...times (over 800 orders in 2017) and expresses the VSV-G envelope protein under the control of the powerful...determine the types of cells that it can infect. The VSV-G envelope is widely used and requested because it... types of cells. This means researchers who use VSV-G can deliver their genes of interest to a variety...and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence... vsv-g...envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been...pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad usefulness of pMD2.G and...

...Broad tropism: VSV-G pseudotyping A commonly used envelope protein for pseudotyping is VSV-G, or vesicular...one of the reasons VSV-G is used to transduce stem cells and neurons. However, VSV-G’s broad tropism can...vesicular stomatitis virus G protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-density...cytotoxicity can vary. For example, the popular VSV-G envelope protein causes cells that it infects to...-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated by serum regardless...surface to endocytose into the cell. The reason VSV-G is so commonly used is that it is good at infecting...and B cells is low. Infection of these cells with VSV-G pseudotyped virus requires the upregulation of LDLR...

...all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing...Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab and...determine which types of cells a virus can infect. The VSV-G envelope protein enables this virus to infect many... plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging... was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from the...pMDLg/pRRE and the envelope expressing plasmid pMD2.G, both deposited from the same lab. Find pRSV-Rev. ...pRSV-Rev and the envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from...

...pCMV-VSV-G Envelope Envelope protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing...35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis and Salmon 22501 pHEF-VSVG Envelope ...VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG Envelope VSV-G recommended for the FELIX (FIV-based) system...Envelope VSV-G-expressing envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral...

... pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing...35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis and Salmon 17532 pLTR-G 2nd Envelope...generation transfer plasmids Weinberg 1733 pCI-VSVG N/A Envelope VSV-G recommended for the FELIX (FIV-based) system...increases viral titer Kasparov 22501 pHEF-VSVG N/A Envelope VSV-G-expressing envelope plasmid Kasparov 22500...Envelope Expression of VSV-G glycoprotein (Indiana strain). Use with packaging construct encoding Tat. Reiser...

...Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope...pseudotyped to alter infectivity): The use of the VSV-G envelope provides the widest tropism or range of...interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however...

...Hoyah G, Winemiller A, Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas...different viral envelope protein (Env), usually from VSV-G due to wide infectivity, is often used as a substitute...requested plasmids! Lentiviral particles with the VSV-G envelope have high infectivity in a wide range of...β-thalassaemia. Cavazzana-Calvo M, Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, Westerman... Larghero J, Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta...

...Lentiviral vectors are pseudotyped in most cases with VSV-G that generally mediates broad cell tropism and has...behavioral studies. References Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer to the CNS using recombinant...

...the lentiviral production and packaging vectors VSV-G and psPAX2, which were used in a publication by ...

...found that Nanoblades pseudotyped with a mix of VSV-G and the baboon endogenous retrovirus Rless glycoprotein...

...pseudotyping. Lentiviruses pseudotyped with the VSV-G envelope protein use the LDL receptor to infect ... B., Metharom P., Jullie H., Ellem K.A., Cleghorn G., West M.J., Wei MQ. “The significance of controlled...

...plasmids; an envelope plasmid that usually encodes VSV-G, a packaging plasmid containing structural genes...

...interchangeable. General envelope plasmids, such as VSV-G, may be used across both systems. Below I provide...

...of envelope dictates the tropism of the virus.  VSV-G is very common due to its broad host range, and ...

...plasmid), and 3) the vesicular stomatitis virus G protein (VSV-G) that improves the stability and broadens ...

...pseudorabies virus type 1, vesicular stomatitis virus (VSV; Beier et al., 2011), adenovirus, and adeno-associated...F., Hisey, E., Tanaka, M., Kearney, M., Chattree, G., Yang, C. F., Shah, N.M., and Mooney, R. (2017). ...Salegio, E.A., Samaranch, L., Kells, A.P., Mittermeyer, G., San Sebastian, W., Zhou, S., Beyer, J., Forsayeth...Ther 17, 141–146. PubMed PMID: 19727139. Ugolini, G., Kuypers, H.G., and Strick, P.L. (1989). Transneuronal...

...Interchangeable: usually encodes for VSV-G Interchangeable: usually encodes for VSV-G Safety Safe. Replication incompetent...activate expression. The envelope protein Env (usually VSV‐G due to its wide infectivity) is encoded on a third...interact with receptors at the host cell surface. The VSV-G envelope protein is commonly used in lentiviral ...to transgene Purpose Envelope VSVG in trans Vesicular stomatitis virus G glycoprotein; Broad tropism envelope...

...pseudotyped to alter infectivity): The use of the VSV-G envelope provides the widest tropism or range of...interchangeable. General envelope plasmids, such as VSV-G, however, may be used across both systems. Please...as Transgene? Purpose Envelope VSVG in trans Vesicular somatitis virus G glycoprotein; Broad tropism envelope...

...expression of the vesicular somatitis virus G glycoprotein (VSVG). VSVG is a broad tropism envelope protein that...

... A, G, or T H A, C, or T K G or T M A or C N A, T, C, or G R A or G S C or G V A, C, or G W A or T Y C...) and Guanine (G). In the double helix A always pairs with T and C always pairs with G. RNA on the other... Cytosine G Guanine T Thymine U Uracil Single Letter Code: Ambiguous bases Nucleobase B C, G, or T D A...cytosine, and guanine (abbreviated to A, T, C, and G respectively) that are organized into a double stranded...strand. Specifically, A pairs with T and C pairs with G. During replication, DNA unwinds and the DNA polymerase... DNA strand in addition to the normal A, T, C, or G nucleotide. The 4 standard bases are tagged with a...time a base is added. Again, each base (A,C,T, or G) is labelled with a different color making it easy...