...Figure 2: CRISPR-Sirius tricolor labeling. (A) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius-8XPP7, and sgRNA-Sirius...Developing CRISPR-Sirius Recently, Hanhui Ma and the Thoru Pederson lab developed CRISPR-Sirius (Ma et al., ...needed to deliver CRISPR-Sirius into cells. They named this guide sgRNA-Sirius-8XMS2 which will recruit...(sgRNA-Sirius-8XPP7) to recruit GFP to the loci of interest. To determine how the sgRNA-Sirius-8XPP7 and...sgRNA-Sirius-4X(MS2-PP7) showing recruitment of different fluorescent proteins. (B) CRISPR-Sirius used ...CRISPR-Sirius? With all the tools out there to visualize genomic loci, what makes CRISPR-Sirius unique...genomic loci brightly, give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma...

...Maturation Structure Plasmids Sirius 355 424 4 3.0 Prone to dimerization Sirius-N1 - Mammalian Expression ...

...better imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for...Grunwald, D., Zhang, S., & Pederson, T. (2018). CRISPR-Sirius: RNA scaffolds for signal amplification in genome...

...better imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for...Grunwald, D., Zhang, S., & Pederson, T. (2018). CRISPR-Sirius: RNA scaffolds for signal amplification in genome...