Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
Type
Blog Post
Published
July 23, 2019, 12:46 p.m.
...CNS transduction and transduces the majority of astrocytes and neurons across multiple CNS regions. PHP....discovered as an antigen upregulated on activated lymphocytes and is commonly used to enrich adult mouse hematopoietic...splicing, or protein coding regions. In total, they tested 13 strains, with seven strains identified as permissive...in the mouse brain.
Both labs then took similar routes to verify LY6A’s role as a receptor for PHP.B. ...LY6A’s expression affected PHP.B’s transduction rates. Knock out of Ly6a in primary C57BL/6J brain endothelial...endothelial cells decreased transduction rates while overexpression of the C57BL/6J version of Ly6a in ...in 293T cells enhanced PHP.B transduction rates. The group also determined the effect of mice strain specific...