...CRISPR-Cas and Cascade for large genomic deletions.... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3 Browse CRISPR...only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR- as sociated c omplex for a ntiviral ...combination of Cas5, Cas6, Cas7, Cas8, and Cas11. Cascade is directed to target DNA using a gRNA and recruits... difficulties in getting all components of the Cascade complex to express equally. To combat this, some...take an all-in-one approach and include Cas3 and Cascade on one single plasmid, though many still separate...individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse...

...career outside the traditional academic silo to tenured professor.  Non-academia encompasses millions of choices...designate non-academic jobs as “alternative” anymore.   Since most scientists start out in academia, it is...and perspectives to consider. What do academic and non-academic careers in science have in common? You...but there is more flexibility with the academic calendar Academic researchers must be extreme self-starters...Biomedical workforce will NOT go into “traditional” academic positions. More recently, I have been hearing ...careers as their training progresses.  In From Academic Solos to Industrial Symphonies, an article well...jump into industry.” In his article On Leaving Academe, Terran Lane is asked by his colleagues and friends...

... industry and academia. Pros and cons: The academic vs. industry postdoc  Time: Academic postdocs can ...still incredibly nervous about leaving academia. A lot of academics tend to view industry as “the dark side...your goal is to enter academia, it is easier to make that transition from an academic setting—especially ..., an academic advisor could also lose their funding. Communication: In both industry and academia, you...AstraZeneca’s Waltham site after doing a brief academic postdoc. Why did I switch? Well, my long-term ...acquire. Learning about drug discovery: Unless your academic lab is closely involved with clinical trials or... be a bit of a mystery to graduate students in academia. Getting a drug to the market usually takes over...

...compositions of the Cascade complex. Created with BioRender.com.   The Cascade complex Cascade is an important...capabilities of Cas3 and its partner in crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A...multi-Cas complex called Cascade (CRISPR-associated complex for antiviral defense). Cascade is brought to the...Cas11. Type I-E Cascades contains all five of these Cas proteins, while type I-C Cascades do not contain...transposase systems utilize with type I-F Cascades. Figure 2: Cascade complex from CRISPR type I-E. Created...components of Cascade for maximum efficiency of the process. Various labs have designed all-in-one Cascade plasmids...species and will have different compositions of Cascade. Although seemingly ubiquitous in nature, they ...

....   Advice for success in academia How can we shape our time in academia to maximize productivity and...who's seen every spurious result under the sun. Academia is about convincing results, and nothing is more...pragmatic.” Advice for pursuing careers outside of academia Recently, graduate students and postdocs have...have become more aware of careers outside of academia. How do PIs assist their mentees who have goals outside...recommends that a student interested in leaving academia should present their research at conferences to... applicable to a variety of careers outside of academia. She has had previous graduate students and postdocs... success in academia...

... When transitioning from an academic science career path to a non-academic one, one of the biggest changes...Curricula Vitae to showcase your publications, academic performances, and research experiences, you now...consider when you're preparing your resume for a non-academic position: What is a resume? How is it different.... If you are still debating whether to stay in academia or not, it is even more important to start early... about your plan for the next 5 years. Does an academic career work well with your life and career plan...emphasis for graduate student resumes UC Irvine Non Academic Job Search Handbook & Resumes  Read Our Blog Post...

... Are you currently on or planning to go on the academic job market? In addition to all of the documents...

... became available: distribution was limited to academic scientists. Expanding plasmid sharing during ...plasmids available more broadly: to scientists in academia and industry. As a result over 90% of Addgene’...                                       Chinese Academy of Medical Sciences University of Texas at Austin...these plasmids to the scientific community - both academic and industry scientists. This partnership complemented...organizations have made their plasmids available beyond academia. For example: Plasmids from the Michael J. Fox...scientists To make plasmid sharing more open for academic and industry scientists, we’ve worked with many...

...outside of academia. These roles can include positions, like mine, that help support academia but the skills... did I join Addgene? Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon...dramatic story, but it wouldn’t be my story. I love academics. If I didn’t, it would have been unwise of me ...pretend that that there aren’t problems with the academic enterprise or that there weren’t some pretty hefty...are led to believe that they can all end up in academic positions (I’ve even blogged about this here and... my story demonstrates that transitioning from academia doesn’t have to mean abandoning it or running ...more glad that I thought about careers outside of academia during my graduate training. PhDs have many roles...

...Manufacturers rarely provide the construction plans or CAD (computer aided design) drawings of their devices...

... and advice for grauated students pursuing non-academic careers....

...by measuring the titer of samples obtained from academic viral vector cores. Our titers were similar to...

...enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is ...separate Cas enzymes that form a complex called Cascade (see Cas3 for more details), while type V-K systems... by Cas9 systems. Cas3 must be paired with the Cascade ( C RISPR- as sociated c omplex for a ntiviral ...de fense) complex to initiate DNA degradation. Cascade is made of five proteins: Cas8 (Cse1), Cas11 (Cse2...Overview of editing with Cas3 Browse Plasmids: Cascade-Cas3 Want more information on the wide variety ...nucleases like Cas9 and Cas12a (also known as Cpf1) Cascade C RISPR- as sociated c omplex for a ntiviral de...Lewis, J. D., & Bondy-Denomy, J. (2020). A compact Cascade–Cas3 system for targeted genome engineering. Nature...

...potently activated by an inert ligand. Proc Natl Acad Sci USA. 104:5163–68. PMID: 17360345 (Link opens...biological light sources for opsin activation. Proc Natl Acad Sci USA. 113:E358-67. PMID: 26733686 (Link opens...specifically designed Gi-coupled receptor. Proc Natl Acad Sci USA. 95:352–57. PMID: 9419379 (Link opens in...regulation of β cell function in vivo. Proc Natl Acad Sci USA. 106:19197–202. PMID: 19858481 (Link opens...

...Gamma-protocadherin-A3 [N144/5R] Gamma-protocadherin-A3 Mouse Mouse IgG2a 222155 Gamma-protocadherin-B2 ...Mouse IgG2a 164156 Anti-Gamma-protocadherin-C3 [N174B/27R] Gamma-protocadherin-C3 Mouse Mouse IgG2a 164157... 177503 Anti-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a 177504...IgG2a 182105 Anti-Pan-Gamma-protocadherin-A [N144/32R] Pan-Gamma-protocadherin-A Mouse IgG2a 182106 Anti-Histone...Mouse IgG2a 190299 Anti-Gamma-protocadherin-B2 [N148/30] Gamma-protocadherin-B2 Mouse Mouse IgG2a 190300...Mouse IgG2a 199398 Anti-Gamma-protocadherin-A3 [N144/17R] Gamma-protocadherin-A3 Rat Mouse IgG2a 199399 Anti-PINK1...Pan-Gamma-protocadherin-Constant recombinant mouse monoclonal antibody. Pan-Gamma-protocadherin-Constant...

...enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is ...separate Cas enzymes that form a complex called Cascade (see Cas3 for more details), while type V-K systems... by Cas9 systems. Cas3 must be paired with the Cascade ( C RISPR- as sociated c omplex for a ntiviral ...de fense) complex to initiate DNA degradation. Cascade is made of five proteins: Cas8 (Cse1), Cas11 (Cse2...Overview of editing with Cas3 Browse Plasmids: Cascade-Cas3 Want more information on the wide variety ...nucleases like Cas9 and Cas12a (also known as Cpf1) Cascade C RISPR- as sociated c omplex for a ntiviral de...Lewis, J. D., & Bondy-Denomy, J. (2020). A compact Cascade–Cas3 system for targeted genome engineering. Nature...

...mouse E-cadherin GFP (423) Adherens Junctions E-cadherin EGFP Alpha Yap 71366 Murine E-cadherin mCherry...sfGFP David Spray 54959 mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson 55165 ...mCherry Adherens Junctions E-cadherin mCherry Alpha Yap 54219 mEmerald-Paxillin-22 Focal Adhesions paxillin...

...transgene-free induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi...cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. Jaenisch Lentivirus...modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek Fibroblasts Cardiomyocytes...human fibroblasts to dopaminergic neurons. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8. Parmar Fibroblasts...self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32. Wernig Fibroblasts...

... of Cre or to recombine into BAC SP6 Bacterial Mercader Huber 108543 pLV-EF1-Cre-PGK-Puro Cre EF-1 alpha...Cre-ERT2; endothelial reporter fli1a Zebrafish Mercader Huber 108995 pDM1488 Cre; NeoR act15 D. discoideum...Site-Specific Recombination in Transgenic Mice. Proc Natl Acad Sci. Jul 15;89(14):6232-6. PubMed . Matsuda T, Cepko...Introduced by In Vivo Electroporation. Proc Natl Acad Sci. Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104...the Cre Recombinase of Bacteriophage P1. Proc Natl Acad Sci. Jul;85(14):5166-70. PubMed . Sternberg N, Hamilton...Conditional RNA Interference from Transgenes. Proc Natl Acad Sci. Jul 13;101(28):10380-5. doi:10.1073/pnas.0403954101...

...genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Ca2+ indicators...Zn2+ with genetically encoded sensors. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Amy Palmer...revealed by single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008 Jan 8. Wolfgang Dostmann cGMP (cyclic...release at Schaffer collateral synapses. Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. Katalin ...and neurons of Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2019 Aug 1. Alexander Gottschalk Voltage...