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Plasmid#121944PurposeCRISPR-Sirius plasmidDepositorInserthU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6 promotersAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sirius/pRSETB
Plasmid#51956Purposebacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent proteinDepositorInsertSirius
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sirius/pcDNA3
Plasmid#51957Purposemammalian expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent proteinDepositorInsertSirius
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sirius-N1
Plasmid#54506PurposeLocalization: N1 Cloning Vector, Excitation: 355, Emission: 424DepositorTypeEmpty backboneUseTagsSiriusExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sirius-mT-Sapphire_pcDNA3
Plasmid#89584PurposeGenetically encoded ratiometric fluorescent thermometerDepositorInsertSirius-mT-Sapphire
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sirius-H2B-C-10
Plasmid#55226PurposeLocalization: Nucleus/Histones, Excitation: 355, Emission: 424DepositorInsertH2B (H2BC11 Human)
UseTagsSiriusExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Sirius-H2B-6
Plasmid#55225PurposeLocalization: Nucleus/Histones, Excitation: 355, Emission: 424DepositorInsertH2B (H2BC11 Human)
UseTagsSiriusExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFX-β-tubulin-Sirius
Plasmid#175825PurposeExpress Sirius with microtubule-targeting sequence in mammalian cellsDepositorInsertSirius with microtuble-targeting sequence
UseTagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-H2B-Sirius
Plasmid#175837PurposeExpress Sirius with nucleus-targeting sequence in mammalian cellsDepositorInsertSirius with nucleus-targeting sequence
UseTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Sirius-KRasCT
Plasmid#175831PurposeExpress Sirius with plasma membrane-targeting sequence in mammalian cellsDepositorInsertSirius with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-Sirius
Plasmid#174475PurposeExpress Sirius with actin-targeting sequence in mammalian cellsDepositorInsertSirius with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-Sirius
Plasmid#174184PurposeExpress Sirius with mitochondria-targeting sequence in mammalian cellsDepositorInsertSirius with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Sirius-Sec61β
Plasmid#174464PurposeExpress Sirius with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertSirius with endoplasmic reticulum-targeting sequence
UseTagsSec61 subunit betaExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-GalT-Sirius
Plasmid#174469PurposeExpress Sirius with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertSirius with golgi apparatus-targeting sequence
UseTagsβ1,4-galactose transferaseExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-8XMS2
Plasmid#121939PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-8XPP7
Plasmid#121940PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPUR-hU6-sgRNA-Sirius-8XMS2
Plasmid#121942PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPUR-mU6-sgRNA-Sirius-8XPP7
Plasmid#121943PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-4X(MS2-PP7)
Plasmid#121941PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-4X(MS2-PP7)
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only