...), the REAL-Fast assembly method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based...REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast and FLASH...designed TALENs, and assembly methods for REAL, REAL-Fast and FLASH Genome...2013 ) More detailed information about REAL, REAL-Fast and FLASH, including detailed and up-to-date protocols...construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This new version of ZiFiT Targeter...target that guide the user through the REAL or REAL-Fast assembly process or lists of plasmid ID numbers ...

...effector repeat arrays assembled using the REAL, REAL-Fast or FLASH methods... Vectors TALEN Expression Vectors for REAL, REAL-Fast and FLASH You may also like... Keith Joung Lab plasmids...effector repeat arrays (assembled using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as... TALENs. Additional details about the REAL, REAL-Fast, and FLASH assembly methods can be found on the ...

... pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning into a wide range of Gateway-compatible...

...jGCaMP8 Fast Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically...sensors (fRCaMP1/2, GECO1/2) The kinetic mechanisms of fast-decay red-fluorescent genetically encoded calcium...detection (iAchSnFR) (Constitutive or Cre-dependent) A fast genetically encoded fluorescent sensor for faithful... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell... to detect neural activity (FlicR1) A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...Yulong Li Dopamine Ultrafast neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging...

...Monomer pFast-FT-N1 - Mammalian Expression pTRE-Fast-FT - Mammalian Expression pBAD/HisB-Fast-FT - Bacterial...nm) Brightness pKa Maturation Structure Plasmids Fast-FT 403(blue), 583(red) 466(blue), 606(red) 14.9(...

... Restriction Cloning and Fast Cloning Requirement Restriction Cloning Fast Cloning Choose Restriction...of the colonies are correct. Good luck with this fast and easy technique! Many thanks to our guest blogger... Transformation + + The FastCloning process in brief In FastCloning, you PCR both the vector and ...and ligated in vivo. Detailed FastCloning procedure To start the FastCloning process, the insert and vector...most importantly, excessive time requirements. FastCloning is a simpler yet reliable cloning technique that...understanding of the step by step procedure of FastCloning, here are some tips for high efficiency cloning...PubMed PMID: 27391134. 2. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...

...check out our blog post on choosing a Cas9 enzyme. Fast facts – PAM requirement: NGG and an expanding number...has anti-viral application potential as a result. Fast facts – PAM requirement: AAG or TTC  Best for: very...optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or TTTN. Best for:...variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best for: high fidelity...information on Cas13, check out our blog on RNA editing. Fast facts – PAM requirement: protospacer flanking sequence...11 in our blog post on its development and use. Fast facts – PAM requirement: protospacer flanking sequence...targeting single stranded RNA.   Table 1: Fast facts on Cas family proteins and genome editing ...

...effects can mask weaker ones. Thus, the need for a faster and higher-throughput in vitro approach. Using ...

...high levels of correct assemblies) translate to faster assembly workflows that require less screening ...

...selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko...

...that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. 4. Stem...after the OSKM paper – as a prime example of the fast movement in this field.  As an Addgene scientist...engineering technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster...

...to the grant officers at Fast Grants. We received a miraculous grant from Fast Grants, which was approved...coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority of deposited materials...

...Addgene does a lot to ensure that we can facilitate a fast and agile MTA process. This is important to keep... last twenty years! This has helped us develop a fast, agile approach, which we’ve updated and refined...organization upon purchase. We recognize exceptionally fast tech transfer offices with the Golden Stopwatch ...

... 6.8 (red) 2.8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579...produced the three separate timers shown in the table: Fast-FT, Medium-FT, and Slow-FT. These display progressively...Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red) ...

...Self-labeling tags for single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors... https://doi.org/10.1101/2024.12.20.629623.  A fast and responsive voltage indicator with enhanced sensitivity...can be used in different illumination regimes with fast activation kinetics and high responsivity near the...Z., Jiang, D., Zhang, G., … Lin, M. Z. (2024). A fast and responsive voltage indicator with enhanced sensitivity...Lab and collaborators recently created ASAP5, a faster and more responsive genetically-encoded voltage...

... of CRISPR/Cas9 technology are coming along at a fast and furious pace. In December, as we've blogged ...it before, but CRISPRs allow research to move so fast." On the very same day the monkey story hit the ...

...your preference, we are here to make it fast and easy. Fast Approval: The UBMTA used by Addgene is ...approvals, so your plasmid requests can get to your lab faster. For Everybody: Friendly Support: Have a question...

...BACs, what can I do? Chemically competent cells are fast and easy to use, but are less efficient at taking...

...effector repeat arrays (assembled using the REAL, REAL-Fast, or FLASH methods) for expression as TALE-LSD1 fusions...

...Kinetics How fast is the process you would like to monitor? Neurons, for example, have unusually fast calcium...three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but slower)....