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We narrowed to 24 results for: vsv-g

Showing: 1 - 20 of 24 results

  1. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...times (over 800 orders in 2017) and expresses the VSV-G envelope protein under the control of the powerful...determine the types of cells that it can infect. The VSV-G envelope is widely used and requested because it... types of cells. This means researchers who use VSV-G can deliver their genes of interest to a variety...and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence... vsv-g...envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been...pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad usefulness of pMD2.G and...

  2. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    ...Weinberg pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein...: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope...applicable export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks...Akitsu Hotta pHCMV-RabiesG Contains Rabies virus G protein; see depositing paper for more information...

  3. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Broad tropism: VSV-G pseudotyping A commonly used envelope protein for pseudotyping is VSV-G, or vesicular...one of the reasons VSV-G is used to transduce stem cells and neurons. However, VSV-G’s broad tropism can...vesicular stomatitis virus G protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-density...cytotoxicity can vary. For example, the popular VSV-G envelope protein causes cells that it infects to...-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated by serum regardless...surface to endocytose into the cell. The reason VSV-G is so commonly used is that it is good at infecting...and B cells is low. Infection of these cells with VSV-G pseudotyped virus requires the upregulation of LDLR...

  4. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing...Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab and...determine which types of cells a virus can infect. The VSV-G envelope protein enables this virus to infect many... plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging... was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from the...pMDLg/pRRE and the envelope expressing plasmid pMD2.G, both deposited from the same lab. Find pRSV-Rev. ...pRSV-Rev and the envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from...

  5. Retrovirus Plasmids

    Type
    Collection
    ...pCMV-VSV-G Envelope Envelope protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing...35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis and Salmon 22501 pHEF-VSVG Envelope ...VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG Envelope VSV-G recommended for the FELIX (FIV-based) system...Envelope VSV-G-expressing envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral...

  6. Lentivirus Plasmids

    Type
    Collection
    ... pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing...35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis and Salmon 17532 pLTR-G 2nd Envelope...generation transfer plasmids Weinberg 1733 pCI-VSVG N/A Envelope VSV-G recommended for the FELIX (FIV-based) system...increases viral titer Kasparov 22501 pHEF-VSVG N/A Envelope VSV-G-expressing envelope plasmid Kasparov 22500...Envelope Expression of VSV-G glycoprotein (Indiana strain). Use with packaging construct encoding Tat. Reiser...

  7. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope...pseudotyped to alter infectivity): The use of the VSV-G envelope provides the widest tropism or range of...interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Hoyah G, Winemiller A, Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas...different viral envelope protein (Env), usually from VSV-G due to wide infectivity, is often used as a substitute...requested plasmids! Lentiviral particles with the VSV-G envelope have high infectivity in a wide range of...β-thalassaemia. Cavazzana-Calvo M, Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, Westerman... Larghero J, Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta...

  10. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...with most lentiviral vectors being packaged using VSV-G. In contrast, AAV can be packaged in a variety of...E., Kuhn, M., Farouni, R., Lagler, S., Kalamakis, G., Lischetti, U., Jabaudon, D., & Platt, R. J. (2023...Gessler, D. J., Zhan, W., Gallagher, T. L., & Gao, G. (2024). Adeno-associated virus as a delivery vector...

  12. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...typically encodes the vesicular stomatitis virus G protein (VSV-G), which facilitates viral entry into the host...Central PMCID: PMC2496964 Leavitt, A. D., Robles, G., Alesandro, N., & Varmus, H. E. (1996). Human immunodeficiency...

  14. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...pseudotyping. Lentiviruses pseudotyped with the VSV-G envelope protein use the LDL receptor to infect ... B., Metharom P., Jullie H., Ellem K.A., Cleghorn G., West M.J., Wei MQ. “The significance of controlled...

  19. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...pseudorabies virus type 1, vesicular stomatitis virus (VSV; Beier et al., 2011), adenovirus, and adeno-associated...F., Hisey, E., Tanaka, M., Kearney, M., Chattree, G., Yang, C. F., Shah, N.M., and Mooney, R. (2017). ...Salegio, E.A., Samaranch, L., Kells, A.P., Mittermeyer, G., San Sebastian, W., Zhou, S., Beyer, J., Forsayeth...Ther 17, 141–146. PubMed PMID: 19727139. Ugolini, G., Kuypers, H.G., and Strick, P.L. (1989). Transneuronal...

  20. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...gene env ; usually VSV-G due to wide infectivity VSV-G (vesicular stomatitis virus G protein) is the most...gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein with broad tropism...the envelope gene, a process called pseudotyping. VSV-G is by far the most common envelope gene, as it has... the stability of the viral particles. However, VSV-G is toxic under long-term, constitutive expression...expression. In order to make stable cell lines, VSV-G is often put under an inducible promoter. Depending on ...27097319 LaFave, M. C., Varshney, G. K., Gildea, D. E., Wolfsberg, T. G., Baxevanis, A. D., & Burgess, S...Choi, U., Macpherson, J. L., Poidinger, M., Symonds, G., Pond, S. M., Ferris, A. L., Hughes, S. H., Malech...
Showing: 1 - 20 of 24 results