-2 S protein contains a potential cleavage site for furin proteases.( 3 ) BSG - (CD147), ...function.( 5 ) TACE - (ADAM17) a metalloprotease that cleaves hACE2, potentially increasing viral infectivity...degradation to develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth...resources, such as reagents, data, news, and more, please see Ginkgo's Synthetic Biology (and beyond) Community...