...aggregation, which they reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1. In SunTag nomenclature...throughout the rest of this blog post, GFP refers to sfGFP-GB1.   Tanenbaum et al. examined the power of SunTag...-targeted CAAX-SunTag was 18-fold brighter than sfGFP! The high SunTag signal allowed them to cut their...

...Hu CD. Biotechniques. 2006 Chang-Deng Hu Green sfGFP Split super-folder GFP, our most-requested GFP(1... variant of mScarlet Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of...

...second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter known to bind...

...generation of iGluSnFR, the Looger lab swapped eGFP for sfGFP to create SF-iGluSnFR. This improved variant has...

...translated to a few hours. For example, superfolder GFP (sfGFP) and mNeonGFP can fold in <10min at 37°C, mCherry...

...fluorescently labeled by scFv antibodies fused to sfGFP. This system amplifies the intensity of fluorescence...

...stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides...