Visualizing Genomic Loci with CRISPR-Sirius
Type
Blog Post
Published
April 18, 2019, 12:53 p.m.
...Figure 2: CRISPR-Sirius tricolor labeling. (A) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius-8XPP7, and sgRNA-Sirius...Developing CRISPR-Sirius
Recently, Hanhui Ma and the Thoru Pederson lab developed CRISPR-Sirius (Ma et al., ...needed to deliver CRISPR-Sirius into cells. They named this guide sgRNA-Sirius-8XMS2 which will recruit...(sgRNA-Sirius-8XPP7) to recruit GFP to the loci of interest. To determine how the sgRNA-Sirius-8XPP7 and...sgRNA-Sirius-4X(MS2-PP7) showing recruitment of different fluorescent proteins. (B) CRISPR-Sirius used ...CRISPR-Sirius?
With all the tools out there to visualize genomic loci, what makes CRISPR-Sirius unique...genomic loci brightly, give CRISPR-Sirius a try.
Find CRISPR-Sirius plasmids at Addgene.
References
Ma...