Bikard Lab - CRISPR Repression Collection
Type
Collection
...carrying two CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides...for these two reporters simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus ...sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐...