...Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close proximity with the target protein, ...degraders provide distinct advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest...to co-opt the endogenous ubiquitin proteasome machinery to degrade any protein. We are particularly excited...FKBP12F36V-fusion and the ubiquitin proteasome machinery through binding with cereblon, leading to rapid...

...Kätzel D, Nissen W, Pekcec A (2019) Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide, clozapine...et al., 2019). Using PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules, were developed...be specifically and potently activated by pharmacologically selective effector molecules (PSEMs). Higher...

... efficiency and building prediction tools with machine learning (PRIDICT, PRIDICT2.0).   How to apply...chromatin-based prediction tool (ePRIDICT). In summary, machine learning-based prime editing prediction models ...computational biology. His expertise includes applying machine learning techniques to make sense of biology, with...B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction of prime editing efficiency...

...oncology, virology, immunology, microbiology, and pharmacology (many of which are represented in Addgene's ...cell culture over the last few decades some pharmacologists have tended to avoid cell culture in favour...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...

...typical PCR machines, the authors use affordable, field-ready, Bluetooth-enabled PCR machines that can even...approximately 1-2 hours Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer...mentioned, likely ~1-2 hours Equipment needed: PCR machines/Heat blocks, Lateral flow strips, Agarose gel ...

...greater than the one that can be achieved using pharmacological or electrophysiological methods (Deisseroth...depression-like behaviors: a critical analysis." Pharmacology Biochemistry and Behavior 122 (2014): 144-157...circuits in response to drug exposure." Neuropsychopharmacology 41.10 (2016): 2424. PubMed PMID: 26934955...applications of optogenetics." Current protocols in pharmacology 75.1 (2016): 11-19. PubMed PMID: 27960028. Van...

...phagocytosis (ADCP), Fcγ receptors on macrophages bind IgG antibodies. The macrophages then engulf and digest the...antibody-dependent cellular phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171...

...chromosomal DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally...lower temperatures and allows the replication machinery room to come in and get busy making copies.   ...incompatible because they will compete for the same machinery, creating an unstable and unpredictable environment...

...organelles, such as mitochondria, as well as macromolecules like actin and myosin, and enzymes for transmitter...two pathways also use different cytoskeletal machineries to facilitate transport: retrograde transport...interaction with uptake or intracellular transport machinery. Addgene offers viral preparations of AAV2-retro...serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex, Neurosci Res 93, 144-157. PubMed...

...Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab found that mismatch... developed a system in which the cellular MMR machinery is disrupted via a dominant negative mutation ...intended edit further weakened recognition by MMR machinery and improved editing efficiency. The lab also ...

...Bhang, a research investigator in the In Vivo Pharmacology group at Novartis Oncology, is an exciting new...her new role as an investigator in the In Vivo Pharmacology group at Novartis Oncology, Bhang has been working... Bhang is a research investigator in In Vivo Pharmacology at Novartis Oncology and is particularly interested...

... which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...compacted it becomes. This prevents the cellular machinery from physically interacting with the genes in ... transposon methylation underlies the mantled somaclonal variant of oil palm." Nature 525.7570 (2015):...

... it has been challenging to introduce CRISPRi machinery into diverse bacteria species. Existing protocols...bacteria with a chromosomal copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient...contain a chromosomal copy of the RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the...

...and departments will have qPCR machines available for use. ddPCR machines are not as commonly available.... PMID: 21554069. Haridan, U. S., Mokhtar, U., Machado, L. R., Abdul Aziz, A. T., Shueb, R. H., Zaid, ...

...doesn't require homology-directed repair (HDR) machinery, and could thus be used in non-dividing cells....convert adenine to inosine, which the translational machinery treats like guanine. This RNA editor would permit...as assessed using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral...

...This post was contributed by Rory Macneil, founder of Research Space. There are many types of electronic...equal. Many thanks to our guest blogger, Rory Macneil!  Rory is founder and CEO of Research Space, which...in Edinburgh. You can follow Rory on Twitter @rory_macneil.     Additional resources on the Addgene blog...

...temporal control than is achieved by traditional pharmacologic methods. A number of strategies have been developed...where to activate the pathway than traditional pharmacology techniques allow. Multiple photoswitches can...doi.org/10.1038/nn.2495 Marshel, J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja...

... Modification. Pharmaceutics, 13(5), 750. https://doi.org/10.3390/pharmaceutics13050750 Murlidharan, G...serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. Neuroscience Research, 93, 144...

...resulting in processing by the endogenous RNAi machinery. Alternatively, endogenous miRNA precursors can...initial state of the dsRNA upon activating the RNAi machinery (long dsRNA, typically hairpin RNA, or artificial...-2005-837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based ...

...2. Fluorescence Activated Cell Sorter (FACs) A machine that sorts cells according to whether or not they... deflect them into appropriate containers. The machine is useful for distinguishing cell populations that...PMCID: PMC2569002. 3. Egen, Jackson G., et al. "Macrophage and T cell dynamics during the development and...