We narrowed to 815 results for: tes
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TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m.... to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing it to be... customizing gene expression. Sahoo et al. have tested the system with genes up to 4 kb in length and ...
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Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog PostPublishedAug. 11, 2015, 2:30 p.m...., or how organization may be altered in disease states. dCas9 gets a colorful makeover Even without its... cross-talk with the other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....a blank white slate and developed this over 30 minutes, which was fun to watch. A simpler 1 protein -... is already modifying cell and animal genomes to test hypotheses of disease. They are finding they have... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....until now, you’ve always had to call the United States - Cambridge, MA to be exact. Today, that changes...expression of an unknown protein in macrophages/monocytes using lentiviral expression vectors. I also used... -
New Viral Vectors - Fall 2024
TypeBlog PostPublishedOct. 3, 2024, 1:15 p.m....repository Name Serotype Category Depositor Notes AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog PostPublishedOct. 3, 2017, 12:11 p.m....pages, performance data, protocols, and even certificates of analysis.” Han’s biggest piece of advice... at eScience Labs, an educational company that creates hands-on science lab kits and digital curriculum... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog PostPublishedMay 18, 2017, 2:30 p.m.... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical...Biosensor Experiments Experiment Detection Method Notes Quantification of a process Plate-based assays... -
Cellosaurus: A Cell Line Information Database
TypeBlog PostPublishedFeb. 21, 2023, 2:15 p.m....: Periodic surveying of cell line collections websites and catalogues Literature searches utilizing Google...correct one!) Fig. 1: HeLa cells. Image courtesy of Josef Reischig. Contributing to Cellosaurus... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....a conformational change of the receptor which modulates the fluorescence intensity of the cpGFP without... vitro, and they further demonstrated that when tested in tobacco protoplasts, the enzymatic biosensor... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...recommended for use in western blot, but we are actively testing it in other applications! If you try it out in ... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m....with the article metadata, legend and user created notes on a “Pinterest for scientists”? ReFigure extension...profile. To see how to create ReFigures in a few minutes, visit our YouTube Channel. Currently we can only... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m.... four new plasmid pages that cover some of the hottest CRISPR tools. Base Edit: Base editing plasmids...Plan Your Experiment. #12DaysofCRISPR and blog updates We hope you enjoyed the #12DaysofCRISPR, a celebration... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m....microbiology with multiple high-profile publications. My classmates consisted of graduate students, post-grads, and...manuscript is like generating a quilt with your lab mates, you add the individual figures and results to make... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog PostPublishedNov. 14, 2016, 3:30 p.m....requested from Addgene many times as the technology disseminates throughout the research community. These popular...level system on our website: A yellow flame indicates that a plasmid has been requested 20+ times ... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....stayed to see the Opening Keynote address with Bill Gates. The long line for bag check was definitely worth...for global health and the various initiatives the Gates’ Foundation is involved with was one of the highlights... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m.... these meetings in relatively isolated resorts creates a sense of camaraderie with fellow attendees from...tackling this type of genetic engineering is a testament to how amazing the downstream payoff could be.... -
New Viral Vectors - Winter 2025
TypeBlog PostPublishedJan. 28, 2025, noon...repository Name Serotype Category Depositor Notes pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog PostPublishedNov. 8, 2018, 3:47 p.m.... was natural or synthetic 97.7% of the time in a test set of 173 known synthetic and natural genes. ...saw that a percent sequence identity below 85% correlates with synthetic sequences. The researchers then... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m.... described by Li ZY, et al. PLoS One, 2016) and tested a series of mutations. Through this process mCarmine... mNeptune684, and has a favorable pKa of 5.6. To test out this new FP in your own experiments, mCarmine... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....fields of biology and enable the conversion of RNA templates to DNA but suffer from several important shortcomings...faithfully transcribes RNA to DNA with low error rates compared to other RTs. MarathonRT represents a major...