...  Second, expansion introduces distortions. The goal in expansion is for your sample to expand evenly in...equipment needed for EM or SRM? Enter Expansion Microscopy. What is Expansion Microscopy?  ExM provides a straightforward...the protocol, samples can be expanded to ~4-10x linearly! This expansion physically separates molecules...allow for even expansion.  In some cases, labels are added after this stage.  Step 4: Expand! (Typically...are typically easier to expand than mouse brain slices, which are easier to expand than, say, a C. elegans...and after expansion and measure the degree of agreement between them to ensure the expanded images are...lines in pre-expanded samples can sometimes take on a fragmented appearance post-expansion, but that doesn...

...permissions and paperwork. Through updating and expanding our website, we’ve learned about the importance...

...We’re very excited to announce that we are expanding our viral vector service to include a Packaged on... on Request option. Through this expansion, scientists will be able to request an AAV composed of an AAV... vector prep.    Excited? So are we! This expansion will give researchers increased flexibility when...when planning their research and expanded choice when it comes to ordering high-quality materials from... overview in this blog post.   When will the expansion happen?  You will be able to request Packaged ... their material page. Interested scientists can expand the banner to select their serotype of choice, ...resources.  We hope you’re as excited about this expansion as we are! Happy packaging! ...

...may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical amino...introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and techniques...number of experiments with. The catch The rapid expansion of GCE led to the creation of many new tools and...Tan, Z., & Cornish, V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry...

...REC3, and PAM interacting domains and allows for expanded PAM recognition as well as increased specificity...synthetic gRNA) are derived from the same species. Expanding the CRISPR toolbox The isolation of novel CRISPR...

...et al., 2016) and since then has been trying to expand their precision editing capabilities. Base editors...with many Cas domains beyond SpCas9. In order to expand the gene-editing applications and targeting scope...

...scientific sharing The EDGE Program is designed to expand the number of systems that can be studied by providing...

...connections. Expanding the viral vector knowledge base: You can help! We’ve been expanding our viral vector...ready-to-use viral preps and we’ll be continually expanding our collection. We’ll provide a brief rundown ...find our updated viral vector pages as well as expanded virus protocols, information on how we produce...email to [email protected]. Your questions help us expand and improve our resources for future users. We’...

...merely 20. Expanding RNA Editing Capabilities through RESCUE Two years later, the Zhang lab expanded the RNA...With the expanded targeting ability using both systems, the authors look forward to the expanded use of ...precise RNA editing. The addition of RESCUE further expands the RNA editing toolkit and sequences that can ...

...different countries, into our repository, while expanding global access to our repository through our distributor...partners, with the launch of antibodies in China and expansion of viral services available in South Korea.  ... Viral Vectors 101 eBook. We’ll also focus on expanding international access to the Addgene repository..., Korea, Japan, India, and Brazil. Through an expansion of our export license application service, we’...

...distribution was limited to academic scientists. Expanding plasmid sharing during COVID-19 Realizing this...to industry scientists  While we’ve focused on expanding COVID-19 plasmids to industry scientists in the...that support us in our mission.  While rapidly expanding our COVID-19 resources has been our focus for ...transfer office to discuss the possibilities for expanded sharing. Is there a plasmid you're hoping will...

... lab cooler boxes Some vendors take back their expanded polystyrene (a.k.a. Styrofoam or cooler boxes)...when receiving temperature-controlled reagents. Expanded polystyrene will likely end up in a landfill, ...associated with CO2 emissions. NEB takes back expanded polystyrene boxes for free (in the U.S.). Their... program in 1976! Millipore Sigma takes back expanded polystyrene boxes for free (in the U.S.). After...

...they meet other Mentors and Singletons, and vastly expand their networks as they naturally find people with...briannadoesbio) June 22, 2019 Favorable conditions - Expansion of networks The last stage is Exponential Growth...mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons to other scientific conferences...such mentoring programs, scientists can build and expand their networks and further their career development...

...Seeding Labs, a nonprofit founded in 2007, strives to expand the opportunities and scientific resources available...first step; going forward, Seeding Labs hopes to expand its activities by facilitating connections to mentorship...regions like South America and Africa, but we need to expand our efforts to find new and creative ways to reach...

...physiology, development, and disease. In order to expand their model’s impact, organoid users have recently...months: Our selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9... INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON...

...for light mediated neuronal control and we are expanding our viral service to make these readily available... experiment. The Addgene viral service has now expanded to offer many of our control plasmids in new serotypes... is vital in studying neural networks. We have expanded our calcium sensor collection to include our first...

...Our previous blog post The PAM Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity...toolkit available to researchers will continue to expand. Final thoughts Since the first use of Cas9 to ..., et al. "Engineered CRISPR-Cas9 nuclease with expanded targeting space." Science 361.6408 (2018): 1259...