...proteins (TniQ, Cas8, Cas7, Cas6) that forms the QCascade DNA-targeting module together with the crRNA.  ...requirement for multiple Cas proteins to form the Cascade targeting complex is a hallmark of Type I CRISPR-Cas...in regular Type I CRISPR-Cas systems, however, Cascade from VchINT does not recruit other nucleases (such... upstream 2-bp PAM sequence for recognition by QCascade; although 5’-CC-3’ is one of the best PAMs for...recognized by the system. Guided by the crRNA, QCascade complexes survey the genome and bind target DNA...downstream of the genomic target site bound by QCascade (Fig. 1B). The process also creates a 5-bp target-site...CRISPR-Cas systems by Tn7-like transposons. Proc Natl Acad Sci USA 114:E7358–E7366 . https://doi.org/10.1073...

...Whether you are pursuing an academic career path or moving in a nonacademic direction, there are many “...teamwork One of the defining characteristics of nonacademic science careers is that a team is almost always...others can be done in many ways… and many labs in academia benefit from strong collaborations to advance ...talk and own these accomplishments. Employers and academic departments want to be able to tell that you know...Development Framework Planner. For More Reading: From Academic Solos to Industrial Symphonies by Gwen Acton, ...

...career planning resources What Do I Do Now? Academic v. Non-Academic Career Decisions Explore alternative science...growth, and/or switch your field of work, both in academia and in industry. Advancing along a career path... advance without a PhD, but it’s possible.  In academia, the career ladder is smaller, but career growth... autonomy?  Before my master’s, I worked in an academic lab where I ran a handful of assays over and over...master’s. Career advancement for PhDs, both in academia and industry, often means moving from the lab ...

...the application process, and simplify access to academic resources, we created STEAMid, for a nationwide...common set of criteria such as citizenship status, academic year, and grades that serve as automated filters...opportunity to paint a comprehensive picture of your academic interests, potential, and drive. Read the associated...pull one another up while you are climbing the academic ladder. If you do not get accepted to your top...Program at Harvard. Roo wants to simplify access to academic resources among economically disadvantaged populations...resources that can help their children thrive academically. Rose is the co-founder of STEAMid.     Additional...

...of the academic sphere into a different meaningful career in science. Supervisors in the academic infrastructure...stated purpose of transitioning a scientist from academia to industry, you might want to consult the Cheeky...Cheeky Scientist. Their motto is “We Turn Academics into Confident and Successful Industry Professionals...scientist coaches can help with transitioning out of academia (because so many scientists are making this transition...Individual Development Plan Learn a Little about The Academic Labor Environment from the Future of Research ...

...currently in academia who are interested in such careers should take advantage of their academic environment...convening meetings of experts and leaders from academia, industry, community, education and government... After obtaining my PhD and working in digital academic publishing for a year, I’ve since returned to ...variety of other settings, including “conventional” academic venues such as scientific, educational or industry...in very technical and jargon-filled language in academic writing and presentations. But for many people...

... exciting literature. As I transitioned out of academia and away from the bench I certainly had concerns...needn't worry if they choose to move away from academic science; there is most likely abundant opportunity...field(s). Most anyone who has spent any time in academia has likely had the opportunity to attend and participate...including those based on Twitter. At my former academic department, we had two recurring journal clubs...both the number of participants and the standard academic calendar. So you want to start a journal club?... the meeting. This is a common failing in many academic journal clubs where institutional inertia drives...

...the Addgene Blog Read What Do I Do Now? Academic v. Non-Academic Career Decisions Learn about Science Career...the life sciences prepares one for numerous non-academic careers. Still, many start their scientific careers...careers hoping to follow the traditional academic route (find tips for getting a faculty position here...to start pursuing other options. While some non-academic career options require additional study for late.... These roles offer an easy transition from an academic lab to industry without being too commercial or...

...professor in synthetic biology from the Chinese Academy of Sciences. In the Yang lab, we’re continually... system contains 4 plasmids: pDonor, pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable cargo...expresses the transposases TnsA, TnsB, and TnsC pQCascade expresses the transposase TniQ, the Cas proteins...needs 2 newly constructed plasmids (pDonor and pQCascade) with 4 rounds of transformation to complete the...bloggers! Qi Li obtained her Ph.D. from the Chinese Academy of Sciences (CAS) Center for Excellence in Molecular...from Shanghai Institute of Biochemistry, Chinese Academy of Sciences. He joined Shanghai Institutes for ...

...years worth!) with academic scientists who can’t help us learn about the nonacademic sphere where most ...Training as a scientist in the academic system has many pluses. I delighted in my graduate school years...on the science I love. This immersive nature of academia often means that scientists-in-training rarely...opportunity to learn about the myriad of diverse, nonacademic careers that will be available once they have...

... path from academia is the uncertainty of it all. Though I had spent the first few decades of my life ...university policy to combat sexual harassment in academia or a large company who decides how they are going...researchers who have decided to transition out of academia from graduate school or a postdoc. But some are...either a sabbatical year or transition away from academia as tenured professors. There’s never a bad time...positions, but will also make your transition from academia to the policy world much smoother.  Action Items...

...Actually, it is crucial for all scientists, academic and non-academic, to always be meeting as many other scientists...profile in the community Job hunting outside of academia is all driven by personal connections What can...met on LinkedIn, especially if they are not in academia. Make sure to learn how to use LinkedIn properly...congratulations on job changes and promotions.  Follow academic scientists on ResearchGate and/or via Pubmed alerts...

...distinguish highly similar proteins. For example, N-cadherin (cadherin-2) is a postsynaptic cell adhesion protein... By fusing N-cadherin to smFPs the Looger team was able to specifically label N-cadherin in neurons and...map these neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV) has been...

...Hopefully you know that, if you’re an academic researcher at a nonprofit institution, you can order plasmids...pyranopyridine derivatives." Proceedings of the National Academy of Sciences 113.13 (2016): 3509-3514. Tikhonova...metabolite production." Proceedings of the National Academy of Sciences 113.9 (2016): 2388-2393. Huang, Shijie...antibiotic resistance." Proceedings of the National Academy of Sciences 109.52 (2012): 21408-21413. PUF Assembly... family scaffold." Proceedings of the National Academy of Sciences 108.38 (2011): 15870-15875. See Kendall...

...function through multiple intra-cellular signaling cascades. Optical methods for controlling GPCR activation...control GPCR activation and downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including ...extracellular domain which activates the native signaling cascade of the intracellular part of the receptor, similar... out important branch points along a signaling cascade that may go awry in a disease state.      ... membrane channel. Proceedings of the National Academy of Sciences, 100(24), 13940–13945. https://doi....

...proteins (TniQ, Cas8, Cas7, Cas6) that forms the QCascade DNA-targeting module with the crRNA, 3) a transposase...For the INTEGRATE system to transpose, first the QCascade complex surveys the genome and binds its target...molecule, and inserts it ~50 bp downstream of QCascade’s genomic target site (Fig. 3B). The INTEGRATE ...of RNA-guided transposition with INTEGRATE. The QCascade complex surveys the genome and binds its target...molecule, and inserts it ~50 bp downstream of QCascade’s genomic target site.  T-RL= transposon right-...

...positive perspective on the transition to a post-academic career. This training was “included” with their...training for scientists destined to continue in academia. It seems it’s largely a matter of luck if a student...together with the goal of driving systemic change in academic career readiness training. It's been an honor ...all science training programs. I believe in the academic apprenticeship system as a training ground for...

...were they all to pursue jobs in academia. Certainly funding for academic research and training is getting...get jobs. They are not exposed to the vast non-academia career landscape and there is insufficient (or...Initiative (PCDI) in the Netherlands. There are many academic administrators who are finding ways to provide...

...scientists.  At the same time, he pointed out, academic research has some big problems resulting from ...statistic that only 15% of current postdocs will be academic principal investigators. She also pointed out ...that the PhD and Postdoc advisors only know about academia and alternate training needs to be provided to..., gave examples of how you don’t have to be in academia to make change.  He talked about his own unique...

...study published in Proceedings of the National Academy of Sciences in March, he and his colleagues took...time? Howarth: The Proceedings of the National Academy of Sciences paper [published online on March 17...magnetic cancer cell capture. Fierer et al (Proc Natl Acad Sci U S A. 2014 Mar 17. PubMed) Peptide tag forming...engineering a bacterial adhesin. Zakeri et al (Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012...