We narrowed to 236 results for: Xpa
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TypeBlog Post...expression (Wang et al., 2016). In 2018, the lab expanded on this tool by splitting cGAL4 in two and binding...
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Hot Plasmids - October 2020
TypeBlog Post...structure and function, with the advent of each tool expanding our ability to study and understand the complex... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post... innovative methods greatly improved CRISPRa, expanding its practicality and popularity in research (Tanenbaum... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...-mount IHC techniques on rodent brains and is expanding to more complex brain systems such as non-human... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...a suitable PAM cannot be edited efficiently. To expand the targeting scope of ABE8, the team replaced ... -
CRISPR in the Clinic
TypeBlog Post... functional correction of the gene in clonally expanded organoids. This work, and many others, pushed ... -
Engaging with science and society at pgEd
TypeBlog Post... This “general public” audience comprises the taxpayers who fund much of scientific research and who will... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...expression by delivering shRNA or miRNA. The ever expanding CRISPR field makes wide use of retroviral delivery... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...(Sotos-type 2 and Marshall-Smith) and could be expanded to NFIA-, NFIB- and NFIC-related human syndromes... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...encourage you to deposit them back with Addgene and expand the number of genes that other classrooms and researchers... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...here. Listen to the Hot Plasmids episode! Expanding the optogenetics toolbox with CRY2clust Article... -
Plasmids 101: Modular Cloning
TypeBlog Post...Scripps Research, where he works on genetic code expansion. References and Resources References Weber E... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...international travel, the Zika virus is expected to expand into heavily populated regions of South, Central... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...PMID: 12050007. 7. Stockschläder, M., et al. "Expansion and fibronectin-enhanced retroviral transduction... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...on expression of a surface marker, subcloning, expansion, and confirmation. This procedure can take several... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...developments in this field. Nobel Laureates and the ever expanding importance of RNA The second keynote lecture was... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...experiments. Mobile-CRISPRi applications Mobile-CRISPRi expands CRISPRi to non-model bacteria including pathogenic... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...takes 4-7 days, not counting the time needed to expand the HEK293 cells. Check out the Addgene AAV Production... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...on Cas9 or a derivative of Cas9, but the ever-expanding repertoire of CRISPR has brought many other Cas... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...off-target editing rates. This PAM flexibility should expand applications that require very specific targeting...