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Showing: 1 - 14 of 14 results

  1. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...aggregation, which they reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1. In SunTag nomenclature...throughout the rest of this blog post, GFP refers to sfGFP-GB1.   Tanenbaum et al. examined the power of SunTag...-targeted CAAX-SunTag was 18-fold brighter than sfGFP! The high SunTag signal allowed them to cut their...

  3. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...carrying two CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides...for these two reporters simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus ...sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression sfGFP 485 507 54 Prone to dimerization sfGFP-N1 - Mammalian Expression sfGFP-C1 - Mammalian...Expression PA-sfGFP 485 511 Prone to dimerization PA-sfGFP-N1 - Mammalian Expression PA-sfGFP-C1 - Mammalian...Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510 50 Monomer (A206K) moxGFP - Mammalian...

  8. Cre-lox system

    Type
    Collection
    ...pCAG-sfGFP-GSAx9-iCre-ERT2 iCre-ERT2 - Tamoxifen inducible CAG Mammalian Capecchi 125749 pCAG-sfGFP-iCre-ERT2...Lentiviral Kotton 128166 pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2 sfGFP-iCre-ERT2 (PAPGSTM N-terminus, unskippable... Capecchi 128167 pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2 sfGFP-iCre-ERT2 (PAPGSTM N-terminus, GSAx9 ...

  9. Genetic Code Expansion

    Type
    Collection
    ...C321.Ub-UAG-sfGFP all TAG sites changes to UAG, RF1 function removed, with Ubiquitin-UAG-sfGFP reporter ...reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all TAG sites changes to UAG, RF1 function removed, ClpS...ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG codons...

  10. Antibody Plasmid Collection

    Type
    Collection
    ...peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Martens 209078 piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120) SNCA GFP TRE Parkinson'...Khurana 209080 piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123) SNCA GFP TRE Parkinson'... 209081 piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124) SNCA GFP TRE Parkinson'...
Showing: 1 - 14 of 14 results