We narrowed to 14 results for: sfGFP
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TypeBlog Post...aggregation, which they reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1. In SunTag nomenclature...throughout the rest of this blog post, GFP refers to sfGFP-GB1. Tanenbaum et al. examined the power of SunTag...-targeted CAAX-SunTag was 18-fold brighter than sfGFP! The high SunTag signal allowed them to cut their...
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Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...Hu CD. Biotechniques. 2006 Chang-Deng Hu Green sfGFP Split super-folder GFP, our most-requested GFP(1... variant of mScarlet Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter known to bind... -
Bikard Lab - CRISPR Repression Collection
TypeCollection...carrying two CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides...for these two reporters simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus ...sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...generation of iGluSnFR, the Looger lab swapped eGFP for sfGFP to create SF-iGluSnFR. This improved variant has... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...Mammalian Expression sfGFP 485 507 54 Prone to dimerization sfGFP-N1 - Mammalian Expression sfGFP-C1 - Mammalian...Expression PA-sfGFP 485 511 Prone to dimerization PA-sfGFP-N1 - Mammalian Expression PA-sfGFP-C1 - Mammalian...Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510 50 Monomer (A206K) moxGFP - Mammalian... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...translated to a few hours. For example, superfolder GFP (sfGFP) and mNeonGFP can fold in <10min at 37°C, mCherry... -
Cre-lox system
TypeCollection...pCAG-sfGFP-GSAx9-iCre-ERT2 iCre-ERT2 - Tamoxifen inducible CAG Mammalian Capecchi 125749 pCAG-sfGFP-iCre-ERT2...Lentiviral Kotton 128166 pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2 sfGFP-iCre-ERT2 (PAPGSTM N-terminus, unskippable... Capecchi 128167 pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2 sfGFP-iCre-ERT2 (PAPGSTM N-terminus, GSAx9 ... -
Genetic Code Expansion
TypeCollection...C321.Ub-UAG-sfGFP all TAG sites changes to UAG, RF1 function removed, with Ubiquitin-UAG-sfGFP reporter ...reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all TAG sites changes to UAG, RF1 function removed, ClpS...ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG codons... -
Antibody Plasmid Collection
TypeCollection...peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...mCherry Robin Shaw 69007 Cx43-msfGFP Gap Junctions Connexin 43 / GJA1 sfGFP David Spray 54959 mApple-VE-... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...fluorescently labeled by scFv antibodies fused to sfGFP. This system amplifies the intensity of fluorescence... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides... -
Neurodegeneration Plasmid Collection
TypeCollection...Martens 209078 piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120) SNCA GFP TRE Parkinson'...Khurana 209080 piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123) SNCA GFP TRE Parkinson'... 209081 piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124) SNCA GFP TRE Parkinson'...