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We narrowed to 818 results for: tes

Showing: 601 - 620 of 818 results
  1. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...sequence containing transcription factor binding sites can increase transgene expression and transduction...validate ITR sequences as these enzymes cut at known sites within the ITR. However, small deletions and point...
  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...site of interest, which nCas9 nicks and then dissociates from. PolI3M then binds the nicked DNA, and extends..., where it nicks its target DNA (left). Cas9 dissociates and PolI binds (middle) and extends from the ...
  3. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... and have worked closely with us to functionally test our virus. Ed Boyden’s lab from MIT and Alla Karpova... methods, performs extensive quality control, generates  educational materials, and provides viral vector...
  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...and isolated with antibiotic selection. The team tested this system in vitro and in vivo. They identified...fluorescent proteins into one bright particle that segregates into one cell during cell division. To achieve...
  5. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...theaters, the Razr was the hottest phone on the market, and two new websites, Facebook and Gmail, were ...
  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally high enough for this approach... guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik....
  7. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...my ability to tell. But even from these rough estimates, it’s clear that community engagement with and...
  8. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...load The Wikipedia definition of cognitive load states: “cognitive load refers to the used amount of working...even the most enthusiastic of learners.  What contributes to cognitive load?  Everything! Here are some...
  9. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...idea of the basics of immunoimaging. A scientist conjugates an antibody with a signaling molecule, the antibody...secondary antibodies on hand and use them to easily test different antibodies or different signaling molecules...
  10. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...weeks to increase the antibody titer. The titer is tested following each immunization until the desired level...
  11. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...(Japanese, German, and English) and even on 60 Minutes. We are blessed with a large community of scientists... employee pool and we get a lot of successful candidates from internal referrals. Our long reaching reputation...
  12. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...below): 1. Resistant cells among the separate replicates would contain the same labels - i.e. they would...subpopulation 2. Resistant cells among the separate replicates would have different labels - i.e. they would...
  13. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...immunogenicity of AdVs is what makes them ideal candidates for applications in oncolysis and vaccination...by recombination. It is therefore recommended to test for the presence of RCA in the viral stock by doing...
  14. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other plasmids...undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be small and poorly ...
  15. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...transfer plasmid, the component that physically integrates into the host genome, the virus is replication...decontaminating liquid waste, remember that the percentage indicates the proportion of the decontaminate in the waste...
Showing: 601 - 620 of 818 results