We narrowed to 818 results for: tes

...pyogenes system does not work well in Caulobacter). To test their CRISPRi system, the Laub lab repressed ctrA...that the levels of ctrA mRNA decreased after 20 minutes in xylose (which controls expression of dCas9)....

... to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing it to be... customizing gene expression. Sahoo et al. have tested the system with genes up to 4 kb in length and ...

..., or how organization may be altered in disease states. dCas9 gets a colorful makeover Even without its... cross-talk with the other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric...

...a blank white slate and developed this over 30 minutes, which was fun to watch. A simpler 1 protein -... is already modifying cell and animal genomes to test hypotheses of disease. They are finding they have...

...until now, you’ve always had to call the United States - Cambridge, MA to be exact. Today, that changes...expression of an unknown protein in macrophages/monocytes using lentiviral expression vectors. I also used...

...pages, performance data, protocols, and even certificates of analysis.” Han’s biggest piece of advice... at eScience Labs, an educational company that creates hands-on science lab kits and digital curriculum...

... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical...Biosensor Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays...

...repository  Name Serotype Category Depositor  Notes AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...recommended for use in western blot, but we are actively testing it in other applications! If you try it out in ...

...: Periodic surveying of cell line collections websites and catalogues Literature searches utilizing Google...correct one!)    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  Contributing to Cellosaurus...

...a conformational change of the receptor which modulates the fluorescence intensity of the cpGFP without... vitro, and they further demonstrated that when tested in tobacco protoplasts, the enzymatic biosensor...

...with the article metadata, legend and user created notes on a “Pinterest for scientists”? ReFigure extension...profile. To see how to create ReFigures in a few minutes, visit our YouTube Channel. Currently we can only...

... four new plasmid pages that cover some of the hottest CRISPR tools. Base Edit: Base editing plasmids...Plan Your Experiment. #12DaysofCRISPR and blog updates We hope you enjoyed the #12DaysofCRISPR, a celebration...

...microbiology with multiple high-profile publications. My classmates consisted of graduate students, post-grads, and...manuscript is like generating a quilt with your lab mates, you add the individual figures and results to make...

...requested from Addgene many times as the technology disseminates throughout the research community. These popular...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...

...stayed to see the Opening Keynote address with Bill Gates. The long line for bag check was definitely worth...for global health and the various initiatives the Gates’ Foundation is involved with was one of the highlights...

... these meetings in relatively isolated resorts creates a sense of camaraderie with fellow attendees from...tackling this type of genetic engineering is a testament to how amazing the downstream payoff could be....

... was natural or synthetic 97.7% of the time in a test set of 173 known synthetic and natural genes.  ...saw that a percent sequence identity below 85% correlates with synthetic sequences. The researchers then...

... described by Li ZY, et al. PLoS One, 2016) and tested a series of mutations. Through this process mCarmine... mNeptune684, and has a favorable pKa of 5.6. To test out this new FP in your own experiments, mCarmine...

...fields of biology and enable the conversion of RNA templates to DNA but suffer from several important shortcomings...faithfully transcribes RNA to DNA with low error rates compared to other RTs. MarathonRT represents a major...