... Restriction Cloning and Fast Cloning Requirement Restriction Cloning Fast Cloning Choose Restriction...of the colonies are correct. Good luck with this fast and easy technique! Many thanks to our guest blogger... Transformation + + The FastCloning process in brief In FastCloning, you PCR both the vector and ...and ligated in vivo. Detailed FastCloning procedure To start the FastCloning process, the insert and vector...most importantly, excessive time requirements. FastCloning is a simpler yet reliable cloning technique that...understanding of the step by step procedure of FastCloning, here are some tips for high efficiency cloning...PubMed PMID: 27391134. 2. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...

...check out our blog post on choosing a Cas9 enzyme. Fast facts – PAM requirement: NGG and an expanding number...has anti-viral application potential as a result. Fast facts – PAM requirement: AAG or TTC  Best for: very...optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or TTTN. Best for:...variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best for: high fidelity...information on Cas13, check out our blog on RNA editing. Fast facts – PAM requirement: protospacer flanking sequence...11 in our blog post on its development and use. Fast facts – PAM requirement: protospacer flanking sequence...targeting single stranded RNA.   Table 1: Fast facts on Cas family proteins and genome editing ...

...effects can mask weaker ones. Thus, the need for a faster and higher-throughput in vitro approach. Using ...

...high levels of correct assemblies) translate to faster assembly workflows that require less screening ...

...that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. 4. Stem...after the OSKM paper – as a prime example of the fast movement in this field.  As an Addgene scientist...engineering technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster...

...to the grant officers at Fast Grants. We received a miraculous grant from Fast Grants, which was approved...coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority of deposited materials...

...Addgene does a lot to ensure that we can facilitate a fast and agile MTA process. This is important to keep... last twenty years! This has helped us develop a fast, agile approach, which we’ve updated and refined...organization upon purchase. We recognize exceptionally fast tech transfer offices with the Golden Stopwatch ...

... 6.8 (red) 2.8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579...produced the three separate timers shown in the table: Fast-FT, Medium-FT, and Slow-FT. These display progressively...Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red) ...

...Self-labeling tags for single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors... https://doi.org/10.1101/2024.12.20.629623.  A fast and responsive voltage indicator with enhanced sensitivity...can be used in different illumination regimes with fast activation kinetics and high responsivity near the...Z., Jiang, D., Zhang, G., … Lin, M. Z. (2024). A fast and responsive voltage indicator with enhanced sensitivity...Lab and collaborators recently created ASAP5, a faster and more responsive genetically-encoded voltage...

... of CRISPR/Cas9 technology are coming along at a fast and furious pace. In December, as we've blogged ...it before, but CRISPRs allow research to move so fast." On the very same day the monkey story hit the ...

...your preference, we are here to make it fast and easy. Fast Approval: The UBMTA used by Addgene is ...approvals, so your plasmid requests can get to your lab faster. For Everybody: Friendly Support: Have a question...

...Kinetics How fast is the process you would like to monitor? Neurons, for example, have unusually fast calcium...three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but slower)....

...Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol...

...Verkhusha Multiple FAST Fluorescence-activating and absorption shifting tag (FAST) for use with green-yellow...superpositive GFP Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting protein-protein...mScarlet Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of endogenous proteins...requires some time for chromophore maturation, splitFAST displays rapid and reversible complementation....

...blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field, requiring people who are willing to...some of the more challenging parts of the job? With fast turnover of projects, pressure to maintain consistently...case, consulting can sometimes be challenging. The fast paced nature of projects often means working long...

... the ability to do DNA synthesis, is evolving so fast that it is a really nice opportunity to study it...happens when technological change happens really fast,” Thompson says. To do this, Thompson reached out...

...imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...

...characteristic to capture in these types of samples is their fast movements. Depending on the sample, a temporal resolution...sample is then collected and projected back to a fast, sensitive detector such as a scientific CMOS camera...planes of a sample may also be useful for providing fast imaging along with optical sectioning (5). One such...preferred technique for many of these samples due to its fast temporal resolution and decreased phototoxicity....captured in a single camera exposure. This is much faster than relying on raster scanning as is done in confocal...

... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...transfer at the subcellular level requires a probe with fast kinetics and high signal/noise ratio. Here, Takeharu...Figure 7: B-gTEMP temperature-sensitive probe enables fast measurement of temperature and local heat transfer...affinity for glutamate but slower kinetics; v857 has faster kinetics but lower affinity) and with a variety...two-photon microscopy. The resulting JEDI-2P has faster response kinetics and increased brightness, sensitivity...

...easy to manipulate, it’s affordable, and it grows fast to boot. The yeast strains S. cerevisiae and S. ...to culture and easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic...