...effects xCas9 3.7 - mutations in multiple domains to expand PAM recognition, increase specificity, and decrease...recognizes NNTA, a PAM sequence often found in plants, expanding the targeting range for plant genome engineering...cleave ssDNA non-specifically. Cas12a also has an expanded targeting range to AT-rich regions, as it recognizes.... (2018). Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science , 361 (6408), 1259–1262...

...lentiviral genome and those cells that survive can be expanded to create stable cell lines, which have incorporated...use FACS to sort cells expressing GFP and later expand these cells into a cell line. Where does lentivirus...

...CRISPR Chemogenetics Optogenetics Genetic Code Expansion...

.... Gene Ther. 2001 Aug;8(16):1248-54. PubMed . Expanding AAV packaging capacity with trans-splicing or ...

...ChR Variants. The optogenetic toolbox has been expanded by scientists through both the identification ...

... target cells at a high efficiency. Addgene's expanding viral service offers select ready-made AAV and...