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  1. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...CRISPR-Cas and Cascade for large genomic deletions.... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3 Browse CRISPR...only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR- as sociated c omplex for a ntiviral ...combination of Cas5, Cas6, Cas7, Cas8, and Cas11. Cascade is directed to target DNA using a gRNA and recruits... difficulties in getting all components of the Cascade complex to express equally. To combat this, some...take an all-in-one approach and include Cas3 and Cascade on one single plasmid, though many still separate...individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse...

  2. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Gamma-protocadherin-A3 [N144/5R] Gamma-protocadherin-A3 Mouse Mouse IgG2a 222155 Gamma-protocadherin-B2 ...Mouse IgG2a 164156 Anti-Gamma-protocadherin-C3 [N174B/27R] Gamma-protocadherin-C3 Mouse Mouse IgG2a 164157... 177503 Anti-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a 177504...IgG2a 182105 Anti-Pan-Gamma-protocadherin-A [N144/32R] Pan-Gamma-protocadherin-A Mouse IgG2a 182106 Anti-Histone...Mouse IgG2a 190299 Anti-Gamma-protocadherin-B2 [N148/30] Gamma-protocadherin-B2 Mouse Mouse IgG2a 190300...Mouse IgG2a 199398 Anti-Gamma-protocadherin-A3 [N144/17R] Gamma-protocadherin-A3 Rat Mouse IgG2a 199399 Anti-PINK1...Pan-Gamma-protocadherin-Constant recombinant mouse monoclonal antibody. Pan-Gamma-protocadherin-Constant...

  3. CRISPR Guide

    Type
    Collection
    ...enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is ...separate Cas enzymes that form a complex called Cascade (see Cas3 for more details), while type V-K systems... by Cas9 systems. Cas3 must be paired with the Cascade ( C RISPR- as sociated c omplex for a ntiviral ...de fense) complex to initiate DNA degradation. Cascade is made of five proteins: Cas8 (Cse1), Cas11 (Cse2...Overview of editing with Cas3 Browse Plasmids: Cascade-Cas3 Want more information on the wide variety ...nucleases like Cas9 and Cas12a (also known as Cpf1) Cascade C RISPR- as sociated c omplex for a ntiviral de...Lewis, J. D., & Bondy-Denomy, J. (2020). A compact Cascade–Cas3 system for targeted genome engineering. Nature...

  4. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mouse E-cadherin GFP (423) Adherens Junctions E-cadherin EGFP Alpha Yap 71366 Murine E-cadherin mCherry...sfGFP David Spray 54959 mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson 55165 ...mCherry Adherens Junctions E-cadherin mCherry Alpha Yap 54219 mEmerald-Paxillin-22 Focal Adhesions paxillin...

  5. Plasmids for Stem Cell Research

    Type
    Collection
    ...transgene-free induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi...cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. Jaenisch Lentivirus...modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek Fibroblasts Cardiomyocytes...human fibroblasts to dopaminergic neurons. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8. Parmar Fibroblasts...self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32. Wernig Fibroblasts...

  6. Cre-lox system

    Type
    Collection
    ... of Cre or to recombine into BAC SP6 Bacterial Mercader Huber 108543 pLV-EF1-Cre-PGK-Puro Cre EF-1 alpha...Cre-ERT2; endothelial reporter fli1a Zebrafish Mercader Huber 108995 pDM1488 Cre; NeoR act15 D. discoideum...Site-Specific Recombination in Transgenic Mice. Proc Natl Acad Sci. Jul 15;89(14):6232-6. PubMed . Matsuda T, Cepko...Introduced by In Vivo Electroporation. Proc Natl Acad Sci. Jan 16;104(3):1027-32. doi: 10.1073/pnas.0610155104...the Cre Recombinase of Bacteriophage P1. Proc Natl Acad Sci. Jul;85(14):5166-70. PubMed . Sternberg N, Hamilton...Conditional RNA Interference from Transgenes. Proc Natl Acad Sci. Jul 13;101(28):10380-5. doi:10.1073/pnas.0403954101...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Ca2+ indicators...Zn2+ with genetically encoded sensors. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Amy Palmer...revealed by single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008 Jan 8. Wolfgang Dostmann cGMP (cyclic...release at Schaffer collateral synapses. Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. Katalin ...and neurons of Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2019 Aug 1. Alexander Gottschalk Voltage...

  8. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...zebrafish as described by Moore et al. PLoS One 2012 and Cade et al., Nucleic Acids Res 2012 ) pCLR2070 (for expression...zebrafish as described by Moore et al. PLoS One 2012 and Cade et al., Nucleic Acids Res 2012 )...

  9. Brain Initiative Collection

    Type
    Collection
    ...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV9 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1 pAAV-EF1a-iCreV can...

  10. Resolute Plasmid Collection

    Type
    Collection
    ...public-private partnership of 13 members from industry and academia financed by the Innovative Medicine Initiative...the field of SLCs to the global benefit of basic academic research as well as applied research in small ...

  11. Zinc Finger Consortium Reagents

    Type
    Collection
    ...expressing zinc finger proteins for distribution to academic and nonprofit laboratories for research and educational...

  12. NETRF

    Type
    Collection
    ...published materials and resources available to academic researchers around the world. The NETRF is grateful...

  15. CRISPR Plasmids - Parasites

    Type
    Collection
    ...have been the focus of biomedical research for decades. Recent developments with CRISPR-Cas9 gene editing...

  16. CRISPR Plasmids and Resources

    Type
    Collection
    ...DNA without promoting DSB formation. Delete NEW Cascade-Cas3 generates large genomic deletions. Insert ...

  19. TALEN Guide

    Type
    Collection
    ...zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh...

  20. Ras Pathway

    Type
    Collection
    ...and differentiation. Schematic of Ras signaling cascade that starts at the plasma membrane. Cancer Impact...
Showing: 1 - 20 of 27 results