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Showing: 501 - 520 of 775 results

  1. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...transfers. The UBMTA was drafted by the National Institutes of Health (NIH) and adopted by the Association... Processing: When you place an order, Addgene generates the MTA and emails it straight to you, or we can...

  2. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...being plated on agar with ampicillin selection.  Notes and tips  We’ve found that our competent cells ...contamination.  Also conduct a positive control (test a plasmid that transforms well in other competent...

  3. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...Michael Koeris to learn how his company uses phage to test for Listeria in food products). I picked this post...Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific Enterprise in ...

  6. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...save you the time that would be spent making and testing entirely new gRNA designs. You can now easily find...variations (e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus to ensure...

  7. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...isolation of successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining...combining fluorescent tags, epitope tags, protease sites, and selection markers. These are especially useful...

  8. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...gives a presentation on what they do and how it relates to other teams at the company. This provides new...old) employees with information on how Addgene operates as a team and shows how they fit into the system...

  10. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...pyogenes system does not work well in Caulobacter). To test their CRISPRi system, the Laub lab repressed ctrA...that the levels of ctrA mRNA decreased after 20 minutes in xylose (which controls expression of dCas9)....

  12. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ... to its modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing it to be... customizing gene expression. Sahoo et al. have tested the system with genes up to 4 kb in length and ...

  13. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ..., or how organization may be altered in disease states. dCas9 gets a colorful makeover Even without its... cross-talk with the other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric...

  15. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...until now, you’ve always had to call the United States - Cambridge, MA to be exact. Today, that changes...expression of an unknown protein in macrophages/monocytes using lentiviral expression vectors. I also used...

  16. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...a conformational change of the receptor which modulates the fluorescence intensity of the cpGFP without... vitro, and they further demonstrated that when tested in tobacco protoplasts, the enzymatic biosensor...

  17. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...: Periodic surveying of cell line collections websites and catalogues Literature searches utilizing Google...correct one!)    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  Contributing to Cellosaurus...

  18. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...recommended for use in western blot, but we are actively testing it in other applications! If you try it out in ...

  20. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ... four new plasmid pages that cover some of the hottest CRISPR tools. Base Edit: Base editing plasmids...Plan Your Experiment. #12DaysofCRISPR and blog updates We hope you enjoyed the #12DaysofCRISPR, a celebration...
Showing: 501 - 520 of 775 results