...times (over 800 orders in 2017) and expresses the VSV-G envelope protein under the control of the powerful...determine the types of cells that it can infect. The VSV-G envelope is widely used and requested because it... types of cells. This means researchers who use VSV-G can deliver their genes of interest to a variety...and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence... vsv-g...envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been...pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad usefulness of pMD2.G and...

...Broad tropism: VSV-G pseudotyping A commonly used envelope protein for pseudotyping is VSV-G, or vesicular...one of the reasons VSV-G is used to transduce stem cells and neurons. However, VSV-G’s broad tropism can...vesicular stomatitis virus G protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-density...cytotoxicity can vary. For example, the popular VSV-G envelope protein causes cells that it infects to...-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated by serum regardless...surface to endocytose into the cell. The reason VSV-G is so commonly used is that it is good at infecting...and B cells is low. Infection of these cells with VSV-G pseudotyped virus requires the upregulation of LDLR...

...all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing...Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab and...determine which types of cells a virus can infect. The VSV-G envelope protein enables this virus to infect many... plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging... was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from the...pMDLg/pRRE and the envelope expressing plasmid pMD2.G, both deposited from the same lab. Find pRSV-Rev. ...pRSV-Rev and the envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from...

...Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope...pseudotyped to alter infectivity): The use of the VSV-G envelope provides the widest tropism or range of...interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however...

...Hoyah G, Winemiller A, Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas...different viral envelope protein (Env), usually from VSV-G due to wide infectivity, is often used as a substitute...requested plasmids! Lentiviral particles with the VSV-G envelope have high infectivity in a wide range of...β-thalassaemia. Cavazzana-Calvo M, Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, Westerman... Larghero J, Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta...

...Lentiviral vectors are pseudotyped in most cases with VSV-G that generally mediates broad cell tropism and has...behavioral studies. References Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer to the CNS using recombinant...

...the lentiviral production and packaging vectors VSV-G and psPAX2, which were used in a publication by ...

...found that Nanoblades pseudotyped with a mix of VSV-G and the baboon endogenous retrovirus Rless glycoprotein...

...pseudotyping. Lentiviruses pseudotyped with the VSV-G envelope protein use the LDL receptor to infect ... B., Metharom P., Jullie H., Ellem K.A., Cleghorn G., West M.J., Wei MQ. “The significance of controlled...

...plasmids; an envelope plasmid that usually encodes VSV-G, a packaging plasmid containing structural genes...

...interchangeable. General envelope plasmids, such as VSV-G, may be used across both systems. Below I provide...

...of envelope dictates the tropism of the virus.  VSV-G is very common due to its broad host range, and ...

...plasmid), and 3) the vesicular stomatitis virus G protein (VSV-G) that improves the stability and broadens ...

...pseudorabies virus type 1, vesicular stomatitis virus (VSV; Beier et al., 2011), adenovirus, and adeno-associated...F., Hisey, E., Tanaka, M., Kearney, M., Chattree, G., Yang, C. F., Shah, N.M., and Mooney, R. (2017). ...Salegio, E.A., Samaranch, L., Kells, A.P., Mittermeyer, G., San Sebastian, W., Zhou, S., Beyer, J., Forsayeth...Ther 17, 141–146. PubMed PMID: 19727139. Ugolini, G., Kuypers, H.G., and Strick, P.L. (1989). Transneuronal...

...expression of the vesicular somatitis virus G glycoprotein (VSVG). VSVG is a broad tropism envelope protein that...